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学科主题: Forestry
题名: Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)
作者: Huang, GY ; Wang, YS ; Ying, GG ; Dang, AC
通讯作者: huang_gyh@sina.com
关键词: Kandelia candel ; Mangrove species ; Metal tolerance ; Metallothionein
刊名: TREES-STRUCTURE AND FUNCTION
发表日期: 2012
卷: 26, 期:5, 页:1537-1544
部门归属: [Huang, Guo-Yong; Wang, You-Shao; Dang, Ai-Cui] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Trop Marine Environm Dynam, Guangzhou 510301, Guangdong, Peoples R China; [Huang, Guo-Yong; Ying, Guang-Guo] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China
项目归属: LTO
摘要: Metallothioneins are Cys-rich, low-molecular weight, metal-binding proteins. In this paper, we aimed to evaluate the roles of type 2 metallothionein in Kandelia candel (KcMT2) by analyzing its mRNA expression in leaves in response to heavy metals, as well as the metal-binding properties of its protein. The transcriptional response of KcMT2 gene to different heavy metals (Zn, Cu, Pb and Cd) was measured using real-time quantitative PCR. The results showed that the gene was regulated by the four metals tested. Significant increase in the expression of KcMT2 mRNA was also found in response to Zn, Cu, Pb and Cd. To analyze further the possible roles of KcMT2 against heavy metals, it was expressed in Escherichia coli BL21 as a carboxy-terminal extension of glutathione-S-transferase (GST). The E. coli cells expressing GST-KcMT2 fusion protein showed higher growth compared to the control cells under Zn, Cu, Pb or Cd exposure, and the GST-KcMT2 fusion protein had a stronger binding affinity for Zn, Cu, Pb and Cd than GST alone. Results presented here reveal that KcMT2 may play important roles in the heavy metal homeostasis or detoxification.
语种: 英语
WOS记录号: WOS:000308860500011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10101
Appears in Collections:热带海洋环境国家重点实验室(LTO)_期刊论文

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Recommended Citation:
Huang, GY; Wang, YS; Ying, GG; Dang, AC.Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel),TREES-STRUCTURE AND FUNCTION,2012,26(5):1537-1544
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