Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)
Huang, GY; Wang, YS; Ying, GG; Dang, AC; huang_gyh@sina.com
2012
发表期刊TREES-STRUCTURE AND FUNCTION
ISSN0931-1890
卷号26期号:5页码:1537-1544
摘要Metallothioneins are Cys-rich, low-molecular weight, metal-binding proteins. In this paper, we aimed to evaluate the roles of type 2 metallothionein in Kandelia candel (KcMT2) by analyzing its mRNA expression in leaves in response to heavy metals, as well as the metal-binding properties of its protein. The transcriptional response of KcMT2 gene to different heavy metals (Zn, Cu, Pb and Cd) was measured using real-time quantitative PCR. The results showed that the gene was regulated by the four metals tested. Significant increase in the expression of KcMT2 mRNA was also found in response to Zn, Cu, Pb and Cd. To analyze further the possible roles of KcMT2 against heavy metals, it was expressed in Escherichia coli BL21 as a carboxy-terminal extension of glutathione-S-transferase (GST). The E. coli cells expressing GST-KcMT2 fusion protein showed higher growth compared to the control cells under Zn, Cu, Pb or Cd exposure, and the GST-KcMT2 fusion protein had a stronger binding affinity for Zn, Cu, Pb and Cd than GST alone. Results presented here reveal that KcMT2 may play important roles in the heavy metal homeostasis or detoxification.
部门归属[Huang, Guo-Yong; Wang, You-Shao; Dang, Ai-Cui] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Trop Marine Environm Dynam, Guangzhou 510301, Guangdong, Peoples R China; [Huang, Guo-Yong; Ying, Guang-Guo] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China
关键词Kandelia Candel Mangrove Species Metal Tolerance Metallothionein
学科领域Forestry
语种英语
资助项目LTO
WOS记录号WOS:000308860500011
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10101
专题热带海洋环境国家重点实验室(LTO)
通讯作者huang_gyh@sina.com
推荐引用方式
GB/T 7714
Huang, GY,Wang, YS,Ying, GG,et al. Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)[J]. TREES-STRUCTURE AND FUNCTION,2012,26(5):1537-1544.
APA Huang, GY,Wang, YS,Ying, GG,Dang, AC,&huang_gyh@sina.com.(2012).Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel).TREES-STRUCTURE AND FUNCTION,26(5),1537-1544.
MLA Huang, GY,et al."Analysis of type 2 metallothionein gene from mangrove species (Kandelia candel)".TREES-STRUCTURE AND FUNCTION 26.5(2012):1537-1544.
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