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学科主题: Ecology; Environmental Sciences; Toxicology
题名: A novel metallothionein gene from a mangrove plant Kandelia candel
作者: Zhang, FQ ; Wang, YS ; Sun, CC ; Lou, ZP ; Dong, JD
通讯作者: yswang@scsio.ac.cn
关键词: Kandelia candel ; Metallothionein gene ; Heavy metal tolerance ; RACE
刊名: ECOTOXICOLOGY
发表日期: 2012
卷: 21, 期:6, 页:1633-1641
部门归属: [Zhang, Feng-Qin; Wang, You-Shao; Sun, Cui-Ci; Lou, Zhi-Ping] Chinese Acad Sci, State Key Lab Trop Oceanog, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Feng-Qin; Wang, You-Shao; Sun, Cui-Ci; Lou, Zhi-Ping] Chinese Acad Sci, Daya Bay Marine Biol Res Stn, Shenzhen 518121, Peoples R China; [Dong, Jun-De] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LTO
摘要: A new metallothionein (MT) gene was cloned from Kandelia candel, a mangrove plant with constitutional tolerance to heavy metals, by rapid amplification of cDNA ends and named KMT, which is composed of two exons and one intron. The full length of KMT cDNA was 728 bp including 121 bp 5' noncoding domain, 240 bp open reading frame and 384 bp 3' termination. The coding region of KMT represented a putative 79 amino acid protein with a molecular weight of 7.75 kDa. At each of the amino- and carboxy-terminal of the putative protein, cysteine residues were arranged in Cys-Cys, Cys-X-Cys and Cys-X-X-Cys, indicating that the putative protein was a novel type 2 MT. Sequence and homology analysis showed the KMT protein sequence shared more than 60 % homology with other plant type 2 MT-like protein genes. At amino acid level, the KMT was shown homology with the MT of Quercus suber (83 %), of Ricinus communis (81 %) and of Arabidopsis thaliana (64 %). Function studies using protease-deficient Escherichia coli strain BL21 Star (TM)(DE3) confirmed the functional nature of this KMT gene in sequestering both essential (Zn) and non-essential metals (Cd and Hg) and the E. coli BL21 with KMT can live in 1,000 mu mol/L Zn, 120 mu mol/L Hg, and 2,000 mu mol/L Cd. The information could provide more details of the causative molecular and biochemical mechanisms (including heavy metal sequestration) of the KMT in K. candel or a scientific basis for marine heavy-metal environment remediation with K. candel. This study also provides a great significance of protecting mangrove species and mangrove ecosystem.
语种: 英语
WOS记录号: WOS:000306592100008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10141
Appears in Collections:热带海洋环境国家重点实验室(LTO)_期刊论文

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Zhang, FQ; Wang, YS; Sun, CC; Lou, ZP; Dong, JD.A novel metallothionein gene from a mangrove plant Kandelia candel,ECOTOXICOLOGY,2012,21(6):1633-1641
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