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学科主题: Science & Technology - Other Topics
题名: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene
作者: Li, BY ; Yao, Q ; Zhu, HH
通讯作者: zhuhh@gdim.cn
刊名: PLOS ONE
发表日期: 2014
卷: 9, 期:10, 页:-e108877
收录类别: sci
部门归属: [Li, Baiyuan ; Zhu, Honghui] Guangdong Prov Key Lab Microbial Culture Collect, State Key Lab Appl Microbiol Southern China, Guangdong Inst Microbiol, Guangzhou, Guangdong, Peoples R China ; [Li, Baiyuan] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China ; [Li, Baiyuan] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Yao, Qing] South China Agr Univ, Coll Hort, Guangzhou, Guangdong, Peoples R China
项目归属: LMB
资助者: Natural Science Foundation of China [31170009]; Open Foundation of Tarim University [BRZD1004]
摘要: The genotypic diversity of insoluble macromolecules degraded myxobacteria, provided an opportunity to discover new bacterial resources and find new ecological functions. In this study, we developed a semi-nested-PCR-denaturing gradient gel electrophoresis (DGGE) strategy to determine the presence and genotypic diversity of myxobacteria in soil. After two rounds of PCR with myxobacteria-specific primers, an 194 bp fragment of mglA, a key gene involved in gliding motility, suitable for DGGE was obtained. A large number of bands were observed in DGGE patterns, indicating diverse myxobacteria inhabiting in soils. Furthermore, sequencing and BLAST revealed that most of the bands belonged to the myxobacteria-group, and only three of the twenty-eight bands belonged to other group, i.e., Deinococcus maricopensis. The results verified that myxobacterial strains with discrepant sequence compositions of gene mglA could be discriminated by DGGE with myxobacteria-specific primers. Collectively, the developed semi-nested-PCR-DGGE strategy is a useful tool for studying the diversity of myxobacteria.
原文出处: 查看原文
WOS记录号: WOS:000342670800032
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10371
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Li, BY; Yao, Q; Zhu, HH.Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene,PLOS ONE,2014,9(10):-e108877
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