Insights into Deep-Sea Sediment Fungal Communities from the East Indian Ocean Using Targeted Environmental Sequencing Combined with Traditional Cultivation
Zhang, XY; Tang, GL; Xu, XY; Nong, XH; Qi, SH; shuhuaqi@scsio.ac.cn
2014
发表期刊PLOS ONE
ISSN1932-6203
卷号9期号:10页码:-e109118
摘要The fungal diversity in deep-sea environments has recently gained an increasing amount attention. Our knowledge and understanding of the true fungal diversity and the role it plays in deep-sea environments, however, is still limited. We investigated the fungal community structure in five sediments from a depth of,4000 m in the East India Ocean using a combination of targeted environmental sequencing and traditional cultivation. This approach resulted in the recovery of a total of 45 fungal operational taxonomic units (OTUs) and 20 culturable fungal phylotypes. This finding indicates that there is a great amount of fungal diversity in the deep-sea sediments collected in the East Indian Ocean. Three fungal OTUs and one culturable phylotype demonstrated high divergence (89%-97%) from the existing sequences in the GenBank. Moreover, 44.4% fungal OTUs and 30% culturable fungal phylotypes are new reports for deep-sea sediments. These results suggest that the deep-sea sediments from the East India Ocean can serve as habitats for new fungal communities compared with other deep-sea environments. In addition, different fungal community could be detected when using targeted environmental sequencing compared with traditional cultivation in this study, which suggests that a combination of targeted environmental sequencing and traditional cultivation will generate a more diverse fungal community in deep-sea environments than using either targeted environmental sequencing or traditional cultivation alone. This study is the first to report new insights into the fungal communities in deep-sea sediments from the East Indian Ocean, which increases our knowledge and understanding of the fungal diversity in deep-sea environments.
部门归属[Zhang, Xiao-yong ; Tang, Gui-ling ; Xu, Xin-ya ; Nong, Xu-hua ; Qi, Shu-Hua] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Key Lab Trop Marine Bioresources & Ecol,RNAM Ctr, Guangzhou, Guangdong, Peoples R China
学科领域Science & Technology - Other Topics
资助者National Basic Research Program of China [2010CB833803]; National High Technology Research and Development Program of China (863 Program) [2012AA092104]; Natural Science Foundation of China [41206139, 41376160]; regional innovation demonstration project of Guangdong Province marine economic development [GD2012-D01-002]; National Marine Public Welfare Research Project of China [201305017]; National Key Technologies RD Program [2011BAE06B04-03] ; National Basic Research Program of China [2010CB833803]; National High Technology Research and Development Program of China (863 Program) [2012AA092104]; Natural Science Foundation of China [41206139, 41376160]; regional innovation demonstration project of Guangdong Province marine economic development [GD2012-D01-002]; National Marine Public Welfare Research Project of China [201305017]; National Key Technologies RD Program [2011BAE06B04-03] ; National Basic Research Program of China [2010CB833803]; National High Technology Research and Development Program of China (863 Program) [2012AA092104]; Natural Science Foundation of China [41206139, 41376160]; regional innovation demonstration project of Guangdong Province marine economic development [GD2012-D01-002]; National Marine Public Welfare Research Project of China [201305017]; National Key Technologies RD Program [2011BAE06B04-03] ; National Basic Research Program of China [2010CB833803]; National High Technology Research and Development Program of China (863 Program) [2012AA092104]; Natural Science Foundation of China [41206139, 41376160]; regional innovation demonstration project of Guangdong Province marine economic development [GD2012-D01-002]; National Marine Public Welfare Research Project of China [201305017]; National Key Technologies RD Program [2011BAE06B04-03]
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收录类别sci
资助项目LMB
资助者National Basic Research Program of China [2010CB833803]; National High Technology Research and Development Program of China (863 Program) [2012AA092104]; Natural Science Foundation of China [41206139, 41376160]; regional innovation demonstration project of Guangdong Province marine economic development [GD2012-D01-002]; National Marine Public Welfare Research Project of China [201305017]; National Key Technologies RD Program [2011BAE06B04-03] ; National Basic Research Program of China [2010CB833803]; National High Technology Research and Development Program of China (863 Program) [2012AA092104]; Natural Science Foundation of China [41206139, 41376160]; regional innovation demonstration project of Guangdong Province marine economic development [GD2012-D01-002]; National Marine Public Welfare Research Project of China [201305017]; National Key Technologies RD Program [2011BAE06B04-03] ; National Basic Research Program of China [2010CB833803]; National High Technology Research and Development Program of China (863 Program) [2012AA092104]; Natural Science Foundation of China [41206139, 41376160]; regional innovation demonstration project of Guangdong Province marine economic development [GD2012-D01-002]; National Marine Public Welfare Research Project of China [201305017]; National Key Technologies RD Program [2011BAE06B04-03] ; National Basic Research Program of China [2010CB833803]; National High Technology Research and Development Program of China (863 Program) [2012AA092104]; Natural Science Foundation of China [41206139, 41376160]; regional innovation demonstration project of Guangdong Province marine economic development [GD2012-D01-002]; National Marine Public Welfare Research Project of China [201305017]; National Key Technologies RD Program [2011BAE06B04-03]
WOS记录号WOS:000343729600098
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10372
专题中科院海洋生物资源可持续利用重点实验室
通讯作者shuhuaqi@scsio.ac.cn
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Zhang, XY,Tang, GL,Xu, XY,et al. Insights into Deep-Sea Sediment Fungal Communities from the East Indian Ocean Using Targeted Environmental Sequencing Combined with Traditional Cultivation[J]. PLOS ONE,2014,9(10):-e109118.
APA Zhang, XY,Tang, GL,Xu, XY,Nong, XH,Qi, SH,&shuhuaqi@scsio.ac.cn.(2014).Insights into Deep-Sea Sediment Fungal Communities from the East Indian Ocean Using Targeted Environmental Sequencing Combined with Traditional Cultivation.PLOS ONE,9(10),-e109118.
MLA Zhang, XY,et al."Insights into Deep-Sea Sediment Fungal Communities from the East Indian Ocean Using Targeted Environmental Sequencing Combined with Traditional Cultivation".PLOS ONE 9.10(2014):-e109118.
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