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学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Genomic characterization and expression analysis of five novel IL-17 genes in the Pacific oyster, Crassostrea gigas
作者: Li, J ; Zhang, Y ; Zhang, YH ; Xiang, ZM ; Tong, Y ; Qu, FF ; Yu, ZN
通讯作者: carlzyu@scsio.ac.cn
关键词: Interleukin-17 ; Genomic organization ; PAMPs challenge ; Pacific oyster
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2014
卷: 40, 期:2, 页:455-465
收录类别: sci
部门归属: [Li, Jun ; Zhang, Yang ; Zhang, Yuehuan ; Xiang, Zhiming ; Tong, Ying ; Qu, Fufa ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150, 41306145]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]; NSFC-Guangdong of China [U1201215]
摘要: Interleukin-17 (IL-17) is a proinflammatory cytokine that plays an important role in clearing extracellular bacteria and contributes to the pathology of many autoimmune and allergic conditions. In the present study, five novel IL-17 homologs were identified by searching and analyzing the Pacific oyster genome. All six CgIL-17 members (including a previously reported homolog) contained four conserved cysteines that were used in the formation of disulfide bonds. Phylogenetic analysis showed that all invertebrate IL-17s were clustered into one group, implying that invertebrate IL-17s evolved from one common ancestral gene and subsequently diversified. All CgIL-17s shared the same genomic structure, containing two exons and one intron, except for the CgIL-17-3 and CgIL-17-5 genes, which each had only one exon. The expression pattern of the CgIL-17 genes was analyzed by gRT-PCR in a variety of tissues and at different developmental stages, and these genes were highly expressed in the gill and digestive gland tissues. Moreover, the expression of the CgIL-17 family genes was significantly up-regulated in hemocytes challenged with Pathogen-Associated Molecular Patterns (PAMPs). CgIL-17-3 had a strong response to lipopolysaccharide (LPS) and heat-killed Vibrio alginolyticus (HKVA) challenge, while CgIL-17-5 and CgIL-17-6 can be activated by peptidoglycan (PGN), but not by heat-killed Listeria monogtogenes (HKLM). The distinct, up-regulated transcript levels of the CgIL-17s in response to PAMPs challenge further indicate that CgIL-17s are likely to be significant components of immune responses by playing diversified roles in host defense in the Pacific oyster. These findings suggest that CgIL-17s are involved in innate immune responses and further supports their conserved function in mollusks immunity. (C) 2014 Elsevier Ltd. All rights reserved.
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WOS记录号: WOS:000343381100014
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10374
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Li, J; Zhang, Y; Zhang, YH; Xiang, ZM; Tong, Y; Qu, FF; Yu, ZN.Genomic characterization and expression analysis of five novel IL-17 genes in the Pacific oyster, Crassostrea gigas,FISH & SHELLFISH IMMUNOLOGY,2014,40(2):455-465
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