Genomic characterization and expression analysis of five novel IL-17 genes in the Pacific oyster, Crassostrea gigas
Li, J; Zhang, Y; Zhang, YH; Xiang, ZM; Tong, Y; Qu, FF; Yu, ZN; carlzyu@scsio.ac.cn
2014
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号40期号:2页码:455-465
摘要Interleukin-17 (IL-17) is a proinflammatory cytokine that plays an important role in clearing extracellular bacteria and contributes to the pathology of many autoimmune and allergic conditions. In the present study, five novel IL-17 homologs were identified by searching and analyzing the Pacific oyster genome. All six CgIL-17 members (including a previously reported homolog) contained four conserved cysteines that were used in the formation of disulfide bonds. Phylogenetic analysis showed that all invertebrate IL-17s were clustered into one group, implying that invertebrate IL-17s evolved from one common ancestral gene and subsequently diversified. All CgIL-17s shared the same genomic structure, containing two exons and one intron, except for the CgIL-17-3 and CgIL-17-5 genes, which each had only one exon. The expression pattern of the CgIL-17 genes was analyzed by gRT-PCR in a variety of tissues and at different developmental stages, and these genes were highly expressed in the gill and digestive gland tissues. Moreover, the expression of the CgIL-17 family genes was significantly up-regulated in hemocytes challenged with Pathogen-Associated Molecular Patterns (PAMPs). CgIL-17-3 had a strong response to lipopolysaccharide (LPS) and heat-killed Vibrio alginolyticus (HKVA) challenge, while CgIL-17-5 and CgIL-17-6 can be activated by peptidoglycan (PGN), but not by heat-killed Listeria monogtogenes (HKLM). The distinct, up-regulated transcript levels of the CgIL-17s in response to PAMPs challenge further indicate that CgIL-17s are likely to be significant components of immune responses by playing diversified roles in host defense in the Pacific oyster. These findings suggest that CgIL-17s are involved in innate immune responses and further supports their conserved function in mollusks immunity. (C) 2014 Elsevier Ltd. All rights reserved.
部门归属[Li, Jun ; Zhang, Yang ; Zhang, Yuehuan ; Xiang, Zhiming ; Tong, Ying ; Qu, Fufa ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
关键词Interleukin-17 Genomic Organization Pamps Challenge Pacific Oyster
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150, 41306145]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]; NSFC-Guangdong of China [U1201215] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150, 41306145]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]; NSFC-Guangdong of China [U1201215] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150, 41306145]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]; NSFC-Guangdong of China [U1201215] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150, 41306145]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]; NSFC-Guangdong of China [U1201215]
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收录类别sci
资助项目LMB
资助者National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150, 41306145]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]; NSFC-Guangdong of China [U1201215] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150, 41306145]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]; NSFC-Guangdong of China [U1201215] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150, 41306145]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]; NSFC-Guangdong of China [U1201215] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150, 41306145]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]; NSFC-Guangdong of China [U1201215]
WOS记录号WOS:000343381100014
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10374
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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Li, J,Zhang, Y,Zhang, YH,et al. Genomic characterization and expression analysis of five novel IL-17 genes in the Pacific oyster, Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(2):455-465.
APA Li, J.,Zhang, Y.,Zhang, YH.,Xiang, ZM.,Tong, Y.,...&carlzyu@scsio.ac.cn.(2014).Genomic characterization and expression analysis of five novel IL-17 genes in the Pacific oyster, Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,40(2),455-465.
MLA Li, J,et al."Genomic characterization and expression analysis of five novel IL-17 genes in the Pacific oyster, Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 40.2(2014):455-465.
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