The first invertebrate RIG-I-like receptor (RLR) homolog gene in the pacific oyster Crassostrea gigas
Zhang, Y; Yu, F; Li, J; Tong, Y; Zhang, YH; Yu, ZN; carlzyu@scsio.ac.cn
2014
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号40期号:2页码:466-471
摘要Retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) is a pivotal receptor that detects numerous RNA and DNA viruses and mediates the innate induction of interferons and pro-inflammatory cytokines upon viral infection. In the present study, we cloned and characterized the first RIG-I gene in a marine mollusk, Crassostrea gigas, and designated it as CgRIG-I. The full-length CgRIG-I cDNA is 3436 bp, including 5'- and 3'-untranslated regions (UTRs) of 93 bp and 286 bp, respectively, and an open reading frame (ORF) of 3057 bp. The gene encodes a 1018 amino acid polypeptide with an estimated molecular mass of 116.5 kDa. SMART analysis showed that the CgRIG-I protein had the typical conserved domains, including the caspase activation and recruitment domains (CARDs), the RNA helicase domain and the C-terminal regulatory domain (RD). Phylogenetic analysis revealed that CgRIG-I was grouped into the clade of its vertebrate homologs. Moreover, CgRIG-I expression could be specifically increased after stimulation by poly(I:C) rather than by other PAMPs such as lipopolysaccharide (LPS), peptidoglycan (PGN), heat-killed Listeria monocytogenes (HKLM) and heat-killed Vibrio alginolyticus (HKVA). Meanwhile, six IRF, three STAT and one NF-kappa B predicted sites were identified in the CgRIG-I promoter, which was consistent with its high responsiveness to poly(I:C). In summary, this report provides the first CgRIG-I sequence of a mollusk, but its function in the antiviral immune response requires further investigation. (C) 2014 Elsevier Ltd. All rights reserved.
部门归属[Zhang, Yang ; Yu, Feng ; Li, Jun ; Tong, Ying ; Zhang, Yuehuan ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China
关键词Crassostrea Gigas Retinoic Acid-inducible Gene i Antiviral Immune Response Pamp Challenge
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者National Basic Research Program of China [2010CB126404]; National Science Foundation of China [41176150]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Science Foundation of China [41176150]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Science Foundation of China [41176150]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Science Foundation of China [41176150]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]
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收录类别sci
资助项目LMB
资助者National Basic Research Program of China [2010CB126404]; National Science Foundation of China [41176150]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Science Foundation of China [41176150]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Science Foundation of China [41176150]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Science Foundation of China [41176150]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]
WOS记录号WOS:000343381100015
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10375
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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Zhang, Y,Yu, F,Li, J,et al. The first invertebrate RIG-I-like receptor (RLR) homolog gene in the pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(2):466-471.
APA Zhang, Y.,Yu, F.,Li, J.,Tong, Y.,Zhang, YH.,...&carlzyu@scsio.ac.cn.(2014).The first invertebrate RIG-I-like receptor (RLR) homolog gene in the pacific oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,40(2),466-471.
MLA Zhang, Y,et al."The first invertebrate RIG-I-like receptor (RLR) homolog gene in the pacific oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 40.2(2014):466-471.
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