Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in beta-carboline skeleton biosynthesis
Wang, H; Zhang, HD; Mi, YL; Ju, JH; Chen, Q; Zhang, HJ; hjzhang@hust.edu.cn
2014
发表期刊ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
ISSN1744-3091
卷号70页码:1402-1405
摘要beta-Carboline alkaloids (beta Cs), with tricyclic pyrido[3,4-b] indole rings, have important pharmacological and therapeutic value. In the biosynthesis of beta Cs, the Pictet-Spengler (PS) cyclization reaction is responsible for the formation of ring structures. McbB is one of a few enzymes that are known to catalyse PS cyclization. It can also catalyse decarboxylation and oxidation. Here, the expression, crystallization and preliminary data analysis of McbB are reported. The crystals diffracted to 2.10 angstrom resolution and belonged to the monoclinic space group P2(1), with unit-cell parameters a = 66.06, b = 85.48, c = 106.19 angstrom, alpha = 90.00, beta = 106.77, gamma = 90.00 degrees. These results provide a basis for solving the crystal structure and elucidating the catalytic mechanism for McbB.
部门归属[Wang, Hua ; Zhang, Huaidong ; Mi, Yanling ; Zhang, Houjin] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Dept Biotechnol, Wuhan 430074, Hubei, Peoples R China ; [Ju, Jianhua ; Chen, Qi] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol Microbiol,Guangdong, South China Sea Inst Oceanol,RNAM Ctr Marine Micr, Guangzhou 510301, Guangdong, Peoples R China
学科领域Biochemistry & Molecular Biology ; Biophysics ; Crystallography
资助者National Key Basic Research Program of China [2013CB933900]; National Natural Science Foundation of China [31000326] ; National Key Basic Research Program of China [2013CB933900]; National Natural Science Foundation of China [31000326] ; National Key Basic Research Program of China [2013CB933900]; National Natural Science Foundation of China [31000326] ; National Key Basic Research Program of China [2013CB933900]; National Natural Science Foundation of China [31000326]
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收录类别sci
资助项目LMB
资助者National Key Basic Research Program of China [2013CB933900]; National Natural Science Foundation of China [31000326] ; National Key Basic Research Program of China [2013CB933900]; National Natural Science Foundation of China [31000326] ; National Key Basic Research Program of China [2013CB933900]; National Natural Science Foundation of China [31000326] ; National Key Basic Research Program of China [2013CB933900]; National Natural Science Foundation of China [31000326]
WOS记录号WOS:000343060200020
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10377
专题中科院海洋生物资源可持续利用重点实验室
通讯作者hjzhang@hust.edu.cn
推荐引用方式
GB/T 7714
Wang, H,Zhang, HD,Mi, YL,et al. Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in beta-carboline skeleton biosynthesis[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2014,70:1402-1405.
APA Wang, H.,Zhang, HD.,Mi, YL.,Ju, JH.,Chen, Q.,...&hjzhang@hust.edu.cn.(2014).Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in beta-carboline skeleton biosynthesis.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,70,1402-1405.
MLA Wang, H,et al."Expression, crystallization and preliminary X-ray analysis of McbB, a multifunctional enzyme involved in beta-carboline skeleton biosynthesis".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70(2014):1402-1405.
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