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学科主题: Microbiology
题名: Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes
作者: Li, J ; Dong, JD ; Yang, J ; Luo, XM ; Zhang, S
通讯作者: zhsimd@scsio.ac.cn
关键词: Scleractinian coral ; Actinomycetes ; Antimicrobial activity ; PKS ; NRPS
刊名: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
发表日期: 2014
卷: 106, 期:4, 页:623-635
收录类别: sci
部门归属: [Li, Jie ; Dong, Jun-De ; Yang, Jian ; Luo, Xiong-Ming ; Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, RNAM Ctr Marine Microbiol, CAS Key Lab Trop Marine Bioresources & Ecol,Guang, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
资助者: Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301]
摘要: The diversity and properties of actinobacteria, predominant residents in coral holobionts, have been rarely documented. In this study, we aimed to explore the species diversity, antimicrobial activities and biosynthetic potential of culturable actinomycetes within the tissues of the scleractinian corals Porites lutea, Galaxea fascicularis and Acropora millepora from the South China Sea. A total of 70 strains representing 13 families and 15 genera of actinobacteria were isolated. The antimicrobial activity and biosynthetic potential of fifteen representative filamentous actinomycetes were estimated. Crude fermentation extracts of 6 strains exhibited comparable or greater activities against Vibrio alginolyticus than ciprofloxacin. Seven of the 15 actinomycetes strains possess type I polyketide synthases (PKS-I) and/or nonribosomal peptide synthetases (NRPS) genes. Nine tested strains possess type II polyketide synthases (PKS-II). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these PKS and NRPS gene screening positive strains belong to genera Nocardiopsis, Pseudonocardia, Streptomyces, Micromonospora, Amycolatopsis and Prauserella. One PKS-I and four NRPS fragments showed < 70 % similarity to their closest relatives, which suggested the novelty of these genes. This study helps uncover the genetic capacity of stony coral-associated actinomycetes to produce bioactive molecules.
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WOS记录号: WOS:000341859100004
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10382
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Li, J; Dong, JD; Yang, J; Luo, XM; Zhang, S.Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes,ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2014,106(4):623-635
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