Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes
Li, J; Dong, JD; Yang, J; Luo, XM; Zhang, S; zhsimd@scsio.ac.cn
2014
发表期刊ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN0003-6072
卷号106期号:4页码:623-635
摘要The diversity and properties of actinobacteria, predominant residents in coral holobionts, have been rarely documented. In this study, we aimed to explore the species diversity, antimicrobial activities and biosynthetic potential of culturable actinomycetes within the tissues of the scleractinian corals Porites lutea, Galaxea fascicularis and Acropora millepora from the South China Sea. A total of 70 strains representing 13 families and 15 genera of actinobacteria were isolated. The antimicrobial activity and biosynthetic potential of fifteen representative filamentous actinomycetes were estimated. Crude fermentation extracts of 6 strains exhibited comparable or greater activities against Vibrio alginolyticus than ciprofloxacin. Seven of the 15 actinomycetes strains possess type I polyketide synthases (PKS-I) and/or nonribosomal peptide synthetases (NRPS) genes. Nine tested strains possess type II polyketide synthases (PKS-II). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these PKS and NRPS gene screening positive strains belong to genera Nocardiopsis, Pseudonocardia, Streptomyces, Micromonospora, Amycolatopsis and Prauserella. One PKS-I and four NRPS fragments showed < 70 % similarity to their closest relatives, which suggested the novelty of these genes. This study helps uncover the genetic capacity of stony coral-associated actinomycetes to produce bioactive molecules.
部门归属[Li, Jie ; Dong, Jun-De ; Yang, Jian ; Luo, Xiong-Ming ; Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, RNAM Ctr Marine Microbiol, CAS Key Lab Trop Marine Bioresources & Ecol,Guang, Guangzhou 510301, Guangdong, Peoples R China
关键词Scleractinian Coral Actinomycetes Antimicrobial Activity Pks Nrps
学科领域Microbiology
资助者Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301]
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收录类别sci
资助项目LMB
资助者Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301]
WOS记录号WOS:000341859100004
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10382
专题中科院海洋生物资源可持续利用重点实验室
通讯作者zhsimd@scsio.ac.cn
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GB/T 7714
Li, J,Dong, JD,Yang, J,et al. Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2014,106(4):623-635.
APA Li, J,Dong, JD,Yang, J,Luo, XM,Zhang, S,&zhsimd@scsio.ac.cn.(2014).Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,106(4),623-635.
MLA Li, J,et al."Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 106.4(2014):623-635.
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