SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes
Li, J; Dong, JD; Yang, J; Luo, XM; Zhang, S; zhsimd@scsio.ac.cn
2014
Source PublicationANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN0003-6072
Volume106Issue:4Pages:623-635
AbstractThe diversity and properties of actinobacteria, predominant residents in coral holobionts, have been rarely documented. In this study, we aimed to explore the species diversity, antimicrobial activities and biosynthetic potential of culturable actinomycetes within the tissues of the scleractinian corals Porites lutea, Galaxea fascicularis and Acropora millepora from the South China Sea. A total of 70 strains representing 13 families and 15 genera of actinobacteria were isolated. The antimicrobial activity and biosynthetic potential of fifteen representative filamentous actinomycetes were estimated. Crude fermentation extracts of 6 strains exhibited comparable or greater activities against Vibrio alginolyticus than ciprofloxacin. Seven of the 15 actinomycetes strains possess type I polyketide synthases (PKS-I) and/or nonribosomal peptide synthetases (NRPS) genes. Nine tested strains possess type II polyketide synthases (PKS-II). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these PKS and NRPS gene screening positive strains belong to genera Nocardiopsis, Pseudonocardia, Streptomyces, Micromonospora, Amycolatopsis and Prauserella. One PKS-I and four NRPS fragments showed < 70 % similarity to their closest relatives, which suggested the novelty of these genes. This study helps uncover the genetic capacity of stony coral-associated actinomycetes to produce bioactive molecules.
Department[Li, Jie ; Dong, Jun-De ; Yang, Jian ; Luo, Xiong-Ming ; Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, RNAM Ctr Marine Microbiol, CAS Key Lab Trop Marine Bioresources & Ecol,Guang, Guangzhou 510301, Guangdong, Peoples R China
KeywordScleractinian Coral Actinomycetes Antimicrobial Activity Pks Nrps
Subject AreaMicrobiology
Funding OrganizationPearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301]
URL查看原文
Indexed Bysci
Funding ProjectLMB
Funding OrganizationPearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301] ; Pearl River Nova Program of Guangzhou [2014J2200075]; National Natural Science Foundation of China [41106139, 41230962]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201301]
WOS IDWOS:000341859100004
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10382
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorzhsimd@scsio.ac.cn
Recommended Citation
GB/T 7714
Li, J,Dong, JD,Yang, J,et al. Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2014,106(4):623-635.
APA Li, J,Dong, JD,Yang, J,Luo, XM,Zhang, S,&zhsimd@scsio.ac.cn.(2014).Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,106(4),623-635.
MLA Li, J,et al."Detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 106.4(2014):623-635.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.