Antifungal effect and mechanism of garlic oil on Penicillium funiculosum
Li, WR; Shi, QS; Liang, Q; Huang, XM; Chen, YB; jigan@gdim.cn
2014
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
卷号98期号:19页码:8337-8346
摘要Garlic oil is a kind of fungicide, but little is known about its antifungal effects and mechanism. In this study, the chemical constituents, antifungal activity, and effects of garlic oil were studied with Penicillium funiculosum as a model strain. Results showed that the minimum fungicidal concentrations (MFCs, v/v) were 0.125 and 0.0313 % in agar medium and broth medium, respectively, suggesting that the garlic oil had a strong antifungal activity. The main ingredients of garlic oil were identified as sulfides, mainly including disulfides (36 %), trisulfides (32 %) and monosulfides (29 %) by gas chromatograph-mass spectrometer (GC/MS), which were estimated as the dominant antifungal factors. The observation results by transmission electron microscope (TEM) and scanning electron microscope (SEM) indicated that garlic oil could firstly penetrate into hyphae cells and even their organelles, and then destroy the cellular structure, finally leading to the leakage of both cytoplasm and macromolecules. Further proteomic analysis displayed garlic oil was able to induce a stimulated or weakened expression of some key proteins for physiological metabolism. Therefore, our study proved that garlic oil can work multiple sites of the hyphae of P. funiculosum to cause their death. The high antifungal effects of garlic oil makes it a broad application prospect in antifungal industries.
部门归属[Li, Wen-Ru ; Shi, Qing-Shan ; Liang, Qing ; Huang, Xiao-Mo ; Chen, Yi-Ben] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, State Key Lab Appl Microbiol Southern China, Guangzhou 510070, Guangdong, Peoples R China ; [Liang, Qing] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
关键词Garlic Oil Antifungal Effects Antifungal Mechanism Penicillium Funiculosum
学科领域Biotechnology & Applied Microbiology
资助者Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115]
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收录类别sci
资助项目LMB
资助者Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115]
WOS记录号WOS:000342072500024
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10383
专题中科院海洋生物资源可持续利用重点实验室
通讯作者jigan@gdim.cn
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GB/T 7714
Li, WR,Shi, QS,Liang, Q,et al. Antifungal effect and mechanism of garlic oil on Penicillium funiculosum[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(19):8337-8346.
APA Li, WR,Shi, QS,Liang, Q,Huang, XM,Chen, YB,&jigan@gdim.cn.(2014).Antifungal effect and mechanism of garlic oil on Penicillium funiculosum.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(19),8337-8346.
MLA Li, WR,et al."Antifungal effect and mechanism of garlic oil on Penicillium funiculosum".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.19(2014):8337-8346.
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