SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Antifungal effect and mechanism of garlic oil on Penicillium funiculosum
Li, WR; Shi, QS; Liang, Q; Huang, XM; Chen, YB; jigan@gdim.cn
2014
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
Volume98Issue:19Pages:8337-8346
AbstractGarlic oil is a kind of fungicide, but little is known about its antifungal effects and mechanism. In this study, the chemical constituents, antifungal activity, and effects of garlic oil were studied with Penicillium funiculosum as a model strain. Results showed that the minimum fungicidal concentrations (MFCs, v/v) were 0.125 and 0.0313 % in agar medium and broth medium, respectively, suggesting that the garlic oil had a strong antifungal activity. The main ingredients of garlic oil were identified as sulfides, mainly including disulfides (36 %), trisulfides (32 %) and monosulfides (29 %) by gas chromatograph-mass spectrometer (GC/MS), which were estimated as the dominant antifungal factors. The observation results by transmission electron microscope (TEM) and scanning electron microscope (SEM) indicated that garlic oil could firstly penetrate into hyphae cells and even their organelles, and then destroy the cellular structure, finally leading to the leakage of both cytoplasm and macromolecules. Further proteomic analysis displayed garlic oil was able to induce a stimulated or weakened expression of some key proteins for physiological metabolism. Therefore, our study proved that garlic oil can work multiple sites of the hyphae of P. funiculosum to cause their death. The high antifungal effects of garlic oil makes it a broad application prospect in antifungal industries.
Department[Li, Wen-Ru ; Shi, Qing-Shan ; Liang, Qing ; Huang, Xiao-Mo ; Chen, Yi-Ben] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, State Key Lab Appl Microbiol Southern China, Guangzhou 510070, Guangdong, Peoples R China ; [Liang, Qing] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
KeywordGarlic Oil Antifungal Effects Antifungal Mechanism Penicillium Funiculosum
Subject AreaBiotechnology & Applied Microbiology
Funding OrganizationGuangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115]
URL查看原文
Indexed Bysci
Funding ProjectLMB
Funding OrganizationGuangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115] ; Guangdong Provincial Science and Technology Project [2009B011000011, 2011B010400039]; Foshan City; Chinese Academy of Sciences [2012HY100115]
WOS IDWOS:000342072500024
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10383
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorjigan@gdim.cn
Recommended Citation
GB/T 7714
Li, WR,Shi, QS,Liang, Q,et al. Antifungal effect and mechanism of garlic oil on Penicillium funiculosum[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(19):8337-8346.
APA Li, WR,Shi, QS,Liang, Q,Huang, XM,Chen, YB,&jigan@gdim.cn.(2014).Antifungal effect and mechanism of garlic oil on Penicillium funiculosum.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(19),8337-8346.
MLA Li, WR,et al."Antifungal effect and mechanism of garlic oil on Penicillium funiculosum".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.19(2014):8337-8346.
Files in This Item:
File Name/Size DocType Version Access License
Antifungal effect an(1447KB) 开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.