中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Immunology ; Zoology
题名: Molecular cloning and characterization of a new G-type lysozyme gene (Ec-lysG) in orange-spotted grouper, Epinephelus coioides
作者: Wei, SN ; Huang, YH ; Huang, XH ; Cai, J ; Wei, JG ; Li, PF ; Ouyang, ZL ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
关键词: Epinephelus coioides ; G-type lysozyme ; Antimicrobial activity ; Innate immunity
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2014
卷: 46, 期:2, 页:401-412
收录类别: sci
部门归属: [Wei, Shina ; Huang, Youhua ; Huang, Xiaohong ; Cai, Jia ; Wei, Jingguang ; Li, Pengfei ; Ouyang, Zhengliang ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Cai, Jia] Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China ; [Li, Pengfei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China (973) [2012CB114402]; National High Technology Development Program of China (863) [2014AA093507, 2012AA092201]; Chinese Academy of Sciences [XDA11020203]
摘要: Lysozyme acts as an innate immunity molecule against pathogen infection. In this study, a new G-type lysozyme gene with a typical G-type lysozyme domain (designated as Ec-lysG) was cloned and characterized from the orange-spotted grouper, Epinephelus coioides. The full-length Ec-lysG cDNA contains 1419 bp and encodes a 256-residue protein containing a 25-residue signal peptide at the N-terminus. BLAST analysis reveals Ec-lysG shares 64% identity with Siniperca chuatsi, but 63% to another reported G-type lysozyme from orange-spotted grouper (OSG-lysG). The genomic DNA of Ec-lysG contains four exons and three introns, with a total length of 2062 bp. An amino acid sequence alignment showed that Ec-lysG shares the fundamental structural features of G-type lysozyme, including the catalytic residues, substrate binding sites, and soluble lytic transglycosylase domain. Quantitative PCR showed that Ec-lysG transcript is most abundant in the head kidney, and less abundant in the heart. The expression of Ec-lysG was differentially upregulated in the head kidney after stimulation with lipopolysaccharide, Vibrio alginolyticus, and Singapore grouper iridovirus (SGIV). A subcellular localization analysis showed that Ec-lysG is distributed predominantly in the cytoplasm. Recombinant Ec-lysG (rEc-lysG) has optimal activity at pH 7.5 and 35 degrees C. rEc-lysG showed lytic activities against Gram-positive bacterium Streptococcus iniae, Staphylococcus aureus, and Micrococcus lysodeikticus, and the Gram-negative bacterium V. alginolyticus. Scanning electron microscopy (SEM) showed that rEc-lysG acts on M. lysodeikticus cell walls. The overexpression of Ec-lysG in grouper cells did not significantly delay the occurrence of the cytopathic effect (CPE) induced by SGIV, and did not inhibit viral gene transcription. In conclusion, Ec-lysG might be a potent antibacterial protein, with a role in innate immunity. (C) 2014 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000340216600031
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10384
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
1-s2.0-S0145305X14001426-main.pdf(3635KB)----开放获取View Download

Recommended Citation:
Wei, SN; Huang, YH; Huang, XH; Cai, J; Wei, JG; Li, PF; Ouyang, ZL; Qin, QW.Molecular cloning and characterization of a new G-type lysozyme gene (Ec-lysG) in orange-spotted grouper, Epinephelus coioides,DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,46(2):401-412
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wei, SN]'s Articles
[Huang, YH]'s Articles
[Huang, XH]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wei, SN]‘s Articles
[Huang, YH]‘s Articles
[Huang, XH]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 1-s2.0-S0145305X14001426-main.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace