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Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity
Zhang, Y; Li, J; Yu, F; Tong, Y; Zhang, YH; Xiang, ZM; Yu, ZN; carlzyu@scsio.ac.cn
2014
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume40Issue:1Pages:239-244
AbstractSIMPL (Signaling Molecule that associates with the mouse Pelle-Like Kinase) has been recently identified as a co-regulator of NF-kappa B dependent transcription. Here, we report the discovery and functional analysis of the SIMPL in a mollusk, Crassostrea gigas, which terms as CgSIMPL. CgSIMPL is comprised of 252 amino acids and shares significant homology with vertebrate homologs. Over-expression of CgSIMPL does not activate the NF-kappa B reporter in the HEK293 cell line, but can enhance Rel-dependent NF-kappa B transactivation. The dominant-negative effect of CgSIMPL was observed after the deletion of NLS, strongly suggesting that NLS is required for the enhancement of Rel-dependent NF-kappa B transactivation. Furthermore, CgSIMPL mRNA is constitutively expressed in various tissues and is inducible at late stages of infection, supporting its regulatory function in innate immunity. Altogether, our studies reveal that SIMPL is reserved and co-evolved with NF-kappa B in chordate and mollusk, supporting its ancient origin and involvement in regulation of NF-kappa B signaling pathway. (C) 2014 Elsevier Ltd. All rights reserved.
Department[Zhang, Yang ; Li, Jun ; Yu, Feng ; Tong, Ying ; Zhang, Yuehuan ; Xiang, Zhiming ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
KeywordCrassostrea Gigas Simpl Ancient Origin Rel/nf-kappa b Transcription Activity
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationNational Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]
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Indexed Bysci
Funding ProjectLMB
Funding OrganizationNational Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]
WOS IDWOS:000341468500028
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Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10389
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorcarlzyu@scsio.ac.cn
Recommended Citation
GB/T 7714
Zhang, Y,Li, J,Yu, F,et al. Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(1):239-244.
APA Zhang, Y.,Li, J.,Yu, F.,Tong, Y.,Zhang, YH.,...&carlzyu@scsio.ac.cn.(2014).Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity.FISH & SHELLFISH IMMUNOLOGY,40(1),239-244.
MLA Zhang, Y,et al."Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity".FISH & SHELLFISH IMMUNOLOGY 40.1(2014):239-244.
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