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学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity
作者: Zhang, Y ; Li, J ; Yu, F ; Tong, Y ; Zhang, YH ; Xiang, ZM ; Yu, ZN
通讯作者: carlzyu@scsio.ac.cn
关键词: Crassostrea gigas ; SIMPL ; Ancient origin ; Rel/NF-kappa B transcription activity
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2014
卷: 40, 期:1, 页:239-244
收录类别: sci
部门归属: [Zhang, Yang ; Li, Jun ; Yu, Feng ; Tong, Ying ; Zhang, Yuehuan ; Xiang, Zhiming ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]
摘要: SIMPL (Signaling Molecule that associates with the mouse Pelle-Like Kinase) has been recently identified as a co-regulator of NF-kappa B dependent transcription. Here, we report the discovery and functional analysis of the SIMPL in a mollusk, Crassostrea gigas, which terms as CgSIMPL. CgSIMPL is comprised of 252 amino acids and shares significant homology with vertebrate homologs. Over-expression of CgSIMPL does not activate the NF-kappa B reporter in the HEK293 cell line, but can enhance Rel-dependent NF-kappa B transactivation. The dominant-negative effect of CgSIMPL was observed after the deletion of NLS, strongly suggesting that NLS is required for the enhancement of Rel-dependent NF-kappa B transactivation. Furthermore, CgSIMPL mRNA is constitutively expressed in various tissues and is inducible at late stages of infection, supporting its regulatory function in innate immunity. Altogether, our studies reveal that SIMPL is reserved and co-evolved with NF-kappa B in chordate and mollusk, supporting its ancient origin and involvement in regulation of NF-kappa B signaling pathway. (C) 2014 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000341468500028
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10389
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Zhang, Y; Li, J; Yu, F; Tong, Y; Zhang, YH; Xiang, ZM; Yu, ZN.Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity,FISH & SHELLFISH IMMUNOLOGY,2014,40(1):239-244
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