Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity
Zhang, Y; Li, J; Yu, F; Tong, Y; Zhang, YH; Xiang, ZM; Yu, ZN; carlzyu@scsio.ac.cn
2014
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号40期号:1页码:239-244
摘要SIMPL (Signaling Molecule that associates with the mouse Pelle-Like Kinase) has been recently identified as a co-regulator of NF-kappa B dependent transcription. Here, we report the discovery and functional analysis of the SIMPL in a mollusk, Crassostrea gigas, which terms as CgSIMPL. CgSIMPL is comprised of 252 amino acids and shares significant homology with vertebrate homologs. Over-expression of CgSIMPL does not activate the NF-kappa B reporter in the HEK293 cell line, but can enhance Rel-dependent NF-kappa B transactivation. The dominant-negative effect of CgSIMPL was observed after the deletion of NLS, strongly suggesting that NLS is required for the enhancement of Rel-dependent NF-kappa B transactivation. Furthermore, CgSIMPL mRNA is constitutively expressed in various tissues and is inducible at late stages of infection, supporting its regulatory function in innate immunity. Altogether, our studies reveal that SIMPL is reserved and co-evolved with NF-kappa B in chordate and mollusk, supporting its ancient origin and involvement in regulation of NF-kappa B signaling pathway. (C) 2014 Elsevier Ltd. All rights reserved.
部门归属[Zhang, Yang ; Li, Jun ; Yu, Feng ; Tong, Ying ; Zhang, Yuehuan ; Xiang, Zhiming ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
关键词Crassostrea Gigas Simpl Ancient Origin Rel/nf-kappa b Transcription Activity
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]
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收录类别sci
资助项目LMB
资助者National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095] ; National Basic Research Program of China [2010CB126404]; National Natural Science Foundation of China [41176150]; Joint Funds of NSFC-Guangdong of China [U1201215]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou of China [2013J2200095]
WOS记录号WOS:000341468500028
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10389
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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Zhang, Y,Li, J,Yu, F,et al. Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(1):239-244.
APA Zhang, Y.,Li, J.,Yu, F.,Tong, Y.,Zhang, YH.,...&carlzyu@scsio.ac.cn.(2014).Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity.FISH & SHELLFISH IMMUNOLOGY,40(1),239-244.
MLA Zhang, Y,et al."Identification and functional characterization of SIMPL in Crassostrea gigas reveals its ancient origin and involvement in the regulation of Rel/NF-kappa B transcription activity".FISH & SHELLFISH IMMUNOLOGY 40.1(2014):239-244.
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