Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology
Li, LL; Zhou, S; Ji, HS; Gao, R; Qin, QW; zhous@scsio.ac.cn; qinqw@scsio.ac.cn
2014
发表期刊CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
卷号32期号:5页码:1036-1045
摘要The industrially important organic compound 1,3-propanediol (1,3-PDO) is mainly used as a building block for the production of various polymers. In the present study, response surface methodology protocol was followed to determine and optimize fermentation conditions for the maximum production of 1,3-PDO using marine-derived Klebsiella pneumoniae HSL4. Four nutritional supplements together with three independent culture conditions were optimized as follows: 29.3 g/L glycerol, 8.0 g/L K-2 HPO4, 7.6 g/L (NH4)(2) SO4, 3.0 g/L KH2 PO4, pH 7.1, cultivation at 35A degrees C for 12 h. Under the optimal conditions, a maximum 1,3-PDO concentration of 14.5 g/L, a productivity of 1.21 g/(L center dot h) and a conversion of glycerol of 0.49 g/g were obtained. In comparison with the control conditions, fermentation under the optimized conditions achieved an increase of 38.8% in 1,3-PDO concentration, 39.0% in productivity and 25.7% in glycerol conversion in flask. This enhancement trend was further confirmed when the fermentation was conducted in a 5-L fermentor. The optimized fermentation conditions could be an important basis for developing lowcost, large-scale methods for industrial production of 1,3-PDO in the future.
部门归属[Li Lili ; Zhou Sheng ; Qin Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Li Lili] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Ji Huasong ; Gao Ren] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
关键词Klebsiella Pneumoniae Hsl4 1 Fermentation 3-propanediol (1 Response Surface Methodology (Rsm) 3-pdo) Optimization
学科领域Marine & Freshwater Biology ; Oceanography
资助者Scientific Research Project of Marine Public Welfare Industry of China [201205020-4]; Knowledge Innovation Project of Chinese Academy of Sciences [KSCX2-EW-G-12B]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002] ; Scientific Research Project of Marine Public Welfare Industry of China [201205020-4]; Knowledge Innovation Project of Chinese Academy of Sciences [KSCX2-EW-G-12B]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002] ; Scientific Research Project of Marine Public Welfare Industry of China [201205020-4]; Knowledge Innovation Project of Chinese Academy of Sciences [KSCX2-EW-G-12B]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002] ; Scientific Research Project of Marine Public Welfare Industry of China [201205020-4]; Knowledge Innovation Project of Chinese Academy of Sciences [KSCX2-EW-G-12B]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]
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收录类别sci
资助项目LMB
资助者Scientific Research Project of Marine Public Welfare Industry of China [201205020-4]; Knowledge Innovation Project of Chinese Academy of Sciences [KSCX2-EW-G-12B]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002] ; Scientific Research Project of Marine Public Welfare Industry of China [201205020-4]; Knowledge Innovation Project of Chinese Academy of Sciences [KSCX2-EW-G-12B]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002] ; Scientific Research Project of Marine Public Welfare Industry of China [201205020-4]; Knowledge Innovation Project of Chinese Academy of Sciences [KSCX2-EW-G-12B]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002] ; Scientific Research Project of Marine Public Welfare Industry of China [201205020-4]; Knowledge Innovation Project of Chinese Academy of Sciences [KSCX2-EW-G-12B]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]
WOS记录号WOS:000340879900009
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10390
专题中科院海洋生物资源可持续利用重点实验室
通讯作者zhous@scsio.ac.cn; qinqw@scsio.ac.cn
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GB/T 7714
Li, LL,Zhou, S,Ji, HS,et al. Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(5):1036-1045.
APA Li, LL.,Zhou, S.,Ji, HS.,Gao, R.,Qin, QW.,...&qinqw@scsio.ac.cn.(2014).Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(5),1036-1045.
MLA Li, LL,et al."Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.5(2014):1036-1045.
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