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学科主题: Marine & Freshwater Biology ; Oceanography
题名: Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology
作者: Li, LL ; Zhou, S ; Ji, HS ; Gao, R ; Qin, QW
通讯作者: zhous@scsio.ac.cn ; qinqw@scsio.ac.cn
关键词: 1,3-propanediol (1,3-PDO) ; Klebsiella pneumoniae HSL4 ; fermentation ; response surface methodology (RSM) ; optimization
刊名: CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
发表日期: 2014
卷: 32, 期:5, 页:1036-1045
收录类别: sci
部门归属: [Li Lili ; Zhou Sheng ; Qin Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Li Lili] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Ji Huasong ; Gao Ren] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
项目归属: LMB
资助者: Scientific Research Project of Marine Public Welfare Industry of China [201205020-4]; Knowledge Innovation Project of Chinese Academy of Sciences [KSCX2-EW-G-12B]; Administration of Ocean and Fisheries of Guangdong Province [GD2012-D01-002]
摘要: The industrially important organic compound 1,3-propanediol (1,3-PDO) is mainly used as a building block for the production of various polymers. In the present study, response surface methodology protocol was followed to determine and optimize fermentation conditions for the maximum production of 1,3-PDO using marine-derived Klebsiella pneumoniae HSL4. Four nutritional supplements together with three independent culture conditions were optimized as follows: 29.3 g/L glycerol, 8.0 g/L K-2 HPO4, 7.6 g/L (NH4)(2) SO4, 3.0 g/L KH2 PO4, pH 7.1, cultivation at 35A degrees C for 12 h. Under the optimal conditions, a maximum 1,3-PDO concentration of 14.5 g/L, a productivity of 1.21 g/(L center dot h) and a conversion of glycerol of 0.49 g/g were obtained. In comparison with the control conditions, fermentation under the optimized conditions achieved an increase of 38.8% in 1,3-PDO concentration, 39.0% in productivity and 25.7% in glycerol conversion in flask. This enhancement trend was further confirmed when the fermentation was conducted in a 5-L fermentor. The optimized fermentation conditions could be an important basis for developing lowcost, large-scale methods for industrial production of 1,3-PDO in the future.
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WOS记录号: WOS:000340879900009
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10390
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Li, LL; Zhou, S; Ji, HS; Gao, R; Qin, QW.Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology,CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(5):1036-1045
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