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Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis
Huang, YH; Huang, XH; Ouyang, ZL; Wei, SN; Guo, CY; Qin, QW; qinqw@scsio.ac.cn
2014
Source PublicationAQUACULTURE
ISSN0044-8486
Volume432Pages:265-272
AbstractWe developed and characterized a new marine fish cell line (ELGSN) from the snout of giant grouper, Epinephelus lanceolatus (Bloch). Two different phenotypes of cells, ELGSN(e), which consisted predominantly of epithelial-like cells, and ELGSN(f), which consisted predominantly of fibroblast-like cells, were isolated and cultured. Both ELGSN, and ELGSN(f) cells were subcultured for >80 passages and multiplied well in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum at 28 degrees C. Karyotyping analysis indicated that the modal chromosome numbers of ELGSN(e) and ELGSN(f) cells were 48 and 62, respectively. After transfection with EGFP-C1, bright green fluorescence was observed in these two cells and the transfection efficiency was 35% and 28%, respectively. Moreover, the two types of ELGSN cells both showed susceptibility to Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV), but not to soft-shelled turtle iridovirus (STIV) and spring viremia of carp virus (SVCV), indicated by the occurrence of severe cytopathic effect and increased viral titers. In SGIV-infected cells, paracrystalline arrays, different stages of virus particles and amorphous structures were observed under electron microscopy. In RGNNV-infected cells, numerous virus particles and circular virus-packed macropinosome-like structures were observed. All these data suggested that ELGSN cells could be used for effective virus propagation in vitro. In addition, we provided biochemical evidences that macropinocytosis was involved in RGNNV infection in ELGSN cells. Taken together, the new established ELGSN cell lines provided a useful tool for transgenic and genetic manipulation, as well as virus propagation and pathogenesis. (C) 2014 Elsevier B.V. All rights reserved.
Department[Huang, Youhua ; Huang, Xiaohong ; Ouyang, Zhengliang ; Wei, Shina ; Guo, Chuanyu ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
KeywordEpinephelus Lanceolatus Elgsn Snout Iridovirus Nodavirus Macropinocytosis
Subject AreaFisheries ; Marine & Freshwater Biology
Funding OrganizationNational Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014]
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Indexed Bysci
Funding ProjectLMB
Funding OrganizationNational Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014]
WOS IDWOS:000342247200034
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10399
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorqinqw@scsio.ac.cn
Recommended Citation
GB/T 7714
Huang, YH,Huang, XH,Ouyang, ZL,et al. Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis[J]. AQUACULTURE,2014,432:265-272.
APA Huang, YH.,Huang, XH.,Ouyang, ZL.,Wei, SN.,Guo, CY.,...&qinqw@scsio.ac.cn.(2014).Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis.AQUACULTURE,432,265-272.
MLA Huang, YH,et al."Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis".AQUACULTURE 432(2014):265-272.
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