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学科主题: Fisheries ; Marine & Freshwater Biology
题名: Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis
作者: Huang, YH ; Huang, XH ; Ouyang, ZL ; Wei, SN ; Guo, CY ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
关键词: Epinephelus lanceolatus ; ELGSN ; Snout ; Iridovirus ; Nodavirus ; Macropinocytosis
刊名: AQUACULTURE
发表日期: 2014
卷: 432, 页:265-272
收录类别: sci
部门归属: [Huang, Youhua ; Huang, Xiaohong ; Ouyang, Zhengliang ; Wei, Shina ; Guo, Chuanyu ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014]
摘要: We developed and characterized a new marine fish cell line (ELGSN) from the snout of giant grouper, Epinephelus lanceolatus (Bloch). Two different phenotypes of cells, ELGSN(e), which consisted predominantly of epithelial-like cells, and ELGSN(f), which consisted predominantly of fibroblast-like cells, were isolated and cultured. Both ELGSN, and ELGSN(f) cells were subcultured for >80 passages and multiplied well in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum at 28 degrees C. Karyotyping analysis indicated that the modal chromosome numbers of ELGSN(e) and ELGSN(f) cells were 48 and 62, respectively. After transfection with EGFP-C1, bright green fluorescence was observed in these two cells and the transfection efficiency was 35% and 28%, respectively. Moreover, the two types of ELGSN cells both showed susceptibility to Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV), but not to soft-shelled turtle iridovirus (STIV) and spring viremia of carp virus (SVCV), indicated by the occurrence of severe cytopathic effect and increased viral titers. In SGIV-infected cells, paracrystalline arrays, different stages of virus particles and amorphous structures were observed under electron microscopy. In RGNNV-infected cells, numerous virus particles and circular virus-packed macropinosome-like structures were observed. All these data suggested that ELGSN cells could be used for effective virus propagation in vitro. In addition, we provided biochemical evidences that macropinocytosis was involved in RGNNV infection in ELGSN cells. Taken together, the new established ELGSN cell lines provided a useful tool for transgenic and genetic manipulation, as well as virus propagation and pathogenesis. (C) 2014 Elsevier B.V. All rights reserved.
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WOS记录号: WOS:000342247200034
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10399
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Huang, YH; Huang, XH; Ouyang, ZL; Wei, SN; Guo, CY; Qin, QW.Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis,AQUACULTURE,2014,432():265-272
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