Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis
Huang, YH; Huang, XH; Ouyang, ZL; Wei, SN; Guo, CY; Qin, QW; qinqw@scsio.ac.cn
2014
发表期刊AQUACULTURE
ISSN0044-8486
卷号432页码:265-272
摘要We developed and characterized a new marine fish cell line (ELGSN) from the snout of giant grouper, Epinephelus lanceolatus (Bloch). Two different phenotypes of cells, ELGSN(e), which consisted predominantly of epithelial-like cells, and ELGSN(f), which consisted predominantly of fibroblast-like cells, were isolated and cultured. Both ELGSN, and ELGSN(f) cells were subcultured for >80 passages and multiplied well in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum at 28 degrees C. Karyotyping analysis indicated that the modal chromosome numbers of ELGSN(e) and ELGSN(f) cells were 48 and 62, respectively. After transfection with EGFP-C1, bright green fluorescence was observed in these two cells and the transfection efficiency was 35% and 28%, respectively. Moreover, the two types of ELGSN cells both showed susceptibility to Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV), but not to soft-shelled turtle iridovirus (STIV) and spring viremia of carp virus (SVCV), indicated by the occurrence of severe cytopathic effect and increased viral titers. In SGIV-infected cells, paracrystalline arrays, different stages of virus particles and amorphous structures were observed under electron microscopy. In RGNNV-infected cells, numerous virus particles and circular virus-packed macropinosome-like structures were observed. All these data suggested that ELGSN cells could be used for effective virus propagation in vitro. In addition, we provided biochemical evidences that macropinocytosis was involved in RGNNV infection in ELGSN cells. Taken together, the new established ELGSN cell lines provided a useful tool for transgenic and genetic manipulation, as well as virus propagation and pathogenesis. (C) 2014 Elsevier B.V. All rights reserved.
部门归属[Huang, Youhua ; Huang, Xiaohong ; Ouyang, Zhengliang ; Wei, Shina ; Guo, Chuanyu ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
关键词Epinephelus Lanceolatus Elgsn Snout Iridovirus Nodavirus Macropinocytosis
学科领域Fisheries ; Marine & Freshwater Biology
资助者National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014]
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收录类别sci
资助项目LMB
资助者National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014] ; National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [41206137, 30930070]; National High Technology Development Program of China (863) [SS2014AA091607]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201014]
WOS记录号WOS:000342247200034
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10399
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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Huang, YH,Huang, XH,Ouyang, ZL,et al. Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis[J]. AQUACULTURE,2014,432:265-272.
APA Huang, YH.,Huang, XH.,Ouyang, ZL.,Wei, SN.,Guo, CY.,...&qinqw@scsio.ac.cn.(2014).Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis.AQUACULTURE,432,265-272.
MLA Huang, YH,et al."Development of a new cell line from the snout of giant grouper, Epinephelus lanceolatus (Bloch), and its application in iridovirus and nodavirus pathogenesis".AQUACULTURE 432(2014):265-272.
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