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学科主题: Environmental Sciences & Ecology ; Marine & Freshwater Biology
题名: Molecular characterization of metallothionein from white shrimp, Litopenaeus vannamei and its expression response to salinity stress
作者: Wang, YH ; Zhao, Z ; Zhang, LP ; Hu, CQ ; Ren, CH ; Yuan, LH
通讯作者: cqhu@scsio.ac.cn
关键词: Gene expression ; Litopenaeus vannamei ; metallothionein ; salinity stress ; subcellular localization
刊名: MARINE BIOLOGY RESEARCH
发表日期: 2014
卷: 10, 期:7, 页:731-737
收录类别: sci
部门归属: [Wang, Yanhong ; Zhao, Zhe ; Zhang, Luping ; Hu, Chaoqu ; Ren, Chunhua ; Yuan, Lihong] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Appl Marine Biol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
资助者: National High Technology Research and Development Program of China (863 Program) [2012AA10A404-4]; Science & Technology Promoting Project from the Oceanic & Fishery Bureau of Guangdong Province [A201001A02, A201101B01, A201201B02]; Science and Technology Program of Guangdong Province [2012B091100270]
摘要: The metallothioneins (MTs) are conserved and multi-functional proteins which play crucial roles in the transportation of essential trace elements and the detoxification of harmful metallic elements. A novel MT homologue (accession no. JN707684) was isolated from the Litopenaeus vannamei hypersalinity subtractive library and used to carry out tissue-specific expression under varying salinity stress. The complete cDNA sequence of LvMT contains a 177 bp open reading frame encoding 58 amino acids, a 66 bp 5-UTR, and a 117 bp 3-UTR. The LvMT was expressed in the highest peak in the hepatopancreas under hyposalinity (3 g l(-1)) (19.43-fold, p < 0.01) and hypersalinity (40 g l(-1)) (22.3-fold, p < 0.01) stress for 6 h. A phylogenetic analysis revealed that LvMT is similar to other crustacean MT proteins. The LvMT was distributed in both the cytoplasm and the nucleus of HeLa and EPC cells.
原文出处: 查看原文
WOS记录号: WOS:000333879100007
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10403
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Wang, YH; Zhao, Z; Zhang, LP; Hu, CQ; Ren, CH; Yuan, LH.Molecular characterization of metallothionein from white shrimp, Litopenaeus vannamei and its expression response to salinity stress,MARINE BIOLOGY RESEARCH,2014,10(7):731-737
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