Molecular characterization of metallothionein from white shrimp, Litopenaeus vannamei and its expression response to salinity stress
Wang, YH; Zhao, Z; Zhang, LP; Hu, CQ; Ren, CH; Yuan, LH; cqhu@scsio.ac.cn
2014
发表期刊MARINE BIOLOGY RESEARCH
ISSN1745-1000
卷号10期号:7页码:731-737
摘要The metallothioneins (MTs) are conserved and multi-functional proteins which play crucial roles in the transportation of essential trace elements and the detoxification of harmful metallic elements. A novel MT homologue (accession no. JN707684) was isolated from the Litopenaeus vannamei hypersalinity subtractive library and used to carry out tissue-specific expression under varying salinity stress. The complete cDNA sequence of LvMT contains a 177 bp open reading frame encoding 58 amino acids, a 66 bp 5-UTR, and a 117 bp 3-UTR. The LvMT was expressed in the highest peak in the hepatopancreas under hyposalinity (3 g l(-1)) (19.43-fold, p < 0.01) and hypersalinity (40 g l(-1)) (22.3-fold, p < 0.01) stress for 6 h. A phylogenetic analysis revealed that LvMT is similar to other crustacean MT proteins. The LvMT was distributed in both the cytoplasm and the nucleus of HeLa and EPC cells.
部门归属[Wang, Yanhong ; Zhao, Zhe ; Zhang, Luping ; Hu, Chaoqu ; Ren, Chunhua ; Yuan, Lihong] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Appl Marine Biol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
关键词Gene Expression Litopenaeus Vannamei Metallothionein Salinity Stress Subcellular Localization
学科领域Environmental Sciences & Ecology ; Marine & Freshwater Biology
资助者National High Technology Research and Development Program of China (863 Program) [2012AA10A404-4]; Science & Technology Promoting Project from the Oceanic & Fishery Bureau of Guangdong Province [A201001A02, A201101B01, A201201B02]; Science and Technology Program of Guangdong Province [2012B091100270] ; National High Technology Research and Development Program of China (863 Program) [2012AA10A404-4]; Science & Technology Promoting Project from the Oceanic & Fishery Bureau of Guangdong Province [A201001A02, A201101B01, A201201B02]; Science and Technology Program of Guangdong Province [2012B091100270] ; National High Technology Research and Development Program of China (863 Program) [2012AA10A404-4]; Science & Technology Promoting Project from the Oceanic & Fishery Bureau of Guangdong Province [A201001A02, A201101B01, A201201B02]; Science and Technology Program of Guangdong Province [2012B091100270] ; National High Technology Research and Development Program of China (863 Program) [2012AA10A404-4]; Science & Technology Promoting Project from the Oceanic & Fishery Bureau of Guangdong Province [A201001A02, A201101B01, A201201B02]; Science and Technology Program of Guangdong Province [2012B091100270]
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收录类别sci
资助项目LMB
资助者National High Technology Research and Development Program of China (863 Program) [2012AA10A404-4]; Science & Technology Promoting Project from the Oceanic & Fishery Bureau of Guangdong Province [A201001A02, A201101B01, A201201B02]; Science and Technology Program of Guangdong Province [2012B091100270] ; National High Technology Research and Development Program of China (863 Program) [2012AA10A404-4]; Science & Technology Promoting Project from the Oceanic & Fishery Bureau of Guangdong Province [A201001A02, A201101B01, A201201B02]; Science and Technology Program of Guangdong Province [2012B091100270] ; National High Technology Research and Development Program of China (863 Program) [2012AA10A404-4]; Science & Technology Promoting Project from the Oceanic & Fishery Bureau of Guangdong Province [A201001A02, A201101B01, A201201B02]; Science and Technology Program of Guangdong Province [2012B091100270] ; National High Technology Research and Development Program of China (863 Program) [2012AA10A404-4]; Science & Technology Promoting Project from the Oceanic & Fishery Bureau of Guangdong Province [A201001A02, A201101B01, A201201B02]; Science and Technology Program of Guangdong Province [2012B091100270]
WOS记录号WOS:000333879100007
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10403
专题中科院海洋生物资源可持续利用重点实验室
通讯作者cqhu@scsio.ac.cn
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Wang, YH,Zhao, Z,Zhang, LP,et al. Molecular characterization of metallothionein from white shrimp, Litopenaeus vannamei and its expression response to salinity stress[J]. MARINE BIOLOGY RESEARCH,2014,10(7):731-737.
APA Wang, YH.,Zhao, Z.,Zhang, LP.,Hu, CQ.,Ren, CH.,...&cqhu@scsio.ac.cn.(2014).Molecular characterization of metallothionein from white shrimp, Litopenaeus vannamei and its expression response to salinity stress.MARINE BIOLOGY RESEARCH,10(7),731-737.
MLA Wang, YH,et al."Molecular characterization of metallothionein from white shrimp, Litopenaeus vannamei and its expression response to salinity stress".MARINE BIOLOGY RESEARCH 10.7(2014):731-737.
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