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学科主题: Virology
题名: Isolation and characterization of a new class of DNA aptamers specific binding to Singapore grouper iridovirus (SGIV) with antiviral activities
作者: Li, PF ; Yan, Y ; Wei, SN ; Wei, JG ; Gao, R ; Huang, XH ; Huang, YH ; Jiang, GH ; Qin, QW
通讯作者: jgh982@bnu.edu.cn ; qinqw@scsio.ac.cn
关键词: DNA aptamers ; SELEX, SGIV ; Grouper ; Antiviral agents
刊名: VIRUS RESEARCH
发表日期: 2014
卷: 188, 页:146-154
收录类别: sci
部门归属: [Li, Pengfei ; Yan, Yang ; Wei, Shina ; Wei, Jingguang ; Huang, Xiaohong ; Huang, Youhua ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Jiang, Guohua] Beijing Normal Univ, Analyt & Testing Ctr, Beijing 100875, Peoples R China ; [Li, Pengfei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Gao, Ren] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China (973) [2012CB114402]; National Natural Science Foundation of China [31330082]
摘要: The Singapore grouper iridovirus (SGIV), a member of the genus Ranavirus, is a major viral pathogen that has caused heavy economic losses to the grouper aquaculture industry in China and Southeast Asia. No efficient method of controlling SGIV outbreaks is currently available. Systematic evolution of ligands by exponential enrichment (SELEX) is now widely used for the in vitro selection of artificial ssDNA or RNA ligands, known as aptamers, which bind to targets through their stable three-dimensional structures. In our current study, we generated ssDNA aptamers against the SGIV, and evaluated their ability to block SGIV infection in cultured fish cells and cultured fish in vivo. The anti-SGIV DNA aptamers, LMB-761, LMB-764, LMB-748, LMB-439, LMB-755, and LMB-767, were selected from a pool of oligonucleotides randomly generated using a SELEX iterative method. The analysis of the secondary structure of the aptamers revealed that they all formed similar stem-loop structures. Electrophoretic mobility shift assays showed that the aptamers bound SGIV specifically, as evidenced by a lack cross-reactivity with the soft shell turtle iridovirus. The aptamers produced no cytotoxic effects in cultured grouper spleen cells (GS). Assessment of cytopathic effects (CPE) and viral titer assays showed that LMB-761, LMB-764, LMB-748, LMB-755, and LMB-767 significantly inhibited SGIV infection in GS cells. The in vivo experiments showed that LMB-761 and LMB-764 reduced SGIV-related mortality, and no negative effects were observed in orange-spotted grouper, Epinephelus coioides, indicating that these DNA aptamers may be suitable antiviral candidates for controlling SGIV infections in fish reared in marine aquaculture facilities. (C) 2014 Elsevier B.V. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000339128300019
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10404
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Li, PF; Yan, Y; Wei, SN; Wei, JG; Gao, R; Huang, XH; Huang, YH; Jiang, GH; Qin, QW.Isolation and characterization of a new class of DNA aptamers specific binding to Singapore grouper iridovirus (SGIV) with antiviral activities,VIRUS RESEARCH,2014,188():146-154
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