A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata
Zhao, M; He, MX; Huang, XD; Wang, Q; hmx@scsio.ac.cn
2014
发表期刊PLOS ONE
ISSN1932-6203
卷号9期号:8页码:-e103830
摘要We reported pearl oyster Pinctada fucata cDNA and genomic characterization of a new homeobox-containing protein, PfMSX. The PfMSX gene encodes a transcription factor that was localized to the nucleus. Analyses of PfMSX mRNA in tissues and developmental stages showed high expressions in mantle or D-shaped larvae. In electrophoretic mobility shift assays (EMSAs) PfMSX binded to MSX consensus binding sites in the 59 flanking region of the Pif promoter. In co-transfection experiment PfMSX transactivated reporter constructs containing Pif promoter sequences, and mutation of the MSX-binding sites attenuated transactivation. A knockdown experiment using PfMSX dsRNA showed decreased Pif mRNA and unregular crystallization of the nacreous layer using scanning electron microscopy. Our results suggested that PfMSX was a conserved homeodomain transcription factor gene, which can activate Pif gene expression through MSX binding site, and was then involved in the mineralization process in pearl oyster Pinctada fucata. Our data provided important clues about mechanisms regulating biomineralization in pearl oyster.
部门归属[Zhao, Mi ; He, Maoxian ; Huang, Xiande ; Wang, Qi] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China ; [Zhao, Mi ; Wang, Qi] Univ Chinese Acad Sci, Beijing, Peoples R China
学科领域Science & Technology - Other Topics
资助者National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21]
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收录类别sci
资助项目LMB
资助者National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21]
WOS记录号WOS:000339995100034
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10405
专题中科院海洋生物资源可持续利用重点实验室
通讯作者hmx@scsio.ac.cn
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GB/T 7714
Zhao, M,He, MX,Huang, XD,et al. A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata[J]. PLOS ONE,2014,9(8):-e103830.
APA Zhao, M,He, MX,Huang, XD,Wang, Q,&hmx@scsio.ac.cn.(2014).A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata.PLOS ONE,9(8),-e103830.
MLA Zhao, M,et al."A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata".PLOS ONE 9.8(2014):-e103830.
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