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学科主题: Science & Technology - Other Topics
题名: A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata
作者: Zhao, M ; He, MX ; Huang, XD ; Wang, Q
通讯作者: hmx@scsio.ac.cn
刊名: PLOS ONE
发表日期: 2014
卷: 9, 期:8, 页:-e103830
收录类别: sci
部门归属: [Zhao, Mi ; He, Maoxian ; Huang, Xiande ; Wang, Qi] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China ; [Zhao, Mi ; Wang, Qi] Univ Chinese Acad Sci, Beijing, Peoples R China
项目归属: LMB
资助者: National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21]
摘要: We reported pearl oyster Pinctada fucata cDNA and genomic characterization of a new homeobox-containing protein, PfMSX. The PfMSX gene encodes a transcription factor that was localized to the nucleus. Analyses of PfMSX mRNA in tissues and developmental stages showed high expressions in mantle or D-shaped larvae. In electrophoretic mobility shift assays (EMSAs) PfMSX binded to MSX consensus binding sites in the 59 flanking region of the Pif promoter. In co-transfection experiment PfMSX transactivated reporter constructs containing Pif promoter sequences, and mutation of the MSX-binding sites attenuated transactivation. A knockdown experiment using PfMSX dsRNA showed decreased Pif mRNA and unregular crystallization of the nacreous layer using scanning electron microscopy. Our results suggested that PfMSX was a conserved homeodomain transcription factor gene, which can activate Pif gene expression through MSX binding site, and was then involved in the mineralization process in pearl oyster Pinctada fucata. Our data provided important clues about mechanisms regulating biomineralization in pearl oyster.
原文出处: 查看原文
WOS记录号: WOS:000339995100034
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10405
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Zhao, M; He, MX; Huang, XD; Wang, Q.A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata,PLOS ONE,2014,9(8):-e103830
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