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A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata
Zhao, M; He, MX; Huang, XD; Wang, Q; hmx@scsio.ac.cn
2014
Source PublicationPLOS ONE
ISSN1932-6203
Volume9Issue:8Pages:-e103830
AbstractWe reported pearl oyster Pinctada fucata cDNA and genomic characterization of a new homeobox-containing protein, PfMSX. The PfMSX gene encodes a transcription factor that was localized to the nucleus. Analyses of PfMSX mRNA in tissues and developmental stages showed high expressions in mantle or D-shaped larvae. In electrophoretic mobility shift assays (EMSAs) PfMSX binded to MSX consensus binding sites in the 59 flanking region of the Pif promoter. In co-transfection experiment PfMSX transactivated reporter constructs containing Pif promoter sequences, and mutation of the MSX-binding sites attenuated transactivation. A knockdown experiment using PfMSX dsRNA showed decreased Pif mRNA and unregular crystallization of the nacreous layer using scanning electron microscopy. Our results suggested that PfMSX was a conserved homeodomain transcription factor gene, which can activate Pif gene expression through MSX binding site, and was then involved in the mineralization process in pearl oyster Pinctada fucata. Our data provided important clues about mechanisms regulating biomineralization in pearl oyster.
Department[Zhao, Mi ; He, Maoxian ; Huang, Xiande ; Wang, Qi] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China ; [Zhao, Mi ; Wang, Qi] Univ Chinese Acad Sci, Beijing, Peoples R China
Subject AreaScience & Technology - Other Topics
Funding OrganizationNational Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21]
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Indexed Bysci
Funding ProjectLMB
Funding OrganizationNational Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21] ; National Natural Science Foundation of China [41376159]; National Science and technology program of China [2012AA10A410]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21]
WOS IDWOS:000339995100034
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10405
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorhmx@scsio.ac.cn
Recommended Citation
GB/T 7714
Zhao, M,He, MX,Huang, XD,et al. A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata[J]. PLOS ONE,2014,9(8):-e103830.
APA Zhao, M,He, MX,Huang, XD,Wang, Q,&hmx@scsio.ac.cn.(2014).A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata.PLOS ONE,9(8),-e103830.
MLA Zhao, M,et al."A Homeodomain Transcription Factor Gene, PfMSX, Activates Expression of Pif Gene in the Pearl Oyster Pinctada fucata".PLOS ONE 9.8(2014):-e103830.
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