Molecular characterization and analysis of a putative 5-HT receptor involved in reproduction process of the pearl oyster Pinctada fucata
Wang, Q; He, MX; hmx@scsio.ac.cn
2014
发表期刊GENERAL AND COMPARATIVE ENDOCRINOLOGY
ISSN0016-6480
卷号204页码:71-79
摘要5-HT (5-hydroxytryptamine; serotonin) has been linked to a variety of biological roles including gonad maturation and sequential spawning in bivalve molluscs. To gain a better understanding of the effects of 5-HT on developmental regulation in the pearl oyster Pinctada fucata, the isolation, cloning, and expression of the 5-HT receptor was investigated in this study. A full-length cDNA (2541 bp) encoding a putative 5-HT receptor (5-HTpf) of 471 amino acids was isolated from the ovary of the pearl oyster. It shared 71% and 51% homology, respectively, with the Crassostrea gigas 5-HT receptor and the Aplysia californica 5-HT1ap. The 5-HTpf sequence possessed the typical characteristics of seven transmembrane domains and a long third inner loop. Phylogenetic analysis also indicated that 5-HTpf was classified into the 5-HT1 subtype together with other invertebrate 5-HT1 receptors. Quantitative RT-PCR showed that 5-HTpf is widely expressed in all tissues tested, is involved in the gametogenesis cycle, embryonic and larval development stages, and expression is induced by E-2 in ovarian tissues. These results suggest that 5-HTpf is involved in the reproductive process, specifically in the induction of oocyte maturation and spawning of P. fucata. (C) 2014 Elsevier Inc. All rights reserved.
部门归属[Wang, Qi ; He, Maoxian] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Marine Bioresource Sustainable Utiliz, Guangzhou 510301, Guangdong, Peoples R China ; [Wang, Qi] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
关键词5-ht Receptor Oocyte Maturation Spawning Pinctada Fucata
学科领域Endocrinology & Metabolism
资助者Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05, A201301A03]; National Science and Technology Program of China [2012AA10A410] ; Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05, A201301A03]; National Science and Technology Program of China [2012AA10A410] ; Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05, A201301A03]; National Science and Technology Program of China [2012AA10A410] ; Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05, A201301A03]; National Science and Technology Program of China [2012AA10A410]
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收录类别sci
资助项目LMB
资助者Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05, A201301A03]; National Science and Technology Program of China [2012AA10A410] ; Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05, A201301A03]; National Science and Technology Program of China [2012AA10A410] ; Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05, A201301A03]; National Science and Technology Program of China [2012AA10A410] ; Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05, A201301A03]; National Science and Technology Program of China [2012AA10A410]
WOS记录号WOS:000341616200009
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10409
专题中科院海洋生物资源可持续利用重点实验室
通讯作者hmx@scsio.ac.cn
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Wang, Q,He, MX,hmx@scsio.ac.cn. Molecular characterization and analysis of a putative 5-HT receptor involved in reproduction process of the pearl oyster Pinctada fucata[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2014,204:71-79.
APA Wang, Q,He, MX,&hmx@scsio.ac.cn.(2014).Molecular characterization and analysis of a putative 5-HT receptor involved in reproduction process of the pearl oyster Pinctada fucata.GENERAL AND COMPARATIVE ENDOCRINOLOGY,204,71-79.
MLA Wang, Q,et al."Molecular characterization and analysis of a putative 5-HT receptor involved in reproduction process of the pearl oyster Pinctada fucata".GENERAL AND COMPARATIVE ENDOCRINOLOGY 204(2014):71-79.
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