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学科主题: Endocrinology & Metabolism
题名: Molecular characterization and analysis of a putative 5-HT receptor involved in reproduction process of the pearl oyster Pinctada fucata
作者: Wang, Q ; He, MX
通讯作者: hmx@scsio.ac.cn
关键词: 5-HT receptor ; Oocyte maturation ; Spawning ; Pinctada fucata
刊名: GENERAL AND COMPARATIVE ENDOCRINOLOGY
发表日期: 2014
卷: 204, 页:71-79
收录类别: sci
部门归属: [Wang, Qi ; He, Maoxian] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Marine Bioresource Sustainable Utiliz, Guangzhou 510301, Guangdong, Peoples R China ; [Wang, Qi] Univ Chinese Acad Sci, Beijing 100039, Peoples R China
项目归属: LMB
资助者: Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05, A201301A03]; National Science and Technology Program of China [2012AA10A410]
摘要: 5-HT (5-hydroxytryptamine; serotonin) has been linked to a variety of biological roles including gonad maturation and sequential spawning in bivalve molluscs. To gain a better understanding of the effects of 5-HT on developmental regulation in the pearl oyster Pinctada fucata, the isolation, cloning, and expression of the 5-HT receptor was investigated in this study. A full-length cDNA (2541 bp) encoding a putative 5-HT receptor (5-HTpf) of 471 amino acids was isolated from the ovary of the pearl oyster. It shared 71% and 51% homology, respectively, with the Crassostrea gigas 5-HT receptor and the Aplysia californica 5-HT1ap. The 5-HTpf sequence possessed the typical characteristics of seven transmembrane domains and a long third inner loop. Phylogenetic analysis also indicated that 5-HTpf was classified into the 5-HT1 subtype together with other invertebrate 5-HT1 receptors. Quantitative RT-PCR showed that 5-HTpf is widely expressed in all tissues tested, is involved in the gametogenesis cycle, embryonic and larval development stages, and expression is induced by E-2 in ovarian tissues. These results suggest that 5-HTpf is involved in the reproductive process, specifically in the induction of oocyte maturation and spawning of P. fucata. (C) 2014 Elsevier Inc. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000341616200009
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10409
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Wang, Q; He, MX.Molecular characterization and analysis of a putative 5-HT receptor involved in reproduction process of the pearl oyster Pinctada fucata,GENERAL AND COMPARATIVE ENDOCRINOLOGY,2014,204():71-79
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