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学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: The first molluscan acute phase serum amyloid A (A-SAA) identified from oyster Crassostrea hongkongensis: Molecular cloning and functional characterization
作者: Qu, FF ; Xiang, ZM ; Yu, ZN
通讯作者: carlzyu@scsio.ac.cn
关键词: Crassostrea hongkongensis ; Acute phase serum amyloid A (A-SAA) ; Innate immunity ; NF-kappa B
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2014
卷: 39, 期:2, 页:145-151
收录类别: sci
部门归属: [Qu, Fufa ; Xiang, Zhiming ; Yu, Ziniu] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Qu, Fufa] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
项目归属: LMB
资助者: National Natural Science Foundation of China [31202021]; National Science Foundation of China [41176150]; National Basic Research Program of China [2010CB126404]
摘要: Serum amyloid A (SAA), a major evolutionarily conserved acute-phase protein, participates in many biological processes in eukaryotic cells, including innate immunity. However, little information regarding the relationship between SAA and innate immunity in mollusks is currently available. In this report, the first bivalve SAA (referred to as ChSAA) gene was identified and characterized from the Hong Kong oyster Crassostrea hongkongensis. Its full-length cDNA is 623 bp, including a 5'-UTR of 147 bp, a 3'-UTR of 56 bp containing a poly(A) tail and an open reading frame (ORF) of 420 bp that encodes a polypeptide of 139 amino acids. The predicted amino acid sequence of ChSAA comprises characteristic motifs of the SAA family, including a typical signal peptide and a conserved SAA domain. Comparison and phylogenetic analyses suggested that ChSAA shares a high identity to known acute-phase SAA proteins (A-SAAs). In addition, quantitative real-time PCR analysis revealed that ChSAA is constitutively expressed in all tissues examined, with the highest expression level in the mantle, and that its expression was acutely and significantly up-regulated in hemocytes following challenge by Vibrio alginolyticus (G(-)), Staphylococcus haemolyticus (G(+)) or Saccharomyces cerevisiae (fungus). Furthermore, over-expression of ChSAA via transfection with a ChSAA expression vector led to significantly increased NF-kappa B activity in HEK293T cells. These results suggest that ChSAA is likely to constitute a member of the A-SAA family involved in anti-pathogen responses in C hongkongensis. (C) 2014 Elsevier Ltd. All rights reserved.
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WOS记录号: WOS:000339773000004
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10415
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Qu, FF; Xiang, ZM; Yu, ZN.The first molluscan acute phase serum amyloid A (A-SAA) identified from oyster Crassostrea hongkongensis: Molecular cloning and functional characterization,FISH & SHELLFISH IMMUNOLOGY,2014,39(2):145-151
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