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学科主题: Biotechnology & Applied Microbiology
题名: Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library
作者: Mai, ZM ; Su, HF ; Yang, J ; Huang, SJ ; Zhang, S
通讯作者: maizhimao@163.com ; suhongfei17@126.com ; yangjian@scsio.ac.cn ; huangsijun@scsio.ac.cn ; zhsimd@scsio.ac.cn
关键词: Cellulase ; Mangrove ; Metagenomic library ; Organic solvent-resistant ; Salt-tolerant
刊名: BIOTECHNOLOGY LETTERS
发表日期: 2014
卷: 36, 期:8, 页:1701-1709
收录类别: sci
部门归属: [Mai, Zhimao ; Su, Hongfei ; Yang, Jian ; Huang, Sijun ; Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China ; [Mai, Zhimao ; Su, Hongfei ; Zhang, Si] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China ; [Zhang, Si] Chinese Acad Sci, South China Sea Inst Ocean, Guangzhou 530104, Guangdong, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China (973 Program) [2010CB 833801]; National Natural Science Foundation of China [41230962]
摘要: A novel endoglucanase gene, mgcel44, was isolated from a mangrove soil metagenomic library by functional-based screening. It encodes a 648-aa peptide with a catalytic domain of glycosyl hydrolase family 44. The deduced amino acid sequence of mgcel44 shares less than 50 % identity with endoglucanases in GenBank database. mgcel44 was cloned and overexpressed in Escherichia coli. The recombinant enzyme, MgCel44, has a molecular mass of 70.8 kDa as determined by SDS-PAGE. Its optimal pH and temperature for activity were 6 and 45 A degrees C, respectively. It was highly active at 25-45 A degrees C and pH 5-8. Its activity was enhanced in 0.5 M NaCl by > 1.6-fold and stable up to 1.5 M NaCl. MgCel44 was resistant to several organic solvents and had high activity at 15 % (v/v) solvent after incubating for 24 h at 25 A degrees C.
原文出处: 查看原文
WOS记录号: WOS:000339004900018
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10417
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Mai, ZM; Su, HF; Yang, J; Huang, SJ; Zhang, S.Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library,BIOTECHNOLOGY LETTERS,2014,36(8):1701-1709
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