Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library
Mai, ZM; Su, HF; Yang, J; Huang, SJ; Zhang, S; maizhimao@163.com; suhongfei17@126.com; yangjian@scsio.ac.cn; huangsijun@scsio.ac.cn; zhsimd@scsio.ac.cn
2014
发表期刊BIOTECHNOLOGY LETTERS
ISSN0141-5492
卷号36期号:8页码:1701-1709
摘要A novel endoglucanase gene, mgcel44, was isolated from a mangrove soil metagenomic library by functional-based screening. It encodes a 648-aa peptide with a catalytic domain of glycosyl hydrolase family 44. The deduced amino acid sequence of mgcel44 shares less than 50 % identity with endoglucanases in GenBank database. mgcel44 was cloned and overexpressed in Escherichia coli. The recombinant enzyme, MgCel44, has a molecular mass of 70.8 kDa as determined by SDS-PAGE. Its optimal pH and temperature for activity were 6 and 45 A degrees C, respectively. It was highly active at 25-45 A degrees C and pH 5-8. Its activity was enhanced in 0.5 M NaCl by > 1.6-fold and stable up to 1.5 M NaCl. MgCel44 was resistant to several organic solvents and had high activity at 15 % (v/v) solvent after incubating for 24 h at 25 A degrees C.
部门归属[Mai, Zhimao ; Su, Hongfei ; Yang, Jian ; Huang, Sijun ; Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China ; [Mai, Zhimao ; Su, Hongfei ; Zhang, Si] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China ; [Zhang, Si] Chinese Acad Sci, South China Sea Inst Ocean, Guangzhou 530104, Guangdong, Peoples R China
关键词Cellulase Mangrove Metagenomic Library Organic Solvent-resistant Salt-tolerant
学科领域Biotechnology & Applied Microbiology
资助者National Basic Research Program of China (973 Program) [2010CB 833801]; National Natural Science Foundation of China [41230962] ; National Basic Research Program of China (973 Program) [2010CB 833801]; National Natural Science Foundation of China [41230962] ; National Basic Research Program of China (973 Program) [2010CB 833801]; National Natural Science Foundation of China [41230962] ; National Basic Research Program of China (973 Program) [2010CB 833801]; National Natural Science Foundation of China [41230962]
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收录类别sci
资助项目LMB
资助者National Basic Research Program of China (973 Program) [2010CB 833801]; National Natural Science Foundation of China [41230962] ; National Basic Research Program of China (973 Program) [2010CB 833801]; National Natural Science Foundation of China [41230962] ; National Basic Research Program of China (973 Program) [2010CB 833801]; National Natural Science Foundation of China [41230962] ; National Basic Research Program of China (973 Program) [2010CB 833801]; National Natural Science Foundation of China [41230962]
WOS记录号WOS:000339004900018
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10417
专题中科院海洋生物资源可持续利用重点实验室
通讯作者maizhimao@163.com; suhongfei17@126.com; yangjian@scsio.ac.cn; huangsijun@scsio.ac.cn; zhsimd@scsio.ac.cn
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GB/T 7714
Mai, ZM,Su, HF,Yang, J,et al. Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library[J]. BIOTECHNOLOGY LETTERS,2014,36(8):1701-1709.
APA Mai, ZM.,Su, HF.,Yang, J.,Huang, SJ.,Zhang, S.,...&zhsimd@scsio.ac.cn.(2014).Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library.BIOTECHNOLOGY LETTERS,36(8),1701-1709.
MLA Mai, ZM,et al."Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library".BIOTECHNOLOGY LETTERS 36.8(2014):1701-1709.
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