SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
The asymmetric membrane structure of erythrocytes from Crucian carp studied by atomic force microscopy
Tian, YM; Cai, MJ; Zhao, WD; Wang, SW; Qin, QW; Wang, HD; hdwang@ciac.ac.cn
2014
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume59Issue:21Pages:2582-2587
AbstractCell membranes play a key role in cellular activities. Fish erythrocytes, prototype of the nucleated erythrocytes of lower vertebrates, are predominantly oval, biconvex discs with elliptical nucleus and much larger in size than human erythrocytes. In attempts to disclose the correlation of membrane structure between fish and human erythrocytes, we used in situ atomic force microscope (AFM) combined with single-molecule force spectroscopy to study the membrane structure of Crucian carp erythrocytes under quasi-native conditions. Our results revealed the asymmetric distribution of proteins in Crucian carp erythrocyte membrane: the outer leaflet of membrane is rather smooth without any proteins, whereas the inner leaflet of membrane is very rough with dense proteins. The asymmetry of fish erythrocyte membrane structure fits well with the semi-mosaic model of human erythrocyte membrane structure. This similarity of membrane structure between human and fish erythrocytes extends the semi-mosaic model of erythrocytes membrane structure to a wider range of species.
Department[Tian, Yong-Mei ; Cai, Ming-Jun ; Zhao, Wei-Dong ; Wang, Hong-Da] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanal Chem, Changchun 130022, Peoples R China ; [Tian, Yong-Mei ; Zhao, Wei-Dong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Wang, Shao-Wen ; Qin, Qi-Wei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
KeywordAtomic Force Microscope Crucian Carp Erythrocyte Membrane Structure Asymmetry
Subject AreaScience & Technology - Other Topics
Funding OrganizationMinistry of Science and Technology of China [2011CB933600]; National Natural Science Foundation of China [21373200, 31330082, 21203177] ; Ministry of Science and Technology of China [2011CB933600]; National Natural Science Foundation of China [21373200, 31330082, 21203177] ; Ministry of Science and Technology of China [2011CB933600]; National Natural Science Foundation of China [21373200, 31330082, 21203177] ; Ministry of Science and Technology of China [2011CB933600]; National Natural Science Foundation of China [21373200, 31330082, 21203177]
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Indexed Bysci
Funding ProjectLMB
Funding OrganizationMinistry of Science and Technology of China [2011CB933600]; National Natural Science Foundation of China [21373200, 31330082, 21203177] ; Ministry of Science and Technology of China [2011CB933600]; National Natural Science Foundation of China [21373200, 31330082, 21203177] ; Ministry of Science and Technology of China [2011CB933600]; National Natural Science Foundation of China [21373200, 31330082, 21203177] ; Ministry of Science and Technology of China [2011CB933600]; National Natural Science Foundation of China [21373200, 31330082, 21203177]
WOS IDWOS:000338127100012
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10423
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorhdwang@ciac.ac.cn
Recommended Citation
GB/T 7714
Tian, YM,Cai, MJ,Zhao, WD,et al. The asymmetric membrane structure of erythrocytes from Crucian carp studied by atomic force microscopy[J]. CHINESE SCIENCE BULLETIN,2014,59(21):2582-2587.
APA Tian, YM.,Cai, MJ.,Zhao, WD.,Wang, SW.,Qin, QW.,...&hdwang@ciac.ac.cn.(2014).The asymmetric membrane structure of erythrocytes from Crucian carp studied by atomic force microscopy.CHINESE SCIENCE BULLETIN,59(21),2582-2587.
MLA Tian, YM,et al."The asymmetric membrane structure of erythrocytes from Crucian carp studied by atomic force microscopy".CHINESE SCIENCE BULLETIN 59.21(2014):2582-2587.
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