Isolation and characterization of tumor necrosis factor receptor-associated factor 6 (TRAF6) from grouper, Epinephelus tauvina
Wei, JG; Guo, ML; Gao, P; Ji, HS; Li, PF; Yan, Y; Qin, QW; qinqw@scsio.ac.cn
2014
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号39期号:1页码:61-68
摘要Tumor necrosis factor receptor-associated factor 6 (TRAF6) is one of the key adapter molecules in Toll-like receptor signal transduction that triggers downstream cascades involved in innate immunity. In the present study, a TRAF6 (named as Et-TRAF6) was identified from the marine fish grouper, Epinephelus tauvina by RACE PCR. The full-length cDNA of Et-TRAF6 comprised 1949 bp with a 1713 bp open reading frame (ORF) that encodes a putative protein of 570 amino acids. Similar to most TRAF6s, Et-TRAF6 includes one N-terminal RING domain (78aa-116aa), two zinc fingers of TRAF-type (159aa-210aa and 212aa-269aa), one coiled-coil region (370aa-394aa), and one conserved C-terminal meprin and TRAF homology (MATH) domain (401aa-526aa). Quantitative real-time PCR analysis revealed that Et-TRAF6 mRNA is expressed in all tested tissues, with the predominant expression in the stomach and intestine. The expression of Et-TRAF6 was up-regulated in the liver after challenge with Lipoteichoic acid (LTA), Peptidoglycan (PGN), Zymosan, polyinosine polycytidylic acid [Poly(I:C)] and Polydeoxyadenylic acid -Polythymidylic acid sodium salt [Poly(dA:dT)]. The expression of Et-TRAF6 was also up-regulated in the liver after infection with Vibrio alginolyticus, Singapore grouper iridovirus (SGIV) and grouper nervous necrosis virus (GNNV). Recombinant Et-TRAF6 (rEt-TRAF6) was expressed in Escherichia BL21 (DE3) and purified for mouse anti-Et-TRAF6 serum preparation. Intracellular localization revealed that Et-TRAF6 is distributed in both cytoplasm and nucleus, and predominantly in the cytoplasm. These results together indicated that Et-TRAF6 might be involved in immune responses toward bacterial and virus challenges. (C) 2014 Elsevier Ltd. All rights reserved.
部门归属[Wei, Jingguang ; Guo, Minglan ; Li, Pengfei ; Yan, Yang ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Gao, Pin ; Ji, Huasong] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
关键词Epinephelus Tauvina Traf6 Sgiv Gnnv Intracellular Localization
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者National Natural Science Foundation of China [31202022]; National High Technology Development Program of China (863) [2012AA092201]; National Sparking Plan Project of China [2013GA780001]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201203]; Science and Technology Program of Guangdong [2012A020603018] ; National Natural Science Foundation of China [31202022]; National High Technology Development Program of China (863) [2012AA092201]; National Sparking Plan Project of China [2013GA780001]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201203]; Science and Technology Program of Guangdong [2012A020603018] ; National Natural Science Foundation of China [31202022]; National High Technology Development Program of China (863) [2012AA092201]; National Sparking Plan Project of China [2013GA780001]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201203]; Science and Technology Program of Guangdong [2012A020603018] ; National Natural Science Foundation of China [31202022]; National High Technology Development Program of China (863) [2012AA092201]; National Sparking Plan Project of China [2013GA780001]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201203]; Science and Technology Program of Guangdong [2012A020603018]
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收录类别sci
资助项目LMB
资助者National Natural Science Foundation of China [31202022]; National High Technology Development Program of China (863) [2012AA092201]; National Sparking Plan Project of China [2013GA780001]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201203]; Science and Technology Program of Guangdong [2012A020603018] ; National Natural Science Foundation of China [31202022]; National High Technology Development Program of China (863) [2012AA092201]; National Sparking Plan Project of China [2013GA780001]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201203]; Science and Technology Program of Guangdong [2012A020603018] ; National Natural Science Foundation of China [31202022]; National High Technology Development Program of China (863) [2012AA092201]; National Sparking Plan Project of China [2013GA780001]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201203]; Science and Technology Program of Guangdong [2012A020603018] ; National Natural Science Foundation of China [31202022]; National High Technology Development Program of China (863) [2012AA092201]; National Sparking Plan Project of China [2013GA780001]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201203]; Science and Technology Program of Guangdong [2012A020603018]
WOS记录号WOS:000338613400008
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10424
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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Wei, JG,Guo, ML,Gao, P,et al. Isolation and characterization of tumor necrosis factor receptor-associated factor 6 (TRAF6) from grouper, Epinephelus tauvina[J]. FISH & SHELLFISH IMMUNOLOGY,2014,39(1):61-68.
APA Wei, JG.,Guo, ML.,Gao, P.,Ji, HS.,Li, PF.,...&qinqw@scsio.ac.cn.(2014).Isolation and characterization of tumor necrosis factor receptor-associated factor 6 (TRAF6) from grouper, Epinephelus tauvina.FISH & SHELLFISH IMMUNOLOGY,39(1),61-68.
MLA Wei, JG,et al."Isolation and characterization of tumor necrosis factor receptor-associated factor 6 (TRAF6) from grouper, Epinephelus tauvina".FISH & SHELLFISH IMMUNOLOGY 39.1(2014):61-68.
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