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学科主题: Oceanography
题名: Effect of temperature on gene expression in the pearl oyster Pinctada fucata
作者: Liu, WG ; Huang, XD ; Lin, JS ; He, MX
通讯作者: hmx@scsio.ac.cn
关键词: seawater temperature ; heat shock protein ; gene expression pattern ; Pinctada fucata
刊名: JOURNAL OF OCEAN UNIVERSITY OF CHINA
发表日期: 2014
卷: 13, 期:3, 页:509-515
收录类别: sci
部门归属: [Liu Wenguang ; Huang Xiande ; Lin Jianshi ; He Maoxian] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine BiorResources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
资助者: National Natural Science Foundation of China [41006090]; Joint Program of NSFC-Guangdong [U0831001]; Funds of Knowledge Innovation Program of Chinese Academy of Sciences [ZCX2-EW-Q21]
摘要: In this study, we examined the effect of elevated temperature on the expression patterns of genes, i.e., nacrein, irr, n16, n19, and hsp70 in the pearl oyster Pinctada fucata. The experiment was carried out at 4 temperatures, i.e., 20A degrees C (ambient, control), 24, 28A degrees C, and 32A degrees C. The expression levels of target genes in P. fucata were assayed at 0, 6, 24, 48, and 96 h via real-time polymerase chain reaction. Results showed that the expression levels of nacrein and irr had no significant variations among different time points below 28A degrees C, but significantly increased over time at 32A degrees C. The expression levels of n16 and n19 did not change markedly at 20A degrees C. The former increased significantly at 6 h and 24 h while the latter substantially decreased during 6-96 h at 24, 28 and 32A degrees C. Among different temperatures, the level of n16 was significantly lower at 20A degrees C than at other temperatures during 6-96 h, and the level of n19 significantly varied among different temperatures at 48 h and 96 h. The expression level of hsp70 was significantly higher at 32A degrees C than at 20, 24 and 28A degrees C at 24 h. These results demonstrated that elevated temperature impacted the physiological processes of P. fucata and potentially influenced its adaptability to thermal stress.
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WOS记录号: WOS:000335672600023
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10436
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Liu, WG; Huang, XD; Lin, JS; He, MX.Effect of temperature on gene expression in the pearl oyster Pinctada fucata,JOURNAL OF OCEAN UNIVERSITY OF CHINA,2014,13(3):509-515
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