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学科主题: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
题名: Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis
作者: Xie, YC ; Li, QL ; Song, YX ; Ma, JY ; Ju, JH
通讯作者: jju@scsio.ac.cn
关键词: antibiotics ; biosynthesis ; carbon-sulfur bonds ; griseoviridin ; P450 monooxygenases ; viridogrisein
刊名: CHEMBIOCHEM
发表日期: 2014
卷: 15, 期:8, 页:1183-1189
收录类别: sci
部门归属: [Xie, Yunchang ; Li, Qinglian ; Song, Yongxiang ; Ma, Junying ; Ju, Jianhua] Chinese Acad Sci, Guangdong Key Lab Marine Mat Med, CAS Key Lab Trop Marine Bioresources & Ecol, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
资助者: NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12]
摘要: Griseoviridin (GV) is an A-type streptogramin antibiotic displaying antimicrobial activity and acting synergistically with viridogrisein (VG). Bioinformatic analyses reveal SgvP as the sole cytochrome P450 enzyme in the GV/VG gene cluster. To explore the role of SgvP in the GV/VG pathway, we inactivated the sgvP gene. The resulting sgvP mutant generated two new products: GV-1 and GV-2, both lacking the CS bridge. In trans complementation of the sgvP gene into the sgvP mutant strain partially restores GV production. Feeding [1-13C]-labeled cysteine to the wild-type strain led to enrichment of C-7 in the GV scaffold, thus verifying that the CS bond in GV is formed through direct coupling of the free SH group provided by the side chain of cysteine. The above results highlight the significance of SgvP in CS bond formation in griseoviridin biosynthesis.
原文出处: 查看原文
WOS记录号: WOS:000337582200017
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10438
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Xie, YC; Li, QL; Song, YX; Ma, JY; Ju, JH.Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis,CHEMBIOCHEM,2014,15(8):1183-1189
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