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Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis
Xie, YC; Li, QL; Song, YX; Ma, JY; Ju, JH; jju@scsio.ac.cn
2014
Source PublicationCHEMBIOCHEM
ISSN1439-4227
Volume15Issue:8Pages:1183-1189
AbstractGriseoviridin (GV) is an A-type streptogramin antibiotic displaying antimicrobial activity and acting synergistically with viridogrisein (VG). Bioinformatic analyses reveal SgvP as the sole cytochrome P450 enzyme in the GV/VG gene cluster. To explore the role of SgvP in the GV/VG pathway, we inactivated the sgvP gene. The resulting sgvP mutant generated two new products: GV-1 and GV-2, both lacking the CS bridge. In trans complementation of the sgvP gene into the sgvP mutant strain partially restores GV production. Feeding [1-13C]-labeled cysteine to the wild-type strain led to enrichment of C-7 in the GV scaffold, thus verifying that the CS bond in GV is formed through direct coupling of the free SH group provided by the side chain of cysteine. The above results highlight the significance of SgvP in CS bond formation in griseoviridin biosynthesis.
Department[Xie, Yunchang ; Li, Qinglian ; Song, Yongxiang ; Ma, Junying ; Ju, Jianhua] Chinese Acad Sci, Guangdong Key Lab Marine Mat Med, CAS Key Lab Trop Marine Bioresources & Ecol, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China
KeywordAntibiotics Biosynthesis Carbon-sulfur Bonds Griseoviridin P450 Monooxygenases Viridogrisein
Subject AreaBiochemistry & Molecular Biology ; Pharmacology & Pharmacy
Funding OrganizationNSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12]
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Indexed Bysci
Funding ProjectLMB
Funding OrganizationNSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12]
WOS IDWOS:000337582200017
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10438
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorjju@scsio.ac.cn
Recommended Citation
GB/T 7714
Xie, YC,Li, QL,Song, YX,et al. Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis[J]. CHEMBIOCHEM,2014,15(8):1183-1189.
APA Xie, YC,Li, QL,Song, YX,Ma, JY,Ju, JH,&jju@scsio.ac.cn.(2014).Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis.CHEMBIOCHEM,15(8),1183-1189.
MLA Xie, YC,et al."Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis".CHEMBIOCHEM 15.8(2014):1183-1189.
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