Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis
Xie, YC; Li, QL; Song, YX; Ma, JY; Ju, JH; jju@scsio.ac.cn
2014
发表期刊CHEMBIOCHEM
ISSN1439-4227
卷号15期号:8页码:1183-1189
摘要Griseoviridin (GV) is an A-type streptogramin antibiotic displaying antimicrobial activity and acting synergistically with viridogrisein (VG). Bioinformatic analyses reveal SgvP as the sole cytochrome P450 enzyme in the GV/VG gene cluster. To explore the role of SgvP in the GV/VG pathway, we inactivated the sgvP gene. The resulting sgvP mutant generated two new products: GV-1 and GV-2, both lacking the CS bridge. In trans complementation of the sgvP gene into the sgvP mutant strain partially restores GV production. Feeding [1-13C]-labeled cysteine to the wild-type strain led to enrichment of C-7 in the GV scaffold, thus verifying that the CS bond in GV is formed through direct coupling of the free SH group provided by the side chain of cysteine. The above results highlight the significance of SgvP in CS bond formation in griseoviridin biosynthesis.
部门归属[Xie, Yunchang ; Li, Qinglian ; Song, Yongxiang ; Ma, Junying ; Ju, Jianhua] Chinese Acad Sci, Guangdong Key Lab Marine Mat Med, CAS Key Lab Trop Marine Bioresources & Ecol, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China
关键词Antibiotics Biosynthesis Carbon-sulfur Bonds Griseoviridin P450 Monooxygenases Viridogrisein
学科领域Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
资助者NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12]
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收录类别sci
资助项目LMB
资助者NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12] ; NSFC [21172231]; MOST [2010CB833805, 2012AA092104]; Knowledge Innovation Program of CAS [KSCX2-EW-G-12]
WOS记录号WOS:000337582200017
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10438
专题中科院海洋生物资源可持续利用重点实验室
通讯作者jju@scsio.ac.cn
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Xie, YC,Li, QL,Song, YX,et al. Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis[J]. CHEMBIOCHEM,2014,15(8):1183-1189.
APA Xie, YC,Li, QL,Song, YX,Ma, JY,Ju, JH,&jju@scsio.ac.cn.(2014).Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis.CHEMBIOCHEM,15(8),1183-1189.
MLA Xie, YC,et al."Involvement of SgvP in Carbon-Sulfur Bond Formation during Griseoviridin Biosynthesis".CHEMBIOCHEM 15.8(2014):1183-1189.
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