Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses
Yan, Y; Cui, HC; Guo, CY; Wei, JG; Huang, YH; Li, LL; Qin, QW; qinqw@scsio.ac.cn
2014
发表期刊JOURNAL OF GENERAL VIROLOGY
ISSN0022-1317
卷号95页码:1144-1155
摘要Semaphorins are a large, phylogenetically conserved family of proteins that are involved in a wide range of biological processes including axonal steering, organogenesis, neoplastic transformation, as well as immune responses. In this study, a novel semaphorin homologue gene belonging to the Singapore grouper iridovirus (SGIV), ORF155R (termed SGIV-sema), was cloned and characterized. The coding region of SGIV-sema is 1728 bp in length, encoding a predicted protein with 575 aa. SGIV-sema contains a similar to 370 aa N-terminal Sema domain, a conserved plexin-semaphorin-integrin (PSI) domain, and an immunoglobulin (Ig)-like domain near the C terminus. SGIV-sema is an early gene product during viral infection and predominantly distributed in the cytoplasm with a speckled and clubbed pattern of appearance. Functionally, SGIV-sema could promote viral replication during SGIV infection in vitro, with no effect on the proliferation of host cells. Intriguingly, ectopically expressed SGIV-sema could alter the cytoskeletal structure of fish cells, characterized by a circumferential ring of microtubules near the nucleus and a disrupted microfilament organization. Furthermore, SGIV-sema was able to attenuate the cellular immune response, as demonstrated by decreased expression of inflammation/immune-related genes such as IL-8, IL-15, TNF-alpha and mediator of IRF3 activation (MITA), in SGIV-sema-expressing cells before and after SGIV infection. Ultimately, our study identified a novel, functional SGIV gene that could regulate cytoskeletal structure, immune responses and facilitate viral replication.
部门归属[Yan, Yang ; Guo, Chuanyu ; Wei, Jingguang ; Huang, Youhua ; Li, Lili ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Cui, Huachun] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA ; [Guo, Chuanyu ; Li, Lili] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
学科领域Biotechnology & Applied Microbiology ; Virology
资助者National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [41206137, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201116, KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [41206137, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201116, KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [41206137, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201116, KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [41206137, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201116, KZCX2-EW-Q213]
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收录类别sci
资助项目LMB
资助者National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [41206137, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201116, KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [41206137, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201116, KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [41206137, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201116, KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [41206137, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201116, KZCX2-EW-Q213]
WOS记录号WOS:000338177900016
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10441
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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Yan, Y,Cui, HC,Guo, CY,et al. Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses[J]. JOURNAL OF GENERAL VIROLOGY,2014,95:1144-1155.
APA Yan, Y.,Cui, HC.,Guo, CY.,Wei, JG.,Huang, YH.,...&qinqw@scsio.ac.cn.(2014).Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses.JOURNAL OF GENERAL VIROLOGY,95,1144-1155.
MLA Yan, Y,et al."Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses".JOURNAL OF GENERAL VIROLOGY 95(2014):1144-1155.
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