中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Biotechnology & Applied Microbiology ; Virology
题名: Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses
作者: Yan, Y ; Cui, HC ; Guo, CY ; Wei, JG ; Huang, YH ; Li, LL ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
刊名: JOURNAL OF GENERAL VIROLOGY
发表日期: 2014
卷: 95, 页:1144-1155
收录类别: sci
部门归属: [Yan, Yang ; Guo, Chuanyu ; Wei, Jingguang ; Huang, Youhua ; Li, Lili ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Cui, Huachun] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA ; [Guo, Chuanyu ; Li, Lili] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [41206137, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [SQ201116, KZCX2-EW-Q213]
摘要: Semaphorins are a large, phylogenetically conserved family of proteins that are involved in a wide range of biological processes including axonal steering, organogenesis, neoplastic transformation, as well as immune responses. In this study, a novel semaphorin homologue gene belonging to the Singapore grouper iridovirus (SGIV), ORF155R (termed SGIV-sema), was cloned and characterized. The coding region of SGIV-sema is 1728 bp in length, encoding a predicted protein with 575 aa. SGIV-sema contains a similar to 370 aa N-terminal Sema domain, a conserved plexin-semaphorin-integrin (PSI) domain, and an immunoglobulin (Ig)-like domain near the C terminus. SGIV-sema is an early gene product during viral infection and predominantly distributed in the cytoplasm with a speckled and clubbed pattern of appearance. Functionally, SGIV-sema could promote viral replication during SGIV infection in vitro, with no effect on the proliferation of host cells. Intriguingly, ectopically expressed SGIV-sema could alter the cytoskeletal structure of fish cells, characterized by a circumferential ring of microtubules near the nucleus and a disrupted microfilament organization. Furthermore, SGIV-sema was able to attenuate the cellular immune response, as demonstrated by decreased expression of inflammation/immune-related genes such as IL-8, IL-15, TNF-alpha and mediator of IRF3 activation (MITA), in SGIV-sema-expressing cells before and after SGIV infection. Ultimately, our study identified a novel, functional SGIV gene that could regulate cytoskeletal structure, immune responses and facilitate viral replication.
原文出处: 查看原文
WOS记录号: WOS:000338177900016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10441
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Yan, Y; Cui, HC; Guo, CY; Wei, JG; Huang, YH; Li, LL; Qin, QW.Singapore grouper iridovirus-encoded semaphorin homologue (SGIV-sema) contributes to viral replication, cytoskeleton reorganization and inhibition of cellular immune responses,JOURNAL OF GENERAL VIROLOGY,2014,95():1144-1155
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yan, Y]'s Articles
[Cui, HC]'s Articles
[Guo, CY]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yan, Y]‘s Articles
[Cui, HC]‘s Articles
[Guo, CY]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace