Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro
Liu, JX; Luo, MQ; Xia, M; Wu, Q; Long, SM; Hu, YH; Gao, GC; Yao, XL; He, MA; Su, HX; Luo, XM; Yao, SZ; liujxiu@mail.sysu.edu.cn; zhanglmq@sina.com; xiameng25@163.com; wuqi@medmail.com.cn; ivylsm@163.com; yaohuahu225@aliyun.com; gaogcjx@163.com; liliyao71@163.com; gz87335487@163.net; Huanxingsu@umac.mo; xiongmingluo@scsio.ac.cn; yaoshuzh@mail.sysu.edu.cn
2014
发表期刊MARINE DRUGS
ISSN1660-3397
卷号12期号:5页码:2790-2801
摘要Angiogenesis is the formation of blood vessels from pre-existing vasculature. Excessive or uncontrolled angiogenesis is a major contributor to many pathological conditions whereas inhibition of aberrant angiogenesis is beneficial to patients with pathological angiogenesis. Catunaregin is a core of novel marine compound isolated from mangrove associate. The potential anti-angiogenesis of catunaregin was investigated in human umbilical vein endothelial cells (HUVECs) and zebrafish. HUVECs were treated with different concentrations of catunaregin in the presence or absence of VEGF. The angiogenic phenotypes including cell invasion cell migration and tube formation were evaluated following catunaregin treatment in HUVECs. The possible involvement of AKT, eNOS and ERK1/2 in catunaregin-induced anti-angiogenesis was explored using Western blotting. The anti-angiogenesis of catunaregin was further tested in the zebrafish embryo neovascularization and caudal fin regeneration assays. We found that catunaregin dose-dependently inhibited angiogenesis in both HUVECs and zebrafish embryo neovascularization and zebrafish caudal fin regeneration assays. In addition, catunaregin significantly decreased the phosphorylation of Akt and eNOS, but not the phosphorylation of ERK1/2. The present work demonstrates that catunaregin exerts the anti-angiogenic activity at least in part through the regulation of the Akt and eNOS signaling pathways.
部门归属[Liu, Jun-Xiu ; Xia, Meng ; He, Mian ; Yao, Shu-Zhong] Sun Yat Sen Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China ; [Luo, Min-Qi] Sun Yat Sen Univ, Dept Clin Lab, Affiliated Hosp 3, Guangzhou 510630, Guangdong, Peoples R China ; [Wu, Qi ; Long, Si-Mei ; Yao, Xiao-Li] Sun Yat Sen Univ, Guangdong Key Lab Diag & Treatment Major Neurol D, Dept Neurol, Natl Key Clin Dept,Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China ; [Wu, Qi ; Long, Si-Mei ; Yao, Xiao-Li] Sun Yat Sen Univ, Key Discipline Neurol, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China ; [Hu, Yaohua ; Su, Huanxing] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macao 999078, Peoples R China ; [Gao, Guang-Chun] Jiaxing Univ, Coll Med, Jiaxing 314001, Peoples R China ; [Luo, Xiong-Ming] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
关键词Anti-angiogenesis Catunaregin Vegf Zebrafish Huvecs
学科领域Pharmacology & Pharmacy
资助者National Key Clinical Department; National Key Discipline; Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases; National Natural Science Foundation of China [81371255, 81100936]; Doctoral Program of Higher Education of China [20110171110058]; Young Scientists Fund of the National Natural Science Foundation of China [41006091]; Guangdong Technological grant [2010B050700024, 2011B050400031, 2012B031800107]; Natural Science Foundation of Guangdong Province [S2011010004860]; Sun Yat-sen University [2007010]; university of Macau [MYRG122 (Y1-L3)-ICMS12-SHX] ; National Key Clinical Department; National Key Discipline; Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases; National Natural Science Foundation of China [81371255, 81100936]; Doctoral Program of Higher Education of China [20110171110058]; Young Scientists Fund of the National Natural Science Foundation of China [41006091]; Guangdong Technological grant [2010B050700024, 2011B050400031, 2012B031800107]; Natural Science Foundation of Guangdong Province [S2011010004860]; Sun Yat-sen University [2007010]; university of Macau [MYRG122 (Y1-L3)-ICMS12-SHX] ; National Key Clinical Department; National Key Discipline; Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases; National Natural Science Foundation of China [81371255, 81100936]; Doctoral Program of Higher Education of China [20110171110058]; Young Scientists Fund of the National Natural Science Foundation of China [41006091]; Guangdong Technological grant [2010B050700024, 2011B050400031, 2012B031800107]; Natural Science Foundation of Guangdong Province [S2011010004860]; Sun Yat-sen University [2007010]; university of Macau [MYRG122 (Y1-L3)-ICMS12-SHX] ; National Key Clinical Department; National Key Discipline; Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases; National Natural Science Foundation of China [81371255, 81100936]; Doctoral Program of Higher Education of China [20110171110058]; Young Scientists Fund of the National Natural Science Foundation of China [41006091]; Guangdong Technological grant [2010B050700024, 2011B050400031, 2012B031800107]; Natural Science Foundation of Guangdong Province [S2011010004860]; Sun Yat-sen University [2007010]; university of Macau [MYRG122 (Y1-L3)-ICMS12-SHX]
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资助项目LMB
资助者National Key Clinical Department; National Key Discipline; Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases; National Natural Science Foundation of China [81371255, 81100936]; Doctoral Program of Higher Education of China [20110171110058]; Young Scientists Fund of the National Natural Science Foundation of China [41006091]; Guangdong Technological grant [2010B050700024, 2011B050400031, 2012B031800107]; Natural Science Foundation of Guangdong Province [S2011010004860]; Sun Yat-sen University [2007010]; university of Macau [MYRG122 (Y1-L3)-ICMS12-SHX] ; National Key Clinical Department; National Key Discipline; Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases; National Natural Science Foundation of China [81371255, 81100936]; Doctoral Program of Higher Education of China [20110171110058]; Young Scientists Fund of the National Natural Science Foundation of China [41006091]; Guangdong Technological grant [2010B050700024, 2011B050400031, 2012B031800107]; Natural Science Foundation of Guangdong Province [S2011010004860]; Sun Yat-sen University [2007010]; university of Macau [MYRG122 (Y1-L3)-ICMS12-SHX] ; National Key Clinical Department; National Key Discipline; Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases; National Natural Science Foundation of China [81371255, 81100936]; Doctoral Program of Higher Education of China [20110171110058]; Young Scientists Fund of the National Natural Science Foundation of China [41006091]; Guangdong Technological grant [2010B050700024, 2011B050400031, 2012B031800107]; Natural Science Foundation of Guangdong Province [S2011010004860]; Sun Yat-sen University [2007010]; university of Macau [MYRG122 (Y1-L3)-ICMS12-SHX] ; National Key Clinical Department; National Key Discipline; Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases; National Natural Science Foundation of China [81371255, 81100936]; Doctoral Program of Higher Education of China [20110171110058]; Young Scientists Fund of the National Natural Science Foundation of China [41006091]; Guangdong Technological grant [2010B050700024, 2011B050400031, 2012B031800107]; Natural Science Foundation of Guangdong Province [S2011010004860]; Sun Yat-sen University [2007010]; university of Macau [MYRG122 (Y1-L3)-ICMS12-SHX]
WOS记录号WOS:000337160500024
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10442
专题中科院海洋生物资源可持续利用重点实验室
通讯作者liujxiu@mail.sysu.edu.cn; zhanglmq@sina.com; xiameng25@163.com; wuqi@medmail.com.cn; ivylsm@163.com; yaohuahu225@aliyun.com; gaogcjx@163.com; liliyao71@163.com; gz87335487@163.net; Huanxingsu@umac.mo; xiongmingluo@scsio.ac.cn; yaoshuzh@mail.sysu.edu.cn
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Liu, JX,Luo, MQ,Xia, M,et al. Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro[J]. MARINE DRUGS,2014,12(5):2790-2801.
APA Liu, JX.,Luo, MQ.,Xia, M.,Wu, Q.,Long, SM.,...&yaoshuzh@mail.sysu.edu.cn.(2014).Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro.MARINE DRUGS,12(5),2790-2801.
MLA Liu, JX,et al."Marine Compound Catunaregin Inhibits Angiogenesis through the Modulation of Phosphorylation of Akt and eNOS in vivo and in vitro".MARINE DRUGS 12.5(2014):2790-2801.
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