中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Molecular cloning and characterization of two types of I kappa B alpha orthologues in orange-spotted grouper, Epinephelus coioides
作者: Gao, R ; Huang, YH ; Huang, XH ; Guan, LY ; Wei, SN ; Zhou, YC ; Qin, QW
通讯作者: zychnu@163.com ; qinqw@scsio.ac.cn
关键词: Epinephelus coioides ; EcI kappa B alpha A ; EcI kappa B alpha B ; NF-kappa B ; SGIV
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2014
卷: 38, 期:1, 页:101-110
收录类别: sci
部门归属: [Gao, Ren ; Zhou, Yongcan ; Qin, Qiwei] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China ; [Gao, Ren ; Huang, Youhua ; Huang, Xiaohong ; Guan, Liya ; Wei, Shina ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China (973) [2012CB114402, 2012CB114404]; Natural Science Foundation of China [31172437, 31372566, 31330082]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213]
摘要: Inhibitors of kappa B (I kappa Bs) are the members of primary regulators of NF-kappa B, which can inhibit NF-kappa B activity by blocking the NF-kappa B in an inactive state in the cytoplasm. In this study, two types of IKB alpha orthologues (EcI kappa B alpha A and EcI kappa B alpha B) from orange-spotted grouper, Epinephelus coioides, were cloned and characterized. EcI kappa B alpha A and EcIKB alpha B encoded putative proteins containing 308 and 318 amino acids, which shared 59% and 53% identity to I kappa B alpha A and I kappa B alpha B of Danio rerio, respectively. Amino acid sequence alignment showed that both EcI kappa B alpha A and EcI kappa B alpha B contained a conserved degradation motif DSGLDS in the N-terminal region and a PEST sequence in the C-terminal region. In addition, EcI kappa B alpha A and EcI kappa B alpha B contained 5 and 6 ankcyrin repeats, respectively. The genomic DNA of EcI kappa B alpha A and EcI kappa B alpha B consisted of 6 exons and 5 introns. Both of their transcripts were widely distributed in different tissues, and the expression levels were different in response to various stimuli, including lipopolysaccharide (LPS), Vibrio alginolyticus and Singapore grouper iridovirus (SGIV). Dual-luciferase reporter assay suggested that both EcI kappa B alpha A and EcI kappa B alpha B were able to inhibit Ecc-Rel and Ecp65 induced NF-kappa B promoter activity in grouper spleen (GS) cells. Subcellular localization analysis showed that EcI kappa B alpha B was present predominantly in the cytoplasm, while EclicB alpha A was distributed throughout both the nucleus and the cytoplasm. Furthermore, overexpression of EcI kappa B alpha A and EcI kappa B alpha B in GS cells inhibited the viral gene transcriptions of MCP, ORF019 and ORF162 of SGIV. Taken together, our findings suggested that both EcI kappa B alpha A and EcI kappa B alpha B were involved in grouper innate immunity against virus. (C) 2014 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000336353700014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10444
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item:

There are no files associated with this item.


Recommended Citation:
Gao, R; Huang, YH; Huang, XH; Guan, LY; Wei, SN; Zhou, YC; Qin, QW.Molecular cloning and characterization of two types of I kappa B alpha orthologues in orange-spotted grouper, Epinephelus coioides,FISH & SHELLFISH IMMUNOLOGY,2014,38(1):101-110
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gao, R]'s Articles
[Huang, YH]'s Articles
[Huang, XH]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gao, R]‘s Articles
[Huang, YH]‘s Articles
[Huang, XH]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace