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学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis
作者: Xiang, ZM ; Qu, FF ; Qi, L ; Ying, T ; Li, J ; Shu, X ; Yu, ZN
通讯作者: carlzyu@scsio.ac.cn
关键词: Crassostrea hongkongensis ; Immunity ; Apoptosis ; ChDFFA
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2014
卷: 38, 期:1, 页:119-126
收录类别: sci
部门归属: [Xiang, Zhiming ; Qu, Fufa ; Ying, Tong ; Li, Jun ; Shu, Xiao ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Qu, Fufa ; Li, Jun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Qi, Lin] Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
项目归属: LMB
资助者: National Science Foundation of China [U1201215, 31202021]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095]
摘要: Apoptosis plays an important pathophysiological role in the homeostasis of immune systems. DNA fragmentation factors (DFFs) have been shown to be essential for DNA fragmentation, and the resultant DNA fragments follow a laddering pattern during apoptosis in vertebrates. In invertebrates, the functions of the DFF orthologs are not well characterized; therefore, we cloned and characterized a bivalve DFFA ortholog from the Hong Kong oyster Crassostrea hongkongensis (designated ChDFFA). The full-length cDNA of ChDFFA is 1186 bp in length and encodes a putative protein of 200 amino acids that contains an N-terminal CAD domain and a DFF-C domain at its C-terminus. Real-time RT-PCR results showed that ChDFFA is ubiquitously expressed in several tissues, and its highest expression is in gill. Following a 3- to 48-h challenge by microbial infection, the expression of ChDFFA increased in hemocytes. Using fluorescence microscopy, ChDFFA was localized in nuclei when exogenously expressed in HeLa cells. In addition, overexpression of ChDFFA inhibited the transcriptional activities of p53/p21-Luc reporter genes in HEK293T cells. These results suggest that ChDFFA may be involved in immune response reactions in the Hong Kong oyster C hongkongensis. (C) 2014 Elsevier Ltd. All rights reserved.
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WOS记录号: WOS:000336353700016
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10445
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Xiang, ZM; Qu, FF; Qi, L; Ying, T; Li, J; Shu, X; Yu, ZN.Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis,FISH & SHELLFISH IMMUNOLOGY,2014,38(1):119-126
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