Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis
Xiang, ZM; Qu, FF; Qi, L; Ying, T; Li, J; Shu, X; Yu, ZN; carlzyu@scsio.ac.cn
2014
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号38期号:1页码:119-126
摘要Apoptosis plays an important pathophysiological role in the homeostasis of immune systems. DNA fragmentation factors (DFFs) have been shown to be essential for DNA fragmentation, and the resultant DNA fragments follow a laddering pattern during apoptosis in vertebrates. In invertebrates, the functions of the DFF orthologs are not well characterized; therefore, we cloned and characterized a bivalve DFFA ortholog from the Hong Kong oyster Crassostrea hongkongensis (designated ChDFFA). The full-length cDNA of ChDFFA is 1186 bp in length and encodes a putative protein of 200 amino acids that contains an N-terminal CAD domain and a DFF-C domain at its C-terminus. Real-time RT-PCR results showed that ChDFFA is ubiquitously expressed in several tissues, and its highest expression is in gill. Following a 3- to 48-h challenge by microbial infection, the expression of ChDFFA increased in hemocytes. Using fluorescence microscopy, ChDFFA was localized in nuclei when exogenously expressed in HeLa cells. In addition, overexpression of ChDFFA inhibited the transcriptional activities of p53/p21-Luc reporter genes in HEK293T cells. These results suggest that ChDFFA may be involved in immune response reactions in the Hong Kong oyster C hongkongensis. (C) 2014 Elsevier Ltd. All rights reserved.
部门归属[Xiang, Zhiming ; Qu, Fufa ; Ying, Tong ; Li, Jun ; Shu, Xiao ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Qu, Fufa ; Li, Jun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Qi, Lin] Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
关键词Crassostrea Hongkongensis Immunity Apoptosis Chdffa
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者National Science Foundation of China [U1201215, 31202021]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [U1201215, 31202021]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [U1201215, 31202021]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [U1201215, 31202021]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095]
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收录类别sci
资助项目LMB
资助者National Science Foundation of China [U1201215, 31202021]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [U1201215, 31202021]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [U1201215, 31202021]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [U1201215, 31202021]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095]
WOS记录号WOS:000336353700016
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10445
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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Xiang, ZM,Qu, FF,Qi, L,et al. Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2014,38(1):119-126.
APA Xiang, ZM.,Qu, FF.,Qi, L.,Ying, T.,Li, J.,...&carlzyu@scsio.ac.cn.(2014).Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,38(1),119-126.
MLA Xiang, ZM,et al."Cloning and characterization of an apoptosis-related DNA fragmentation factor (DFF) from oyster, Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 38.1(2014):119-126.
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