中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus
作者: Ren, CH ; Chen, T ; Jiang, X ; Wang, YH ; Hu, CQ
通讯作者: rosemary166@sina.com ; chan1010@scsio.ac.cn ; jiangxiao4367@126.com ; yhwang@scsio.ac.cn ; hucq@scsio.ac.cn
关键词: Ferritin ; Stichopus monotuberculatus ; Anti-oxidation activity ; Iron binding capacity ; Innate immune defense
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2014
卷: 38, 期:1, 页:265-274
收录类别: sci
部门归属: [Hu, Chaoqun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 510301, Guangdong, Peoples R China ; Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China ; Chinese Acad Sci, Chinese Acad Sci LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
资助者: Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272]
摘要: Ferritin is one of the major non-harm iron storage proteins that found in most cell types of animals, plants and microorganisms. In this study, a ferritin subunit named StmFer was identified from the sea cucumber (Stichopus monotuberculatus) and characterized functionally. The full-length cDNA of StmFer is 1184 bp in size with a 5'-untranslated region (UTR) of 131 bp, a 3'-UTR of 531 bp and an open reading frame of 522 bp that encoding a protein of 173 amino acids with a deduced molecular weight of 19.95 kDa. StmFer possesses both the ferroxidase center of vertebrate ferritin heavy subunit and iron nucleation sites of vertebrate ferritin light subunit. For the gene structure, StmFer contains only three exons separated by two introns. Higher levels of mRNA expression were noticed in intestine and coelomocyte of S. monotuberculans by northern blot analysis. In in vitro experiments performed in coelomocytes, transcriptional expression of StmFer showed the strongest response to polyriboinosinic polyribocytidylic acid [Poly (I:C)] (9.08 fold up-regulation), followed by lipopolysaccharides (LPS), ferrous chloride (FeCl2) and inactivated bacteria (Vibrio alginolyticus) (7.84, 7.41 and 4.90 fold up-regulation, respectively) after 3 h post-challenge. In addition, the anti-oxidation activity and iron binding capacity of recombinant ferritin protein were demonstrated in this study. As a whole, our study suggested that the ferritin from sea cucumber may play critical roles not only in the cellular and organismic iron homeostasis, but also in the innate immune defense. (C) 2014 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000336353700033
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10446
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
1-s2.0-S1050464814000928-main.pdf(2287KB)----开放获取View Download

Recommended Citation:
Ren, CH; Chen, T; Jiang, X; Wang, YH; Hu, CQ.Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus,FISH & SHELLFISH IMMUNOLOGY,2014,38(1):265-274
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ren, CH]'s Articles
[Chen, T]'s Articles
[Jiang, X]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ren, CH]‘s Articles
[Chen, T]‘s Articles
[Jiang, X]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 1-s2.0-S1050464814000928-main.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace