Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus
Ren, CH; Chen, T; Jiang, X; Wang, YH; Hu, CQ; rosemary166@sina.com; chan1010@scsio.ac.cn; jiangxiao4367@126.com; yhwang@scsio.ac.cn; hucq@scsio.ac.cn
2014
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号38期号:1页码:265-274
摘要Ferritin is one of the major non-harm iron storage proteins that found in most cell types of animals, plants and microorganisms. In this study, a ferritin subunit named StmFer was identified from the sea cucumber (Stichopus monotuberculatus) and characterized functionally. The full-length cDNA of StmFer is 1184 bp in size with a 5'-untranslated region (UTR) of 131 bp, a 3'-UTR of 531 bp and an open reading frame of 522 bp that encoding a protein of 173 amino acids with a deduced molecular weight of 19.95 kDa. StmFer possesses both the ferroxidase center of vertebrate ferritin heavy subunit and iron nucleation sites of vertebrate ferritin light subunit. For the gene structure, StmFer contains only three exons separated by two introns. Higher levels of mRNA expression were noticed in intestine and coelomocyte of S. monotuberculans by northern blot analysis. In in vitro experiments performed in coelomocytes, transcriptional expression of StmFer showed the strongest response to polyriboinosinic polyribocytidylic acid [Poly (I:C)] (9.08 fold up-regulation), followed by lipopolysaccharides (LPS), ferrous chloride (FeCl2) and inactivated bacteria (Vibrio alginolyticus) (7.84, 7.41 and 4.90 fold up-regulation, respectively) after 3 h post-challenge. In addition, the anti-oxidation activity and iron binding capacity of recombinant ferritin protein were demonstrated in this study. As a whole, our study suggested that the ferritin from sea cucumber may play critical roles not only in the cellular and organismic iron homeostasis, but also in the innate immune defense. (C) 2014 Elsevier Ltd. All rights reserved.
部门归属[Hu, Chaoqun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 510301, Guangdong, Peoples R China ; Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China ; Chinese Acad Sci, Chinese Acad Sci LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
关键词Ferritin Stichopus Monotuberculatus Anti-oxidation Activity Iron Binding Capacity Innate Immune Defense
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272]
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收录类别sci
资助项目LMB
资助者Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272]
WOS记录号WOS:000336353700033
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被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10446
专题中科院海洋生物资源可持续利用重点实验室
通讯作者rosemary166@sina.com; chan1010@scsio.ac.cn; jiangxiao4367@126.com; yhwang@scsio.ac.cn; hucq@scsio.ac.cn
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Ren, CH,Chen, T,Jiang, X,et al. Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2014,38(1):265-274.
APA Ren, CH.,Chen, T.,Jiang, X.,Wang, YH.,Hu, CQ.,...&hucq@scsio.ac.cn.(2014).Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus.FISH & SHELLFISH IMMUNOLOGY,38(1),265-274.
MLA Ren, CH,et al."Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus".FISH & SHELLFISH IMMUNOLOGY 38.1(2014):265-274.
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