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Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus
Ren, CH; Chen, T; Jiang, X; Wang, YH; Hu, CQ; rosemary166@sina.com; chan1010@scsio.ac.cn; jiangxiao4367@126.com; yhwang@scsio.ac.cn; hucq@scsio.ac.cn
2014
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume38Issue:1Pages:265-274
AbstractFerritin is one of the major non-harm iron storage proteins that found in most cell types of animals, plants and microorganisms. In this study, a ferritin subunit named StmFer was identified from the sea cucumber (Stichopus monotuberculatus) and characterized functionally. The full-length cDNA of StmFer is 1184 bp in size with a 5'-untranslated region (UTR) of 131 bp, a 3'-UTR of 531 bp and an open reading frame of 522 bp that encoding a protein of 173 amino acids with a deduced molecular weight of 19.95 kDa. StmFer possesses both the ferroxidase center of vertebrate ferritin heavy subunit and iron nucleation sites of vertebrate ferritin light subunit. For the gene structure, StmFer contains only three exons separated by two introns. Higher levels of mRNA expression were noticed in intestine and coelomocyte of S. monotuberculans by northern blot analysis. In in vitro experiments performed in coelomocytes, transcriptional expression of StmFer showed the strongest response to polyriboinosinic polyribocytidylic acid [Poly (I:C)] (9.08 fold up-regulation), followed by lipopolysaccharides (LPS), ferrous chloride (FeCl2) and inactivated bacteria (Vibrio alginolyticus) (7.84, 7.41 and 4.90 fold up-regulation, respectively) after 3 h post-challenge. In addition, the anti-oxidation activity and iron binding capacity of recombinant ferritin protein were demonstrated in this study. As a whole, our study suggested that the ferritin from sea cucumber may play critical roles not only in the cellular and organismic iron homeostasis, but also in the innate immune defense. (C) 2014 Elsevier Ltd. All rights reserved.
Department[Hu, Chaoqun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 510301, Guangdong, Peoples R China ; Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou 510301, Guangdong, Peoples R China ; Chinese Acad Sci, Chinese Acad Sci LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
KeywordFerritin Stichopus Monotuberculatus Anti-oxidation Activity Iron Binding Capacity Innate Immune Defense
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationGuangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272]
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Indexed Bysci
Funding ProjectLMB
Funding OrganizationGuangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272] ; Guangdong Province; Key Projects in the National Science & Technology Pillar Program [2012BAD18B03]; CAS Cooperation Program [2012B091100272]
WOS IDWOS:000336353700033
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10446
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorrosemary166@sina.com; chan1010@scsio.ac.cn; jiangxiao4367@126.com; yhwang@scsio.ac.cn; hucq@scsio.ac.cn
Recommended Citation
GB/T 7714
Ren, CH,Chen, T,Jiang, X,et al. Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2014,38(1):265-274.
APA Ren, CH.,Chen, T.,Jiang, X.,Wang, YH.,Hu, CQ.,...&hucq@scsio.ac.cn.(2014).Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus.FISH & SHELLFISH IMMUNOLOGY,38(1),265-274.
MLA Ren, CH,et al."Identification and functional characterization of a novel ferritin subunit from the tropical sea cucumber, Stichopus monotuberculatus".FISH & SHELLFISH IMMUNOLOGY 38.1(2014):265-274.
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