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学科主题: Pharmacology & Pharmacy
题名: Optimization of Medium Using Response Surface Methodology for Lipid Production by Scenedesmus sp.
作者: Yang, FF ; Long, LJ ; Sun, XM ; Wu, HL ; Li, T ; Xiang, WZ
通讯作者: ycuyang@163.com ; longlj@scsio.ac.cn ; sunxm@scsio.ac.cn ; hlwu@scsio.ac.cn ; taoli@scsio.ac.cn ; xwz@scsio.ac.cn
关键词: biodiesel ; lipid production ; response surface methodology ; Scenedesmus sp. ; optimization
刊名: MARINE DRUGS
发表日期: 2014
卷: 12, 期:3, 页:1245-1257
收录类别: sci
部门归属: [Yang, Fangfang ; Long, Lijuan ; Sun, Xiumei ; Wu, Hualian ; Li, Tao ; Xiang, Wenzhou] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Yang, Fangfang ; Sun, Xiumei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
项目归属: LMB
资助者: Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]; Guangdong Ocean Innovative Demonstration Area of Economic Development Project [GD2012-D01-002]
摘要: Lipid production is an important indicator for assessing microalgal species for biodiesel production. In this work, the effects of medium composition on lipid production by Scenedesmus sp. were investigated using the response surface methodology. The results of a Plackett-Burman design experiment revealed that NaHCO3, NaH2PO4 center dot 2H(2)O and NaNO3 were three factors significantly influencing lipid production, which were further optimized by a Box-Behnken design. The optimal medium was found to contain 3.07 g L-1 NaHCO3, 15.49 mg L-1 NaH2PO4 center dot 2H(2)O and 803.21 mg L-1 NaNO3. Using the optimal conditions previously determined, the lipid production (304.02 mg center dot L-1) increased 54.64% more than that using the initial medium, which agreed well with the predicted value 309.50 mg L-1. Additionally, lipid analysis found that palmitic acid (C16:0) and oleic acid (C18:1) dominantly constituted the algal fatty acids (about 60% of the total fatty acids) and a much higher content of neutral lipid accounted for 82.32% of total lipids, which strongly proved that Scenedesmus sp. is a very promising feedstock for biodiesel production.
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WOS记录号: WOS:000335752800005
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10462
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Yang, FF; Long, LJ; Sun, XM; Wu, HL; Li, T; Xiang, WZ.Optimization of Medium Using Response Surface Methodology for Lipid Production by Scenedesmus sp.,MARINE DRUGS,2014,12(3):1245-1257
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