Optimization of Medium Using Response Surface Methodology for Lipid Production by Scenedesmus sp.
Yang, FF; Long, LJ; Sun, XM; Wu, HL; Li, T; Xiang, WZ; ycuyang@163.com; longlj@scsio.ac.cn; sunxm@scsio.ac.cn; hlwu@scsio.ac.cn; taoli@scsio.ac.cn; xwz@scsio.ac.cn
2014
发表期刊MARINE DRUGS
ISSN1660-3397
卷号12期号:3页码:1245-1257
摘要Lipid production is an important indicator for assessing microalgal species for biodiesel production. In this work, the effects of medium composition on lipid production by Scenedesmus sp. were investigated using the response surface methodology. The results of a Plackett-Burman design experiment revealed that NaHCO3, NaH2PO4 center dot 2H(2)O and NaNO3 were three factors significantly influencing lipid production, which were further optimized by a Box-Behnken design. The optimal medium was found to contain 3.07 g L-1 NaHCO3, 15.49 mg L-1 NaH2PO4 center dot 2H(2)O and 803.21 mg L-1 NaNO3. Using the optimal conditions previously determined, the lipid production (304.02 mg center dot L-1) increased 54.64% more than that using the initial medium, which agreed well with the predicted value 309.50 mg L-1. Additionally, lipid analysis found that palmitic acid (C16:0) and oleic acid (C18:1) dominantly constituted the algal fatty acids (about 60% of the total fatty acids) and a much higher content of neutral lipid accounted for 82.32% of total lipids, which strongly proved that Scenedesmus sp. is a very promising feedstock for biodiesel production.
部门归属[Yang, Fangfang ; Long, Lijuan ; Sun, Xiumei ; Wu, Hualian ; Li, Tao ; Xiang, Wenzhou] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Yang, Fangfang ; Sun, Xiumei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
关键词Biodiesel Lipid Production Response Surface Methodology Scenedesmus Sp. Optimization
学科领域Pharmacology & Pharmacy
资助者Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]; Guangdong Ocean Innovative Demonstration Area of Economic Development Project [GD2012-D01-002] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]; Guangdong Ocean Innovative Demonstration Area of Economic Development Project [GD2012-D01-002] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]; Guangdong Ocean Innovative Demonstration Area of Economic Development Project [GD2012-D01-002] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]; Guangdong Ocean Innovative Demonstration Area of Economic Development Project [GD2012-D01-002]
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收录类别sci
资助项目LMB
资助者Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]; Guangdong Ocean Innovative Demonstration Area of Economic Development Project [GD2012-D01-002] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]; Guangdong Ocean Innovative Demonstration Area of Economic Development Project [GD2012-D01-002] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]; Guangdong Ocean Innovative Demonstration Area of Economic Development Project [GD2012-D01-002] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]; Guangdong Ocean Innovative Demonstration Area of Economic Development Project [GD2012-D01-002]
WOS记录号WOS:000335752800005
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被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10462
专题中科院海洋生物资源可持续利用重点实验室
通讯作者ycuyang@163.com; longlj@scsio.ac.cn; sunxm@scsio.ac.cn; hlwu@scsio.ac.cn; taoli@scsio.ac.cn; xwz@scsio.ac.cn
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Yang, FF,Long, LJ,Sun, XM,et al. Optimization of Medium Using Response Surface Methodology for Lipid Production by Scenedesmus sp.[J]. MARINE DRUGS,2014,12(3):1245-1257.
APA Yang, FF.,Long, LJ.,Sun, XM.,Wu, HL.,Li, T.,...&xwz@scsio.ac.cn.(2014).Optimization of Medium Using Response Surface Methodology for Lipid Production by Scenedesmus sp..MARINE DRUGS,12(3),1245-1257.
MLA Yang, FF,et al."Optimization of Medium Using Response Surface Methodology for Lipid Production by Scenedesmus sp.".MARINE DRUGS 12.3(2014):1245-1257.
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