A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature
Yang, FF; Xiang, WZ; Sun, XM; Wu, HL; Li, T; Long, LJ; ycuyang@163.com; xwz@scsio.ac.cn; sunxm@scsio.ac.cn; hlwu@scsio.ac.cn; taoli@scsio.ac.cn; longlj@scsio.ac.cn
2014
发表期刊MARINE DRUGS
ISSN1660-3397
卷号12期号:3页码:1258-1270
摘要A novel method using ethanol was proposed for extracting lipids from wet microalga Picochlorum sp. at room temperature and pressure. In this study, Central Composite design (CCD) was applied to investigate the optimum conditions of lipid extraction. The results revealed that the solvent to biomass ratio had the largest effect on lipid extraction efficiency, followed by extraction time and temperature. A high lipid extraction yield (33.04% of the dry weight) was obtained under the following extraction conditions: 5 mL solvents per gram of wet biomass for 37 min with gentle stirring at room temperature. The extraction yield was comparable to that obtained by the widely used Bligh-Dyer method. Furthermore, no significant differences in the distribution of lipid classes and fatty acid composition were observed according to different extraction methods. In conclusion, these results indicated that the proposed procedure using ethanol could extract lipids from wet biomass efficiently and had giant potential for lipid extraction at large scale.
部门归属[Yang, Fangfang ; Xiang, Wenzhou ; Sun, Xiumei ; Wu, Hualian ; Li, Tao ; Long, Lijuan] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Yang, Fangfang ; Sun, Xiumei] Chinese Acedemy Sci, Grad Sch, Beijing 100049, Peoples R China
关键词Lipid Extraction Wet Microalga Picochlorum Sp. Ethanol Biofuel
学科领域Pharmacology & Pharmacy
资助者Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]
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收录类别sci
资助项目LMB
资助者Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093] ; Ocean Public Welfare Scientific Research Project [201305018-3]; Funds for Marine Renewable Energy [GHME2011SW04]; Chinese Academy of Sciences [KSCX2-YW-R-093]
WOS记录号WOS:000335752800006
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被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10463
专题中科院海洋生物资源可持续利用重点实验室
通讯作者ycuyang@163.com; xwz@scsio.ac.cn; sunxm@scsio.ac.cn; hlwu@scsio.ac.cn; taoli@scsio.ac.cn; longlj@scsio.ac.cn
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Yang, FF,Xiang, WZ,Sun, XM,et al. A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature[J]. MARINE DRUGS,2014,12(3):1258-1270.
APA Yang, FF.,Xiang, WZ.,Sun, XM.,Wu, HL.,Li, T.,...&longlj@scsio.ac.cn.(2014).A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature.MARINE DRUGS,12(3),1258-1270.
MLA Yang, FF,et al."A Novel Lipid Extraction Method from Wet Microalga Picochlorum sp at Room Temperature".MARINE DRUGS 12.3(2014):1258-1270.
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