Pacific White Shrimp (Litopenaeus vannamei) Vitellogenesis-Inhibiting Hormone (VIH) Is Predominantly Expressed in the Brain and Negatively Regulates Hepatopancreatic Vitellogenin (VTG) Gene Expression
Chen, T; Zhang, LP; Wong, NK; Zhong, M; Ren, CH; Hu, CQ; rosemary166@sina.com
2014
发表期刊BIOLOGY OF REPRODUCTION
ISSN0006-3363
卷号90期号:3页码:-47
摘要Ovarian maturation in crustaceans is temporally orchestrated by two processes: oogenesis and vitellogenesis. The peptide hormone vitellogenesis-inhibiting hormone (VIH), by far the most potent negative regulator of crustacean reproduction known, critically modulates crustacean ovarian maturation by suppressing vitellogenin (VTG) synthesis. In this study, cDNA encoding VIH was cloned from the eyestalk of Pacific white shrimp, Litopenaeus vannamei, a highly significant commercial culture species. Phylogenetic analysis suggests that L. vannamei VIH (lvVIH) can be classified as a member of the type II crustacean hyperglycemic hormone family. Northern blot and RT-PCR results reveal that both the brain and eyestalk were the major sources for lvVIH mRNA expression. In in vitro experiments on primary culture of shrimp hepatopancreatic cells, it was confirmed that some endogenous inhibitory factors existed in L. vannamei hemolymph, brain, and eyestalk that suppressed hepatopancreatic VTG gene expression. Purified recombinant lvVIH protein was effective in inhibiting VTG mRNA expression in both in vitro primary hepatopancreatic cell culture and in vivo injection experiments. Injection of recombinant VIH could also reverse ovarian growth induced by eyestalk ablation. Furthermore, unilateral eyestalk ablation reduced the mRNA level of lvVIH in the brain but not in the remaining contralateral eyestalk. Our study, as a whole, provides new insights on VIH regulation of shrimp reproduction: 1) the brain and eyestalk are both important sites of VIH expression and therefore possible coregulators of hepatopancreatic VTG mRNA expression and 2) eyestalk ablation could increase hepatopancreatic VTG expression by transcriptionally abolishing eyestalk-derived VIH and diminishing brain-derived VIH.
部门归属[Chen, Ting ; Zhang, Lv-Ping ; Zhong, Ming ; Ren, Chun-Hua ; Hu, Chao-Qun] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Wong, Nai-Kei] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China ; [Zhong, Ming] Univ Chinese Acad Sci, Beijing, Peoples R China
关键词Brain Eyestalk Ablation Hepatopancreas Reproduction Shrimp Vih Vtg
学科领域Reproductive Biology
资助者National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4]
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收录类别sci
资助项目LMB
资助者National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4]
WOS记录号WOS:000332622700001
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被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10466
专题中科院海洋生物资源可持续利用重点实验室
通讯作者rosemary166@sina.com
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Chen, T,Zhang, LP,Wong, NK,et al. Pacific White Shrimp (Litopenaeus vannamei) Vitellogenesis-Inhibiting Hormone (VIH) Is Predominantly Expressed in the Brain and Negatively Regulates Hepatopancreatic Vitellogenin (VTG) Gene Expression[J]. BIOLOGY OF REPRODUCTION,2014,90(3):-47.
APA Chen, T.,Zhang, LP.,Wong, NK.,Zhong, M.,Ren, CH.,...&rosemary166@sina.com.(2014).Pacific White Shrimp (Litopenaeus vannamei) Vitellogenesis-Inhibiting Hormone (VIH) Is Predominantly Expressed in the Brain and Negatively Regulates Hepatopancreatic Vitellogenin (VTG) Gene Expression.BIOLOGY OF REPRODUCTION,90(3),-47.
MLA Chen, T,et al."Pacific White Shrimp (Litopenaeus vannamei) Vitellogenesis-Inhibiting Hormone (VIH) Is Predominantly Expressed in the Brain and Negatively Regulates Hepatopancreatic Vitellogenin (VTG) Gene Expression".BIOLOGY OF REPRODUCTION 90.3(2014):-47.
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