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学科主题: Reproductive Biology
题名: Pacific White Shrimp (Litopenaeus vannamei) Vitellogenesis-Inhibiting Hormone (VIH) Is Predominantly Expressed in the Brain and Negatively Regulates Hepatopancreatic Vitellogenin (VTG) Gene Expression
作者: Chen, T ; Zhang, LP ; Wong, NK ; Zhong, M ; Ren, CH ; Hu, CQ
通讯作者: rosemary166@sina.com
关键词: brain ; eyestalk ablation ; hepatopancreas ; reproduction ; shrimp ; VIH ; VTG
刊名: BIOLOGY OF REPRODUCTION
发表日期: 2014
卷: 90, 期:3, 页:-47
收录类别: sci
部门归属: [Chen, Ting ; Zhang, Lv-Ping ; Zhong, Ming ; Ren, Chun-Hua ; Hu, Chao-Qun] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Wong, Nai-Kei] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China ; [Zhong, Ming] Univ Chinese Acad Sci, Beijing, Peoples R China
项目归属: LMB
资助者: National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4]
摘要: Ovarian maturation in crustaceans is temporally orchestrated by two processes: oogenesis and vitellogenesis. The peptide hormone vitellogenesis-inhibiting hormone (VIH), by far the most potent negative regulator of crustacean reproduction known, critically modulates crustacean ovarian maturation by suppressing vitellogenin (VTG) synthesis. In this study, cDNA encoding VIH was cloned from the eyestalk of Pacific white shrimp, Litopenaeus vannamei, a highly significant commercial culture species. Phylogenetic analysis suggests that L. vannamei VIH (lvVIH) can be classified as a member of the type II crustacean hyperglycemic hormone family. Northern blot and RT-PCR results reveal that both the brain and eyestalk were the major sources for lvVIH mRNA expression. In in vitro experiments on primary culture of shrimp hepatopancreatic cells, it was confirmed that some endogenous inhibitory factors existed in L. vannamei hemolymph, brain, and eyestalk that suppressed hepatopancreatic VTG gene expression. Purified recombinant lvVIH protein was effective in inhibiting VTG mRNA expression in both in vitro primary hepatopancreatic cell culture and in vivo injection experiments. Injection of recombinant VIH could also reverse ovarian growth induced by eyestalk ablation. Furthermore, unilateral eyestalk ablation reduced the mRNA level of lvVIH in the brain but not in the remaining contralateral eyestalk. Our study, as a whole, provides new insights on VIH regulation of shrimp reproduction: 1) the brain and eyestalk are both important sites of VIH expression and therefore possible coregulators of hepatopancreatic VTG mRNA expression and 2) eyestalk ablation could increase hepatopancreatic VTG expression by transcriptionally abolishing eyestalk-derived VIH and diminishing brain-derived VIH.
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WOS记录号: WOS:000332622700001
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10466
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Chen, T; Zhang, LP; Wong, NK; Zhong, M; Ren, CH; Hu, CQ.Pacific White Shrimp (Litopenaeus vannamei) Vitellogenesis-Inhibiting Hormone (VIH) Is Predominantly Expressed in the Brain and Negatively Regulates Hepatopancreatic Vitellogenin (VTG) Gene Expression,BIOLOGY OF REPRODUCTION,2014,90(3):-47
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