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Pacific White Shrimp (Litopenaeus vannamei) Vitellogenesis-Inhibiting Hormone (VIH) Is Predominantly Expressed in the Brain and Negatively Regulates Hepatopancreatic Vitellogenin (VTG) Gene Expression
Chen, T; Zhang, LP; Wong, NK; Zhong, M; Ren, CH; Hu, CQ; rosemary166@sina.com
2014
Source PublicationBIOLOGY OF REPRODUCTION
ISSN0006-3363
Volume90Issue:3Pages:-47
AbstractOvarian maturation in crustaceans is temporally orchestrated by two processes: oogenesis and vitellogenesis. The peptide hormone vitellogenesis-inhibiting hormone (VIH), by far the most potent negative regulator of crustacean reproduction known, critically modulates crustacean ovarian maturation by suppressing vitellogenin (VTG) synthesis. In this study, cDNA encoding VIH was cloned from the eyestalk of Pacific white shrimp, Litopenaeus vannamei, a highly significant commercial culture species. Phylogenetic analysis suggests that L. vannamei VIH (lvVIH) can be classified as a member of the type II crustacean hyperglycemic hormone family. Northern blot and RT-PCR results reveal that both the brain and eyestalk were the major sources for lvVIH mRNA expression. In in vitro experiments on primary culture of shrimp hepatopancreatic cells, it was confirmed that some endogenous inhibitory factors existed in L. vannamei hemolymph, brain, and eyestalk that suppressed hepatopancreatic VTG gene expression. Purified recombinant lvVIH protein was effective in inhibiting VTG mRNA expression in both in vitro primary hepatopancreatic cell culture and in vivo injection experiments. Injection of recombinant VIH could also reverse ovarian growth induced by eyestalk ablation. Furthermore, unilateral eyestalk ablation reduced the mRNA level of lvVIH in the brain but not in the remaining contralateral eyestalk. Our study, as a whole, provides new insights on VIH regulation of shrimp reproduction: 1) the brain and eyestalk are both important sites of VIH expression and therefore possible coregulators of hepatopancreatic VTG mRNA expression and 2) eyestalk ablation could increase hepatopancreatic VTG expression by transcriptionally abolishing eyestalk-derived VIH and diminishing brain-derived VIH.
Department[Chen, Ting ; Zhang, Lv-Ping ; Zhong, Ming ; Ren, Chun-Hua ; Hu, Chao-Qun] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Wong, Nai-Kei] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China ; [Zhong, Ming] Univ Chinese Acad Sci, Beijing, Peoples R China
KeywordBrain Eyestalk Ablation Hepatopancreas Reproduction Shrimp Vih Vtg
Subject AreaReproductive Biology
Funding OrganizationNational High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4]
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Indexed Bysci
Funding ProjectLMB
Funding OrganizationNational High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4] ; National High Technology Research & Development Program of China (863 Program) [2012AA10A404-4]
WOS IDWOS:000332622700001
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10466
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorrosemary166@sina.com
Recommended Citation
GB/T 7714
Chen, T,Zhang, LP,Wong, NK,et al. Pacific White Shrimp (Litopenaeus vannamei) Vitellogenesis-Inhibiting Hormone (VIH) Is Predominantly Expressed in the Brain and Negatively Regulates Hepatopancreatic Vitellogenin (VTG) Gene Expression[J]. BIOLOGY OF REPRODUCTION,2014,90(3):-47.
APA Chen, T.,Zhang, LP.,Wong, NK.,Zhong, M.,Ren, CH.,...&rosemary166@sina.com.(2014).Pacific White Shrimp (Litopenaeus vannamei) Vitellogenesis-Inhibiting Hormone (VIH) Is Predominantly Expressed in the Brain and Negatively Regulates Hepatopancreatic Vitellogenin (VTG) Gene Expression.BIOLOGY OF REPRODUCTION,90(3),-47.
MLA Chen, T,et al."Pacific White Shrimp (Litopenaeus vannamei) Vitellogenesis-Inhibiting Hormone (VIH) Is Predominantly Expressed in the Brain and Negatively Regulates Hepatopancreatic Vitellogenin (VTG) Gene Expression".BIOLOGY OF REPRODUCTION 90.3(2014):-47.
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