IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis
Fu, JP; Chen, SN; Zou, PF; Huang, B; Guo, Z; Zeng, LB; Qin, QW; Nie, P; pinnie@ihb.ac.cn
2014
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
卷号43期号:1页码:87-95
摘要The IFN-gamma gene was identified in a turtle, the Chinese soft-shelled turtle, Pelodiscus sinensis, with its genome consisting of 4 exons and 3 introns. The deduced amino acid sequence of this gene contains a signal peptide, an IFN-gamma family signature motif (130)IQRKAVNELFPT, an NLS motif (KRKR)-K-155 and three potential N-glycosylation sites. As revealed by real-time quantitative PCR, the gene was constitutively expressed in all tested organs/tissues, with higher level observed in blood, intestine and thymus. An induced expression of IFN-gamma at mRNA level was observed in peripheral blood leucocytes (PBLs) in response to in vitro stimulation of LPS and PolyI:C. The overexpression of IFN-gamma in the Chinese soft-shelled turtle artery (STA) cell line resulted in the increase in the expression of transcriptional regulators, such as IRF1, IRF7 and STAT1, and antiviral genes, such as Mx, PKR, implying possibly the existence of a conserved signalling network and role for IFN-gamma in the turtle. Furthermore, the infection of soft-shelled turtle iridovirus (STIV) in the cell line transfected with IFN-gamma may cause the cell death as demonstrated with the elevated lactate dehydrogenase (LDH) level and cell mortality. However, the mechanism involved in the antiviral activity may require further investigation. (C) 2013 Elsevier Ltd. All rights reserved.
部门归属[Fu, Jian Ping ; Chen, Shan Nan ; Zou, Peng Fei ; Guo, Zheng ; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Fu, Jian Ping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Huang, Bei ; Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China ; [Zeng, Ling Bing] Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei Province, Peoples R China ; [Qin, Qi Wei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
关键词Ifn-gamma Ifn-gamma Stimulated Gene Iridovirus Antiviral Activity Turtle
学科领域Immunology ; Zoology
资助者National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703]
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收录类别sci
资助项目LMB
资助者National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703] ; National Natural Science Foundation of China [30230075]; National Basic Research Program (973 Program) of China [2009CB118703]
WOS记录号WOS:000331479800012
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10468
专题中科院海洋生物资源可持续利用重点实验室
通讯作者pinnie@ihb.ac.cn
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Fu, JP,Chen, SN,Zou, PF,et al. IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,43(1):87-95.
APA Fu, JP.,Chen, SN.,Zou, PF.,Huang, B.,Guo, Z.,...&pinnie@ihb.ac.cn.(2014).IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,43(1),87-95.
MLA Fu, JP,et al."IFN-gamma in turtle: Conservation in sequence and signalling and role in inhibiting iridovirus replication in Chinese soft-shelled turtle Pelodiscus sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 43.1(2014):87-95.
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