Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides
Ji, H; Wei, J; Wei, S; Yan, Y; Huang, Y; Huang, X; Zhou, S; Zhou, Y; Qin, Q; qinqw@scsio.ac.cn
2014
发表期刊JOURNAL OF FISH BIOLOGY
ISSN0022-1112
卷号84期号:2页码:436-447
摘要A C-type lectin-like protein (Ec-CTLP) was cloned from the grouper Epinephelus coioides. The full-length cDNA of Ec-CTLP was composed of 905 bp with a 522 bp open reading frame that encodes a 174-residue protein. The putative amino acid sequence of Ec-CTLP contains a signal peptide of 19 residues at the N-terminus and a CLECT domain from Cys43 to Arg169 and a conserved imperfect WND (Trp-Asn-Asp) motif. The homologous identity of deduced amino acid sequences is from 32 to 42% with other fishes. The expression of Ec-CTLP was differently upregulated in E. coioides spleen (germline stem) cells after being challenged at 16 and 4 degrees C. Intracellular localization revealed that Ec-CTLP was distributed only in the cytoplasm. Recombinant Ec-CTLP (rEc-CTLP) was expressed in Escherichia coli BL21 (DE3) and purified for mouse Mus musculus anti-Ec-CTLP serum preparation. The rEc-CTLP fusion protein does not possess haemagglutinating activity, but improves survival from frozen bacteria. The survival of bacteria (including gram-negative E. coli and gram-positive Staphylococcus aureus) was positively correlated with the concentration of the rEc-CTLP. These findings can provide clues to help understand the probable C-type lectin in marine fish innate immunity. (C) 2014 The Fisheries Society of the British Isles
部门归属[Ji, H. ; Zhou, Y.] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China ; [Wei, J. ; Wei, S. ; Yan, Y. ; Huang, Y. ; Huang, X. ; Zhou, S. ; Qin, Q.] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
关键词Bacteria Freezing-survival Test Grouper Spleen Cells Haemagglutinating Activity Intracellular Localization
学科领域Fisheries ; Marine & Freshwater Biology
资助者National Basic Research Program of China (973 programme) [2012CB114402]; National High Technology Development Program of China (863) [2012AA092201]; national sparking plan project [2013GA780001]; "Strategic Priority Research Program" of Chinese Academy of Sciences [XDA11020203, XDA11030404]; Knowledge Innovation Program of the Chinese Academy of Sciences; National Natural Science Foundation of China [30930070, 31001124, 30725027, 31202022]; Guangdong Province Natural Science Foundation [2012A020603018] ; National Basic Research Program of China (973 programme) [2012CB114402]; National High Technology Development Program of China (863) [2012AA092201]; national sparking plan project [2013GA780001]; "Strategic Priority Research Program" of Chinese Academy of Sciences [XDA11020203, XDA11030404]; Knowledge Innovation Program of the Chinese Academy of Sciences; National Natural Science Foundation of China [30930070, 31001124, 30725027, 31202022]; Guangdong Province Natural Science Foundation [2012A020603018] ; National Basic Research Program of China (973 programme) [2012CB114402]; National High Technology Development Program of China (863) [2012AA092201]; national sparking plan project [2013GA780001]; "Strategic Priority Research Program" of Chinese Academy of Sciences [XDA11020203, XDA11030404]; Knowledge Innovation Program of the Chinese Academy of Sciences; National Natural Science Foundation of China [30930070, 31001124, 30725027, 31202022]; Guangdong Province Natural Science Foundation [2012A020603018] ; National Basic Research Program of China (973 programme) [2012CB114402]; National High Technology Development Program of China (863) [2012AA092201]; national sparking plan project [2013GA780001]; "Strategic Priority Research Program" of Chinese Academy of Sciences [XDA11020203, XDA11030404]; Knowledge Innovation Program of the Chinese Academy of Sciences; National Natural Science Foundation of China [30930070, 31001124, 30725027, 31202022]; Guangdong Province Natural Science Foundation [2012A020603018]
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收录类别sci
资助项目LMB
资助者National Basic Research Program of China (973 programme) [2012CB114402]; National High Technology Development Program of China (863) [2012AA092201]; national sparking plan project [2013GA780001]; "Strategic Priority Research Program" of Chinese Academy of Sciences [XDA11020203, XDA11030404]; Knowledge Innovation Program of the Chinese Academy of Sciences; National Natural Science Foundation of China [30930070, 31001124, 30725027, 31202022]; Guangdong Province Natural Science Foundation [2012A020603018] ; National Basic Research Program of China (973 programme) [2012CB114402]; National High Technology Development Program of China (863) [2012AA092201]; national sparking plan project [2013GA780001]; "Strategic Priority Research Program" of Chinese Academy of Sciences [XDA11020203, XDA11030404]; Knowledge Innovation Program of the Chinese Academy of Sciences; National Natural Science Foundation of China [30930070, 31001124, 30725027, 31202022]; Guangdong Province Natural Science Foundation [2012A020603018] ; National Basic Research Program of China (973 programme) [2012CB114402]; National High Technology Development Program of China (863) [2012AA092201]; national sparking plan project [2013GA780001]; "Strategic Priority Research Program" of Chinese Academy of Sciences [XDA11020203, XDA11030404]; Knowledge Innovation Program of the Chinese Academy of Sciences; National Natural Science Foundation of China [30930070, 31001124, 30725027, 31202022]; Guangdong Province Natural Science Foundation [2012A020603018] ; National Basic Research Program of China (973 programme) [2012CB114402]; National High Technology Development Program of China (863) [2012AA092201]; national sparking plan project [2013GA780001]; "Strategic Priority Research Program" of Chinese Academy of Sciences [XDA11020203, XDA11030404]; Knowledge Innovation Program of the Chinese Academy of Sciences; National Natural Science Foundation of China [30930070, 31001124, 30725027, 31202022]; Guangdong Province Natural Science Foundation [2012A020603018]
WOS记录号WOS:000336083200011
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10471
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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Ji, H,Wei, J,Wei, S,et al. Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides[J]. JOURNAL OF FISH BIOLOGY,2014,84(2):436-447.
APA Ji, H.,Wei, J.,Wei, S.,Yan, Y.,Huang, Y.,...&qinqw@scsio.ac.cn.(2014).Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides.JOURNAL OF FISH BIOLOGY,84(2),436-447.
MLA Ji, H,et al."Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides".JOURNAL OF FISH BIOLOGY 84.2(2014):436-447.
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