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学科主题: Fisheries ; Marine & Freshwater Biology
题名: Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides
作者: Ji, H ; Wei, J ; Wei, S ; Yan, Y ; Huang, Y ; Huang, X ; Zhou, S ; Zhou, Y ; Qin, Q
通讯作者: qinqw@scsio.ac.cn
关键词: bacteria freezing-survival test ; grouper spleen cells ; haemagglutinating activity ; intracellular localization
刊名: JOURNAL OF FISH BIOLOGY
发表日期: 2014
卷: 84, 期:2, 页:436-447
收录类别: sci
部门归属: [Ji, H. ; Zhou, Y.] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China ; [Wei, J. ; Wei, S. ; Yan, Y. ; Huang, Y. ; Huang, X. ; Zhou, S. ; Qin, Q.] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China (973 programme) [2012CB114402]; National High Technology Development Program of China (863) [2012AA092201]; national sparking plan project [2013GA780001]; "Strategic Priority Research Program" of Chinese Academy of Sciences [XDA11020203, XDA11030404]; Knowledge Innovation Program of the Chinese Academy of Sciences; National Natural Science Foundation of China [30930070, 31001124, 30725027, 31202022]; Guangdong Province Natural Science Foundation [2012A020603018]
摘要: A C-type lectin-like protein (Ec-CTLP) was cloned from the grouper Epinephelus coioides. The full-length cDNA of Ec-CTLP was composed of 905 bp with a 522 bp open reading frame that encodes a 174-residue protein. The putative amino acid sequence of Ec-CTLP contains a signal peptide of 19 residues at the N-terminus and a CLECT domain from Cys43 to Arg169 and a conserved imperfect WND (Trp-Asn-Asp) motif. The homologous identity of deduced amino acid sequences is from 32 to 42% with other fishes. The expression of Ec-CTLP was differently upregulated in E. coioides spleen (germline stem) cells after being challenged at 16 and 4 degrees C. Intracellular localization revealed that Ec-CTLP was distributed only in the cytoplasm. Recombinant Ec-CTLP (rEc-CTLP) was expressed in Escherichia coli BL21 (DE3) and purified for mouse Mus musculus anti-Ec-CTLP serum preparation. The rEc-CTLP fusion protein does not possess haemagglutinating activity, but improves survival from frozen bacteria. The survival of bacteria (including gram-negative E. coli and gram-positive Staphylococcus aureus) was positively correlated with the concentration of the rEc-CTLP. These findings can provide clues to help understand the probable C-type lectin in marine fish innate immunity. (C) 2014 The Fisheries Society of the British Isles
原文出处: 查看原文
WOS记录号: WOS:000336083200011
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10471
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Ji, H; Wei, J; Wei, S; Yan, Y; Huang, Y; Huang, X; Zhou, S; Zhou, Y; Qin, Q.Molecular cloning and expression of a C-type lectin-like protein from orange-spotted grouper Epinephelus coioides,JOURNAL OF FISH BIOLOGY,2014,84(2):436-447
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