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学科主题: Biochemistry & Molecular Biology
题名: RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
作者: Guo, YX ; Quiroga, C ; Chen, Q ; McAnulty, MJ ; Benedik, MJ ; Wood, TK ; Wang, XX
通讯作者: twood@engr.psu.edu ; xxwang@scsio.ac.cn
刊名: NUCLEIC ACIDS RESEARCH
发表日期: 2014
卷: 42, 期:10, 页:6448-6462
收录类别: sci
部门归属: [Guo, Yunxue ; Wang, Xiaoxue] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Quiroga, Cecilia ; Chen, Qin ; McAnulty, Michael J. ; Wood, Thomas K.] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA ; [Benedik, Michael J.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA ; [Wood, Thomas K.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
项目归属: LMB
资助者: National Basic Research Program of China [2013CB955701]; National Natural Science Foundation of China [NFSC31270214, NFSC31290233]; National Institutes of Health [NIH R01 GM089999]; 1000-Youth Elite Program (the Recruitment Program of Global Experts in China)
摘要: For toxin/antitoxin (TA) systems, no toxin has been identified that functions by cleaving DNA. Here, we demonstrate that RalR and RalA of the cryptic prophage rac form a type I TA pair in which the antitoxin RNA is a trans-encoded small RNA with 16 nucleotides of complementarity to the toxin mRNA. We suggest the newly discovered antitoxin gene be named ralA for RalR antitoxin. Toxin RalR functions as a non-specific endonuclease that cleaves methylated and unmethylated DNA. The RNA chaperone Hfq is required for RalA antitoxin activity and appears to stabilize RalA. Also, RalR/RalA is beneficial to the Escherichia coli host for responding to the antibiotic fosfomycin. Hence, our results indicate that cryptic prophage genes can be functionally divergent from their active phage counterparts after integration into the host genome.
原文出处: 查看原文
WOS记录号: WOS:000338768100034
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10481
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
Guo, YX; Quiroga, C; Chen, Q; McAnulty, MJ; Benedik, MJ; Wood, TK; Wang, XX.RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli,NUCLEIC ACIDS RESEARCH,2014,42(10):6448-6462
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