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RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
Guo, YX; Quiroga, C; Chen, Q; McAnulty, MJ; Benedik, MJ; Wood, TK; Wang, XX; twood@engr.psu.edu; xxwang@scsio.ac.cn
2014
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
Volume42Issue:10Pages:6448-6462
AbstractFor toxin/antitoxin (TA) systems, no toxin has been identified that functions by cleaving DNA. Here, we demonstrate that RalR and RalA of the cryptic prophage rac form a type I TA pair in which the antitoxin RNA is a trans-encoded small RNA with 16 nucleotides of complementarity to the toxin mRNA. We suggest the newly discovered antitoxin gene be named ralA for RalR antitoxin. Toxin RalR functions as a non-specific endonuclease that cleaves methylated and unmethylated DNA. The RNA chaperone Hfq is required for RalA antitoxin activity and appears to stabilize RalA. Also, RalR/RalA is beneficial to the Escherichia coli host for responding to the antibiotic fosfomycin. Hence, our results indicate that cryptic prophage genes can be functionally divergent from their active phage counterparts after integration into the host genome.
Department[Guo, Yunxue ; Wang, Xiaoxue] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Quiroga, Cecilia ; Chen, Qin ; McAnulty, Michael J. ; Wood, Thomas K.] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA ; [Benedik, Michael J.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA ; [Wood, Thomas K.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
Subject AreaBiochemistry & Molecular Biology
Funding OrganizationNational Basic Research Program of China [2013CB955701]; National Natural Science Foundation of China [NFSC31270214, NFSC31290233]; National Institutes of Health [NIH R01 GM089999]; 1000-Youth Elite Program (the Recruitment Program of Global Experts in China) ; National Basic Research Program of China [2013CB955701]; National Natural Science Foundation of China [NFSC31270214, NFSC31290233]; National Institutes of Health [NIH R01 GM089999]; 1000-Youth Elite Program (the Recruitment Program of Global Experts in China) ; National Basic Research Program of China [2013CB955701]; National Natural Science Foundation of China [NFSC31270214, NFSC31290233]; National Institutes of Health [NIH R01 GM089999]; 1000-Youth Elite Program (the Recruitment Program of Global Experts in China) ; National Basic Research Program of China [2013CB955701]; National Natural Science Foundation of China [NFSC31270214, NFSC31290233]; National Institutes of Health [NIH R01 GM089999]; 1000-Youth Elite Program (the Recruitment Program of Global Experts in China)
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Indexed Bysci
Funding ProjectLMB
Funding OrganizationNational Basic Research Program of China [2013CB955701]; National Natural Science Foundation of China [NFSC31270214, NFSC31290233]; National Institutes of Health [NIH R01 GM089999]; 1000-Youth Elite Program (the Recruitment Program of Global Experts in China) ; National Basic Research Program of China [2013CB955701]; National Natural Science Foundation of China [NFSC31270214, NFSC31290233]; National Institutes of Health [NIH R01 GM089999]; 1000-Youth Elite Program (the Recruitment Program of Global Experts in China) ; National Basic Research Program of China [2013CB955701]; National Natural Science Foundation of China [NFSC31270214, NFSC31290233]; National Institutes of Health [NIH R01 GM089999]; 1000-Youth Elite Program (the Recruitment Program of Global Experts in China) ; National Basic Research Program of China [2013CB955701]; National Natural Science Foundation of China [NFSC31270214, NFSC31290233]; National Institutes of Health [NIH R01 GM089999]; 1000-Youth Elite Program (the Recruitment Program of Global Experts in China)
WOS IDWOS:000338768100034
Citation statistics
Cited Times:42[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10481
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authortwood@engr.psu.edu; xxwang@scsio.ac.cn
Recommended Citation
GB/T 7714
Guo, YX,Quiroga, C,Chen, Q,et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli[J]. NUCLEIC ACIDS RESEARCH,2014,42(10):6448-6462.
APA Guo, YX.,Quiroga, C.,Chen, Q.,McAnulty, MJ.,Benedik, MJ.,...&xxwang@scsio.ac.cn.(2014).RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli.NUCLEIC ACIDS RESEARCH,42(10),6448-6462.
MLA Guo, YX,et al."RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli".NUCLEIC ACIDS RESEARCH 42.10(2014):6448-6462.
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