ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis
Cai, BN; Pan, JY; Wan, P; Chen, DK; Long, SJ; Sun, HL; jypan@scsio.ac.cn
2014
发表期刊PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
ISSN1082-6068
卷号44期号:7页码:708-724
摘要The beneficial effects of oyster extract against various disorders and diseases induced by oxidative stress have aroused great interest. In this article, ultrasonic-assisted enzymolysis was employed to produce polysaccharides of Crassostrea hongkongensis (CHP) and their antioxidant activity was investigated. A single-factor experiment and then a four-factor, three-level Box-Behnken design were first used to optimize ultrasonic extraction for polysaccharides. On the basis of ridge analysis, the optimum conditions are obtained as ultrasonic treatment time of 24 min, power of 876 W, temperature of 49 degrees C, and material-solvent ratio of 1:6 (w/v). It is found that ultrasound pretreatment before protease hydrolysis was a great help to improve CHP yield and purity, especially more favorable with flavorzyme, neutrase, alcalase, and pepsin. Furthermore, the polysaccharide fraction, which was obtained by ultrasonic pretreatment and then alcalase hydrolysis at the conditions of 3000 U/g, 55 degrees C, pH 8.0, for 4 hr, exhibited an obvious scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical (98.48 +/- 0.55% and 99.20 +/- 0.12%, respectively) and a lenoleic acid peroxidation inhibition effect (85.48 +/- 0.65%) at a concentration of 5.0 mg/mL. These results reveal the potential application of CHP in functional food and nutraceuticals.
部门归属[Cai, Bingna ; Pan, Jianyu ; Wan, Peng ; Chen, Deke ; Long, Shujun ; Sun, Huili] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Wan, Peng ; Chen, Deke ; Long, Shujun ; Sun, Huili] Univ Chinese Acad Sci, Beijing, Peoples R China
关键词Antioxidant Crassostrea Hongkongensis Enzymolysis Polysaccharides Ultrasonic
学科领域Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
资助者Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017]
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收录类别sci
资助项目LMB
资助者Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017]
WOS记录号WOS:000338028600006
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10488
专题中科院海洋生物资源可持续利用重点实验室
通讯作者jypan@scsio.ac.cn
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Cai, BN,Pan, JY,Wan, P,et al. ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2014,44(7):708-724.
APA Cai, BN.,Pan, JY.,Wan, P.,Chen, DK.,Long, SJ.,...&jypan@scsio.ac.cn.(2014).ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,44(7),708-724.
MLA Cai, BN,et al."ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 44.7(2014):708-724.
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