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学科主题: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
题名: ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis
作者: Cai, BN ; Pan, JY ; Wan, P ; Chen, DK ; Long, SJ ; Sun, HL
通讯作者: jypan@scsio.ac.cn
关键词: antioxidant ; Crassostrea hongkongensis ; enzymolysis ; polysaccharides ; ultrasonic
刊名: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
发表日期: 2014
卷: 44, 期:7, 页:708-724
收录类别: sci
部门归属: [Cai, Bingna ; Pan, Jianyu ; Wan, Peng ; Chen, Deke ; Long, Shujun ; Sun, Huili] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Wan, Peng ; Chen, Deke ; Long, Shujun ; Sun, Huili] Univ Chinese Acad Sci, Beijing, Peoples R China
项目归属: LMB
资助者: Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017]
摘要: The beneficial effects of oyster extract against various disorders and diseases induced by oxidative stress have aroused great interest. In this article, ultrasonic-assisted enzymolysis was employed to produce polysaccharides of Crassostrea hongkongensis (CHP) and their antioxidant activity was investigated. A single-factor experiment and then a four-factor, three-level Box-Behnken design were first used to optimize ultrasonic extraction for polysaccharides. On the basis of ridge analysis, the optimum conditions are obtained as ultrasonic treatment time of 24 min, power of 876 W, temperature of 49 degrees C, and material-solvent ratio of 1:6 (w/v). It is found that ultrasound pretreatment before protease hydrolysis was a great help to improve CHP yield and purity, especially more favorable with flavorzyme, neutrase, alcalase, and pepsin. Furthermore, the polysaccharide fraction, which was obtained by ultrasonic pretreatment and then alcalase hydrolysis at the conditions of 3000 U/g, 55 degrees C, pH 8.0, for 4 hr, exhibited an obvious scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical (98.48 +/- 0.55% and 99.20 +/- 0.12%, respectively) and a lenoleic acid peroxidation inhibition effect (85.48 +/- 0.65%) at a concentration of 5.0 mg/mL. These results reveal the potential application of CHP in functional food and nutraceuticals.
原文出处: 查看原文
WOS记录号: WOS:000338028600006
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10488
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Cai, BN; Pan, JY; Wan, P; Chen, DK; Long, SJ; Sun, HL.ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis,PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2014,44(7):708-724
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