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ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis
Cai, BN; Pan, JY; Wan, P; Chen, DK; Long, SJ; Sun, HL; jypan@scsio.ac.cn
2014
Source PublicationPREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
ISSN1082-6068
Volume44Issue:7Pages:708-724
AbstractThe beneficial effects of oyster extract against various disorders and diseases induced by oxidative stress have aroused great interest. In this article, ultrasonic-assisted enzymolysis was employed to produce polysaccharides of Crassostrea hongkongensis (CHP) and their antioxidant activity was investigated. A single-factor experiment and then a four-factor, three-level Box-Behnken design were first used to optimize ultrasonic extraction for polysaccharides. On the basis of ridge analysis, the optimum conditions are obtained as ultrasonic treatment time of 24 min, power of 876 W, temperature of 49 degrees C, and material-solvent ratio of 1:6 (w/v). It is found that ultrasound pretreatment before protease hydrolysis was a great help to improve CHP yield and purity, especially more favorable with flavorzyme, neutrase, alcalase, and pepsin. Furthermore, the polysaccharide fraction, which was obtained by ultrasonic pretreatment and then alcalase hydrolysis at the conditions of 3000 U/g, 55 degrees C, pH 8.0, for 4 hr, exhibited an obvious scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical (98.48 +/- 0.55% and 99.20 +/- 0.12%, respectively) and a lenoleic acid peroxidation inhibition effect (85.48 +/- 0.65%) at a concentration of 5.0 mg/mL. These results reveal the potential application of CHP in functional food and nutraceuticals.
Department[Cai, Bingna ; Pan, Jianyu ; Wan, Peng ; Chen, Deke ; Long, Shujun ; Sun, Huili] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Wan, Peng ; Chen, Deke ; Long, Shujun ; Sun, Huili] Univ Chinese Acad Sci, Beijing, Peoples R China
KeywordAntioxidant Crassostrea Hongkongensis Enzymolysis Polysaccharides Ultrasonic
Subject AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
Funding OrganizationProject of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017]
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Funding ProjectLMB
Funding OrganizationProject of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017] ; Project of National Key Technology Research and Development Program for the 12th Five-year Plan [2012BAD33B10]; Public Science and Technology Research Funds Projects of Ocean [201305018-2]; Chinese Academy of Sciences [KZCX2-EW-Q214, 2011B090300057]; National Science Foundation for Young Scientists of China [31101271]; Guangdong Province [2011B090300057]; Frontier Science Program for Young Scientists of South China Sea Institute of Oceanology, Chinese Academy of Science [SQ201017]
WOS IDWOS:000338028600006
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10488
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorjypan@scsio.ac.cn
Recommended Citation
GB/T 7714
Cai, BN,Pan, JY,Wan, P,et al. ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2014,44(7):708-724.
APA Cai, BN.,Pan, JY.,Wan, P.,Chen, DK.,Long, SJ.,...&jypan@scsio.ac.cn.(2014).ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,44(7),708-724.
MLA Cai, BN,et al."ULTRASONIC-ASSISTED PRODUCTION OF ANTIOXIDATIVE POLYSACCHARIDES FROM Crassostrea hongkongensis".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 44.7(2014):708-724.
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