Identification and characterization of Rab7 from orange-spotted grouper, Epinephelus coioides
Fu, J; Huang, YH; Cai, J; Wei, SN; Ouyang, ZL; Ye, FZ; Huang, XH; Qin, QW; huangxh@scsio.ac.cn; qinqw@scsio.ac.cn
2014
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号36期号:1页码:19-26
摘要Rab7 is a small GTPase that regulates vesicular traffic from early to late endosomal stages of the endocytic pathway. During the virus-host co-evolution, host Rab7 was also exploited by virus to complete their life cycle. To date, however, the roles of fish Rab7 in virus infection remained largely unknown. Here, we cloned and characterized a Rab7 gene from grouper, Epinephelus coioides (Ec-Rab7). The full-length Ec-Rab7 cDNA was composed of 1182 bp and encoded a polypeptide of 207 amino acids which shared 99% identity with that from Anoplopoma fimbria or Oreochromis niloticus. Ec-Rab7 contained five conserved domains of Rab GTPase family including GTP-binding or GTPase regions as well as an effector site. RT-PCR analysis revealed that Ec-Rab7 ubiquitously expressed in all detected tissues and its transcript in spleen was up-regulated after challenge with Singapore grouper iridovirus (SGIV). Subcellular localization analysis revealed that Ec-Rab7 was distributed in the cytoplasm as spots and mostly colocalized with lysosomes. Notably, the ectopic expressed Ec-Rab7 partly aggregated into the viral factories in cells infected by SGIV. Furthermore, overexpression of Ec-Rab7 accelerated the occurrence of cytopathic effect (CPE) induced by SGIV infection and promoted viral gene transcription. In addition, far western blotting assay revealed that Ec-Rab7 might interact with viral proteins, including SGIV VP69 and VP101. Taken together, our data suggested that Ec-Rab7 might be potentially involved in SGIV replication. (C) 2013 Elsevier Ltd. All rights reserved.
部门归属[Fu, Jing ; Huang, Youhua ; Cai, Jia ; Wei, Shina ; Ouyang, Zhengliang ; Huang, Xiaohong ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Fu, Jing ; Cai, Jia ; Wei, Shina] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Ye, Fuzhou] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
关键词Epinephelus Coioides Rab7 Lysosome Singapore Grouper Iridovirus (Sgiv) Virus Replication
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070, 30800846, 30725027]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070, 30800846, 30725027]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070, 30800846, 30725027]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070, 30800846, 30725027]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213]
URL查看原文
收录类别sci
资助项目LMB
资助者National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070, 30800846, 30725027]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070, 30800846, 30725027]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070, 30800846, 30725027]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070, 30800846, 30725027]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213]
WOS记录号WOS:000331671600003
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10491
专题中科院海洋生物资源可持续利用重点实验室
通讯作者huangxh@scsio.ac.cn; qinqw@scsio.ac.cn
推荐引用方式
GB/T 7714
Fu, J,Huang, YH,Cai, J,et al. Identification and characterization of Rab7 from orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2014,36(1):19-26.
APA Fu, J.,Huang, YH.,Cai, J.,Wei, SN.,Ouyang, ZL.,...&qinqw@scsio.ac.cn.(2014).Identification and characterization of Rab7 from orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,36(1),19-26.
MLA Fu, J,et al."Identification and characterization of Rab7 from orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 36.1(2014):19-26.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Identification and c(2132KB) 开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。