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学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Identification and characterization of Rab7 from orange-spotted grouper, Epinephelus coioides
作者: Fu, J ; Huang, YH ; Cai, J ; Wei, SN ; Ouyang, ZL ; Ye, FZ ; Huang, XH ; Qin, QW
通讯作者: huangxh@scsio.ac.cn ; qinqw@scsio.ac.cn
关键词: Epinephelus coioides ; Rab7 ; Lysosome ; Singapore grouper iridovirus (SGIV) ; Virus replication
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2014
卷: 36, 期:1, 页:19-26
收录类别: sci
部门归属: [Fu, Jing ; Huang, Youhua ; Cai, Jia ; Wei, Shina ; Ouyang, Zhengliang ; Huang, Xiaohong ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Fu, Jing ; Cai, Jia ; Wei, Shina] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Ye, Fuzhou] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070, 30800846, 30725027]; Knowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-EW-Q213]
摘要: Rab7 is a small GTPase that regulates vesicular traffic from early to late endosomal stages of the endocytic pathway. During the virus-host co-evolution, host Rab7 was also exploited by virus to complete their life cycle. To date, however, the roles of fish Rab7 in virus infection remained largely unknown. Here, we cloned and characterized a Rab7 gene from grouper, Epinephelus coioides (Ec-Rab7). The full-length Ec-Rab7 cDNA was composed of 1182 bp and encoded a polypeptide of 207 amino acids which shared 99% identity with that from Anoplopoma fimbria or Oreochromis niloticus. Ec-Rab7 contained five conserved domains of Rab GTPase family including GTP-binding or GTPase regions as well as an effector site. RT-PCR analysis revealed that Ec-Rab7 ubiquitously expressed in all detected tissues and its transcript in spleen was up-regulated after challenge with Singapore grouper iridovirus (SGIV). Subcellular localization analysis revealed that Ec-Rab7 was distributed in the cytoplasm as spots and mostly colocalized with lysosomes. Notably, the ectopic expressed Ec-Rab7 partly aggregated into the viral factories in cells infected by SGIV. Furthermore, overexpression of Ec-Rab7 accelerated the occurrence of cytopathic effect (CPE) induced by SGIV infection and promoted viral gene transcription. In addition, far western blotting assay revealed that Ec-Rab7 might interact with viral proteins, including SGIV VP69 and VP101. Taken together, our data suggested that Ec-Rab7 might be potentially involved in SGIV replication. (C) 2013 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000331671600003
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10491
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Fu, J; Huang, YH; Cai, J; Wei, SN; Ouyang, ZL; Ye, FZ; Huang, XH; Qin, QW.Identification and characterization of Rab7 from orange-spotted grouper, Epinephelus coioides,FISH & SHELLFISH IMMUNOLOGY,2014,36(1):19-26
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