中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection
作者: Wei, SN ; Huang, YH ; Huang, XH ; Cai, J ; Yan, Y ; Guo, CY ; Qin, QW
通讯作者: qinqw@scsio.ac.cn
关键词: Epinephelus coioides ; Cathepsin B ; Singapore grouper iridovirus (SGIV) ; Virus replication ; Apoptosis
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2014
卷: 36, 期:1, 页:194-205
收录类别: sci
部门归属: [Wei, Shina ; Huang, Youhua ; Huang, Xiaohong ; Cai, Jia ; Yan, Yang ; Guo, Chuanyu ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Cai, Jia ; Guo, Chuanyu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
项目归属: LMB
资助者: National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070]; knowledge innovation program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-YW-BR-08]
摘要: The lysosomal cysteine protease cathepsin B of papain family is a key regulator and signaling molecule that involves in various biological processes, such as the regulation of apoptosis and activation of virus. In the present study, cathepsin B gene (Ec-CB) was cloned and characterized from orange-spotted grouper, Epinephelus coioides. The full-length Ec-CB cDNA was composed of 1918 bp and encoded a polypeptide of 330 amino acids with higher identities to cathepsin B of teleosts and mammalians. Ec-CB possessed typical cathepsin B structural features including an N-terminal signal peptide, the propeptide region and the cysteine protease domain which were conserved in other cathepsin B sequences. Phylogenetic analysis revealed that Ec-CB was most closely related to Lutjanus argentimaculatus. RT-PCR analysis showed that Ec-CB transcript was expressed in all the examined tissues which abundant in spleen, kidney and gill. After challenged with Singapore grouper iridovirus (SGIV) stimulation, the mRNA expression of cathepsin B in E. coioides was up-regulated at 24 h post-infection. Subcellular localization analysis revealed that Ec-CB was distributed predominantly in the cytoplasm. When the fish cells (GS or FHM) were treated with the cathepsin B specific inhibitor CA-074Me, the occurrence of CPE induced by SGIV was delayed, and the viral gene transcription was significantly inhibited. Additionally, SGIV-induced typical apoptosis was also inhibited by CA-074Me in FHM cells. Taken together, our results demonstrated that the Ec-CB might play a functional role in SGIV infection. (C) 2013 Elsevier Ltd. All rights reserved.
原文出处: 查看原文
WOS记录号: WOS:000331671600024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10493
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection.pdf(5891KB)----开放获取View Download

Recommended Citation:
Wei, SN; Huang, YH; Huang, XH; Cai, J; Yan, Y; Guo, CY; Qin, QW.Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection,FISH & SHELLFISH IMMUNOLOGY,2014,36(1):194-205
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wei, SN]'s Articles
[Huang, YH]'s Articles
[Huang, XH]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wei, SN]‘s Articles
[Huang, YH]‘s Articles
[Huang, XH]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace