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Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection
Wei, SN; Huang, YH; Huang, XH; Cai, J; Yan, Y; Guo, CY; Qin, QW; qinqw@scsio.ac.cn
2014
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume36Issue:1Pages:194-205
AbstractThe lysosomal cysteine protease cathepsin B of papain family is a key regulator and signaling molecule that involves in various biological processes, such as the regulation of apoptosis and activation of virus. In the present study, cathepsin B gene (Ec-CB) was cloned and characterized from orange-spotted grouper, Epinephelus coioides. The full-length Ec-CB cDNA was composed of 1918 bp and encoded a polypeptide of 330 amino acids with higher identities to cathepsin B of teleosts and mammalians. Ec-CB possessed typical cathepsin B structural features including an N-terminal signal peptide, the propeptide region and the cysteine protease domain which were conserved in other cathepsin B sequences. Phylogenetic analysis revealed that Ec-CB was most closely related to Lutjanus argentimaculatus. RT-PCR analysis showed that Ec-CB transcript was expressed in all the examined tissues which abundant in spleen, kidney and gill. After challenged with Singapore grouper iridovirus (SGIV) stimulation, the mRNA expression of cathepsin B in E. coioides was up-regulated at 24 h post-infection. Subcellular localization analysis revealed that Ec-CB was distributed predominantly in the cytoplasm. When the fish cells (GS or FHM) were treated with the cathepsin B specific inhibitor CA-074Me, the occurrence of CPE induced by SGIV was delayed, and the viral gene transcription was significantly inhibited. Additionally, SGIV-induced typical apoptosis was also inhibited by CA-074Me in FHM cells. Taken together, our results demonstrated that the Ec-CB might play a functional role in SGIV infection. (C) 2013 Elsevier Ltd. All rights reserved.
Department[Wei, Shina ; Huang, Youhua ; Huang, Xiaohong ; Cai, Jia ; Yan, Yang ; Guo, Chuanyu ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Cai, Jia ; Guo, Chuanyu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
KeywordEpinephelus Coioides Cathepsin b Singapore Grouper Iridovirus (Sgiv) Virus Replication Apoptosis
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationNational Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070]; knowledge innovation program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-YW-BR-08] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070]; knowledge innovation program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-YW-BR-08] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070]; knowledge innovation program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-YW-BR-08] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070]; knowledge innovation program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-YW-BR-08]
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Indexed Bysci
Funding ProjectLMB
Funding OrganizationNational Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070]; knowledge innovation program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-YW-BR-08] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070]; knowledge innovation program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-YW-BR-08] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070]; knowledge innovation program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-YW-BR-08] ; National Basic Research Program of China (973) [2012CB114402]; Natural Science Foundation of China [31330082, 30930070]; knowledge innovation program of the Chinese Academy of Sciences [KZCX2-EW-Q213, KZCX2-YW-BR-08]
WOS IDWOS:000331671600024
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10493
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorqinqw@scsio.ac.cn
Recommended Citation
GB/T 7714
Wei, SN,Huang, YH,Huang, XH,et al. Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection[J]. FISH & SHELLFISH IMMUNOLOGY,2014,36(1):194-205.
APA Wei, SN.,Huang, YH.,Huang, XH.,Cai, J.,Yan, Y.,...&qinqw@scsio.ac.cn.(2014).Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection.FISH & SHELLFISH IMMUNOLOGY,36(1),194-205.
MLA Wei, SN,et al."Characterization of cathepsin B gene from orange-spotted grouper, Epinephelus coioides involved in SGIV infection".FISH & SHELLFISH IMMUNOLOGY 36.1(2014):194-205.
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