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学科主题: Biodiversity & Conservation ; Genetics & Heredity
题名: Development of SNP markers in Crassostrea hongkongensis based on the next-generation sequencing and high resolution melting analysis
作者: Huang, JM ; Zhang, Y ; Li, J ; Yu, ZN
通讯作者: carlzyu@scsio.ac.cn
关键词: C. hongkongensis ; SNP markers ; Transcriptome sequencing ; High resolution melting
刊名: CONSERVATION GENETICS RESOURCES
发表日期: 2014
卷: 6, 期:3, 页:559-562
收录类别: sci
部门归属: [Huang, Jiaomei ; Zhang, Yang ; Li, Jun ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Huang, Jiaomei] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
项目归属: LTO
资助者: National High-Tech Research and Development Program of China [2012AA10A405-3]
摘要: Novel single nucleotide polymorphisms (SNPs) were screened using next generation sequencing in Hong Kong oyster, Crassostrea hongkongensis. A total of 91,980 putative SNPs were identified through de novo assembly of the 454 transcriptome database, and 83 of these were selected to validate polymorphism. Using high resolution melting analysis, 17 SNP markers were developed successfully with 48 individuals from two wild populations from Shantou and Zhuhai, China. The observed heterozygosities ranged from 0.125 to 0.667 and 0 to 0.708 in the two populations, respectively, while the expected ones ranged from 0.311 to 0.511 and 0.120 to 0.511 in the two samples, respectively. Fifteen SNPs have been confirmed to fit Hardy-Weinberg Equilibrium after Bonferroni correction (p < 0.003) in both populations. To our knowledge, this is the first set of SNP markers developed in C. hongkongensis, which would provide valuable information in facilitating genetics and breeding studies in the future.
原文出处: 查看原文
WOS记录号: WOS:000340404900016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10511
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Huang, JM; Zhang, Y; Li, J; Yu, ZN.Development of SNP markers in Crassostrea hongkongensis based on the next-generation sequencing and high resolution melting analysis,CONSERVATION GENETICS RESOURCES,2014,6(3):559-562
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