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学科主题: Biochemistry & Molecular Biology ; Toxicology
题名: Characterization and Functional Analysis of Voltage-Dependent Anion Channel 1 (VDAC1) from Orange-Spotted Grouper (Epinephelus coioides)
作者: Shi, Y ; Zhao, Z ; Hong, XY ; Chen, KC ; Zhu, XP
通讯作者: zhuxinping_1964@163.com
关键词: VDAC1 ; Epinephelus coioides ; Tissue Expression ; Subcellular Localization ; Overexpression
刊名: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
发表日期: 2014
卷: 28, 期:7, 页:292-301
收录类别: sci
部门归属: [Shi, Yan ; Hong, Xiaoyou ; Chen, Kunci ; Zhu, Xinping] Chinese Acad Fishery Sci, Minist Agr, Pearl River Fishery Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China ; [Zhao, Zhe] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
项目归属: LTO
资助者: Project of Guangzhou City for Pearl River Science & Technology Star [2012J2200091, 2013J2200094]; Project of Science and Technology of Guangdong Province [2012B020307003, 2012B020308005]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]
摘要: The voltage-dependent anion channel (VDAC) is a highly conserved integral protein of mitochondria in different eukaryotic species. It forms a selective channel in the mitochondrial outer membrane that serves as the controlled pathway for small metabolites and ions. In this study, a VDAC gene, EcVDAC1, was isolated from orange-spotted grouper (Epinephelus coioides). The EcVDAC1 exhibits ubiquitous expression in various tissues of orange-spotted grouper and is upregulated in liver, gill, and spleen after stimulation with lipopolysaccharides (LPS). Subcellular localization analysis shows that the EcVDAC1 protein colocalized with the mitochondria. A caspase-3 assay demonstrates that overexpression of the EcVDAC1 induced apoptotic cell death in fathead minnow cells. The data presented in this study provide new information regarding the relationship between LPS and the EcVDAC1 gene, suggesting that the fish VDAC1 gene may play an important role in antibacterial immune response. (C) 2014 Wiley Periodicals, Inc.
原文出处: 查看原文
WOS记录号: WOS:000339723300003
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10533
Appears in Collections:热带海洋环境国家重点实验室(LTO)_期刊论文

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Shi, Y; Zhao, Z; Hong, XY; Chen, KC; Zhu, XP.Characterization and Functional Analysis of Voltage-Dependent Anion Channel 1 (VDAC1) from Orange-Spotted Grouper (Epinephelus coioides),JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,2014,28(7):292-301
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