Chinese | English
SCSIO OpenIR  > 热带海洋环境国家重点实验室(LTO)
Characterization and Functional Analysis of Voltage-Dependent Anion Channel 1 (VDAC1) from Orange-Spotted Grouper (Epinephelus coioides)
Shi, Y; Zhao, Z; Hong, XY; Chen, KC; Zhu, XP; zhuxinping_1964@163.com
2014
Source PublicationJOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
ISSN1095-6670
Volume28Issue:7Pages:292-301
AbstractThe voltage-dependent anion channel (VDAC) is a highly conserved integral protein of mitochondria in different eukaryotic species. It forms a selective channel in the mitochondrial outer membrane that serves as the controlled pathway for small metabolites and ions. In this study, a VDAC gene, EcVDAC1, was isolated from orange-spotted grouper (Epinephelus coioides). The EcVDAC1 exhibits ubiquitous expression in various tissues of orange-spotted grouper and is upregulated in liver, gill, and spleen after stimulation with lipopolysaccharides (LPS). Subcellular localization analysis shows that the EcVDAC1 protein colocalized with the mitochondria. A caspase-3 assay demonstrates that overexpression of the EcVDAC1 induced apoptotic cell death in fathead minnow cells. The data presented in this study provide new information regarding the relationship between LPS and the EcVDAC1 gene, suggesting that the fish VDAC1 gene may play an important role in antibacterial immune response. (C) 2014 Wiley Periodicals, Inc.
Department[Shi, Yan ; Hong, Xiaoyou ; Chen, Kunci ; Zhu, Xinping] Chinese Acad Fishery Sci, Minist Agr, Pearl River Fishery Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China ; [Zhao, Zhe] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
KeywordVdac1 Epinephelus Coioides Tissue Expression Subcellular Localization Overexpression
Subject AreaBiochemistry & Molecular Biology ; Toxicology
Funding OrganizationProject of Guangzhou City for Pearl River Science & Technology Star [2012J2200091, 2013J2200094]; Project of Science and Technology of Guangdong Province [2012B020307003, 2012B020308005]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09] ; Project of Guangzhou City for Pearl River Science & Technology Star [2012J2200091, 2013J2200094]; Project of Science and Technology of Guangdong Province [2012B020307003, 2012B020308005]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09] ; Project of Guangzhou City for Pearl River Science & Technology Star [2012J2200091, 2013J2200094]; Project of Science and Technology of Guangdong Province [2012B020307003, 2012B020308005]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09] ; Project of Guangzhou City for Pearl River Science & Technology Star [2012J2200091, 2013J2200094]; Project of Science and Technology of Guangdong Province [2012B020307003, 2012B020308005]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]
URL查看原文
Indexed Bysci
Funding ProjectLTO
Funding OrganizationProject of Guangzhou City for Pearl River Science & Technology Star [2012J2200091, 2013J2200094]; Project of Science and Technology of Guangdong Province [2012B020307003, 2012B020308005]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09] ; Project of Guangzhou City for Pearl River Science & Technology Star [2012J2200091, 2013J2200094]; Project of Science and Technology of Guangdong Province [2012B020307003, 2012B020308005]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09] ; Project of Guangzhou City for Pearl River Science & Technology Star [2012J2200091, 2013J2200094]; Project of Science and Technology of Guangdong Province [2012B020307003, 2012B020308005]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09] ; Project of Guangzhou City for Pearl River Science & Technology Star [2012J2200091, 2013J2200094]; Project of Science and Technology of Guangdong Province [2012B020307003, 2012B020308005]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]
WOS IDWOS:000339723300003
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10533
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorzhuxinping_1964@163.com
Recommended Citation
GB/T 7714		 Shi, Y,Zhao, Z,Hong, XY,et al. Characterization and Functional Analysis of Voltage-Dependent Anion Channel 1 (VDAC1) from Orange-Spotted Grouper (Epinephelus coioides)[J]. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,2014,28(7):292-301.
APA Shi, Y,Zhao, Z,Hong, XY,Chen, KC,Zhu, XP,&zhuxinping_1964@163.com.(2014).Characterization and Functional Analysis of Voltage-Dependent Anion Channel 1 (VDAC1) from Orange-Spotted Grouper (Epinephelus coioides).JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY,28(7),292-301.
MLA Shi, Y,et al."Characterization and Functional Analysis of Voltage-Dependent Anion Channel 1 (VDAC1) from Orange-Spotted Grouper (Epinephelus coioides)".JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY 28.7(2014):292-301.
Files in This Item:
File Name/Size DocType Version Access License
Characterization and（1227KB） 开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.