A novel ortholog of serum response factor (SRF) with immune defense function identified in Crassostrea hongkongensis
Xiang, ZM; Qu, FF; Qi, L; Zhang, Y; Xiao, S; Yu, ZN; zhimingxiang@scsio.ac.cn; carlzyu@scsio.ac.cn
2014
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号36期号:1页码:75-82
摘要Serum response factor (SRF) function is essential for transcriptional regulation of numerous growth-factor-inducible genes and triggers proliferation, differentiation and apoptosis of the cells. In this report, the first mollusk serum response factor like homolog gene (designated ChSRF) was identified and characterized from the Hong Kong oyster, Crassostrea hongkongensis. The full-length cDNA of ChSRF was 1716 bp in length and encodes a putative protein of 434 amino acids respectively, and shares the MADS domain at the N-terminal. ChSRF is ubiquitously expressed in various tissues, with the highest expression level observed in muscle. Temporal expression of ChSRF following microbe infection shows that the expression of ChSRF in hemocytes increases from 3 to 24 h post-challenge. As a target gene of SRF, beta-actin demonstrates a similar gene expression mode in constitutive tissue and pathogen infection. Furthermore, some protein profiles of ChSRF was revealed, fluorescence microscopy results show that ChSRF located in the nuclei of HeLa cells and over-expression of ChSRF activated the transcriptional activities of MAPK signal pathway in HEK293T cells. These results indicate that ChSRF maybe play an important role in signal transduction in the immunity and development response of oysters. Furthermore, we found that ChSRF could regulate the expression of beta-actin gene, which indicate that ChSRF is a muscle differentiation regulator in the oyster and it will help us to improve aquaculture production. (C) 2013 Elsevier Ltd. All rights reserved.
部门归属[Xiang, Zhiming ; Qu, Fufa ; Zhang, Yang ; Xiao, Shu ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Qu, Fufa] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Qi, Lin] Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China
关键词Crassostrea Hongkongensis Chsrf Mapk Signal Pathway
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者National Science Foundation of China [31202021, 31001130, 41176150]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [31202021, 31001130, 41176150]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [31202021, 31001130, 41176150]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [31202021, 31001130, 41176150]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095]
URL查看原文
收录类别sci
资助项目LTO
资助者National Science Foundation of China [31202021, 31001130, 41176150]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [31202021, 31001130, 41176150]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [31202021, 31001130, 41176150]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095] ; National Science Foundation of China [31202021, 31001130, 41176150]; National Basic Research Program of China [2010CB126404]; Program of the Pearl River Young Talents of Science and Technology in Guangzhou, China [2013J2200095]
WOS记录号WOS:000331671600010
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10589
专题热带海洋环境国家重点实验室(LTO)
通讯作者zhimingxiang@scsio.ac.cn; carlzyu@scsio.ac.cn
推荐引用方式
GB/T 7714
Xiang, ZM,Qu, FF,Qi, L,et al. A novel ortholog of serum response factor (SRF) with immune defense function identified in Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2014,36(1):75-82.
APA Xiang, ZM.,Qu, FF.,Qi, L.,Zhang, Y.,Xiao, S.,...&carlzyu@scsio.ac.cn.(2014).A novel ortholog of serum response factor (SRF) with immune defense function identified in Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,36(1),75-82.
MLA Xiang, ZM,et al."A novel ortholog of serum response factor (SRF) with immune defense function identified in Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 36.1(2014):75-82.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
A novel ortholog of (3282KB) 开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。