Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China
Wu, P; Wang, YS; Sun, FL; Wu, ML; Peng, YL; yswang@scsio.ac.cn
2014
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
卷号98期号:2页码:875-884
摘要Bacterial community compositions were characterized using denaturing gradient gel electrophoresis analysis of bacterial 16S rRNA gene in the sediments of the Pearl River estuary. Sequencing analyses of the excised bands indicated that Gram-negative bacteria, especially Gammaproteobacteria, were dominant in the Pearl River estuary. The diversity of polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHD) gene in this estuary was then assessed by clone library analysis. The phylogenetic analyses showed that all PAH-RHD gene sequences of Gram-negative bacteria (PAH-RHD[GN]) were closely related to the nagAc gene described for Ralstonia sp. U2 or nahAc gene for Pseudomonas sp. 9816-4, while the PAH-RHD gene sequences of Gram-positive bacteria (PAH-RHD[GP]) at sampling site A1 showed high sequence similarity to the nidA gene from Mycobacterium species. Meanwhile, molecular diversity of the two functional genes was higher at the upstream of this region, while lower at the downstream. Redundancy analysis indicated that environmental factors, such as NH4-N, aPAHs, pH, SiO3-Si, and water depth, affected the distribution of the PAH-RHD[GN] gene in the Pearl River estuary.
部门归属[Wu, Peng ; Wang, You-Shao ; Sun, Fu-Lin ; Wu, Mei-Lin ; Peng, Ya-lan] Chinese Acad Sci, South China Sea Inst Oceanol, State Key Lab Trop Oceanog, Guangzhou 510301, Guangdong, Peoples R China ; [Wu, Peng ; Wang, You-Shao ; Sun, Fu-Lin ; Wu, Mei-Lin ; Peng, Ya-lan] Chinese Acad Sci, Daya Bay Marine Biol Res Stn, Shenzhen 518121, Peoples R China
关键词Polycyclic Aromatic Hydrocarbons Ring-hydroxylating Dioxygenase The Pearl River Estuary Bacterial Community
学科领域Biotechnology & Applied Microbiology
资助者Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402]
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收录类别sci
资助项目LTO
资助者Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402]
WOS记录号WOS:000329627600035
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10594
专题热带海洋环境国家重点实验室(LTO)
通讯作者yswang@scsio.ac.cn
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GB/T 7714
Wu, P,Wang, YS,Sun, FL,et al. Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(2):875-884.
APA Wu, P,Wang, YS,Sun, FL,Wu, ML,Peng, YL,&yswang@scsio.ac.cn.(2014).Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(2),875-884.
MLA Wu, P,et al."Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.2(2014):875-884.
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