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Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China
Wu, P; Wang, YS; Sun, FL; Wu, ML; Peng, YL; yswang@scsio.ac.cn
2014
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
Volume98Issue:2Pages:875-884
AbstractBacterial community compositions were characterized using denaturing gradient gel electrophoresis analysis of bacterial 16S rRNA gene in the sediments of the Pearl River estuary. Sequencing analyses of the excised bands indicated that Gram-negative bacteria, especially Gammaproteobacteria, were dominant in the Pearl River estuary. The diversity of polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHD) gene in this estuary was then assessed by clone library analysis. The phylogenetic analyses showed that all PAH-RHD gene sequences of Gram-negative bacteria (PAH-RHD[GN]) were closely related to the nagAc gene described for Ralstonia sp. U2 or nahAc gene for Pseudomonas sp. 9816-4, while the PAH-RHD gene sequences of Gram-positive bacteria (PAH-RHD[GP]) at sampling site A1 showed high sequence similarity to the nidA gene from Mycobacterium species. Meanwhile, molecular diversity of the two functional genes was higher at the upstream of this region, while lower at the downstream. Redundancy analysis indicated that environmental factors, such as NH4-N, aPAHs, pH, SiO3-Si, and water depth, affected the distribution of the PAH-RHD[GN] gene in the Pearl River estuary.
Department[Wu, Peng ; Wang, You-Shao ; Sun, Fu-Lin ; Wu, Mei-Lin ; Peng, Ya-lan] Chinese Acad Sci, South China Sea Inst Oceanol, State Key Lab Trop Oceanog, Guangzhou 510301, Guangdong, Peoples R China ; [Wu, Peng ; Wang, You-Shao ; Sun, Fu-Lin ; Wu, Mei-Lin ; Peng, Ya-lan] Chinese Acad Sci, Daya Bay Marine Biol Res Stn, Shenzhen 518121, Peoples R China
KeywordPolycyclic Aromatic Hydrocarbons Ring-hydroxylating Dioxygenase The Pearl River Estuary Bacterial Community
Subject AreaBiotechnology & Applied Microbiology
Funding OrganizationKnowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402]
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Funding ProjectLTO
Funding OrganizationKnowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402] ; Knowledge Innovation Programs of the Chinese Academy of Sciences [KSCX2-EW-G-12C, KSCX2-SW-132]; National Natural Science Foundation of China [41076070, 41176101]; key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period [2009BADB2B0606, 2012BAC07B0402]
WOS IDWOS:000329627600035
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10594
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authoryswang@scsio.ac.cn
Recommended Citation
GB/T 7714
Wu, P,Wang, YS,Sun, FL,et al. Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(2):875-884.
APA Wu, P,Wang, YS,Sun, FL,Wu, ML,Peng, YL,&yswang@scsio.ac.cn.(2014).Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(2),875-884.
MLA Wu, P,et al."Bacterial polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenases in the sediments from the Pearl River estuary, China".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.2(2014):875-884.
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