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Characterization and expression analysis of three CBF/DREB1 transcriptional factor genes from mangrove Avicennia marina
[Peng, Ya-Lan; Wang, You-Shao; Cheng, Hao; Sun, Cui-Ci; Wu, Peng; Wang, Li-Ying; Fei, Jiao] Chinese Acad Sci, South China Sea Inst Oceanol, State Key Lab Trop Oceanog, Guangzhou 510301, Guangdong, Peoples R China; [Wang, You-Shao; Sun, Cui-Ci] Chinese Acad Sci, Daya Bay Marine Biol Res Stn, Shenzhen 518121, Peoples R China; yswang@scsio.ac.cn
2013
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume140Pages:68-76
AbstractThree CBF/DREB1 (C-repeat binding factor/dehydration responsive element-binding factor 1) homologues were isolated from mangrove Avicennia marina and designated AmCBF1, 2 and 3. Multiple sequence analysis showed that the three deduced proteins all contain an AP2 DNA-binding domain and two CBF signature sequences. According to the phylogenetic analysis, these proteins belong to the A-1 subgroup of the DREB subfamily. Expression analyses based on quantitative real-time PCR revealed that the AmCBF2 displayed relatively high expression under normal conditions, with the highest level in stems, while both AmCBF1 and 3 were weakly expressed without stress. The three genes also showed different responses to various environmental stimuli. The AmCBF2 was inducible by cold, drought, high salinity, heavy metals, as well as abscisic acid (ABA), and exhibited much stronger induction by cold, drought, Pb2+ or Zn2+ than by NaCl, ABA or Cd2+. In contrast, both AmCBF1 and AmCBF3 displayed insignificant changes under these stimuli. These results indicate that the three AmCBF genes play different roles in A. marina and the AmCBF2 might be involved in the signaling pathway of cold, drought and heavy metal stress response. (C) 2013 Elsevier B.V. All rights reserved.
DepartmentLTO
KeywordAbiotic Stress Avicennia Marina Cbf/dreb1 Transcription Factor Gene Expression Mangrove Real-time Quantitative Pcr
Subject AreaMarine & Freshwater Biology ; Toxicology
Funding OrganizationThis research was supported by the National Natural Science Foundation of China (Nos. 41176101 and 41076070), the projects of knowledge innovation program of Chinese Academy of Sciences (KSCX2-YW-Z-1024) and the key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (No. 2012BAC07B0402 and 2009BADB2B06). ; This research was supported by the National Natural Science Foundation of China (Nos. 41176101 and 41076070), the projects of knowledge innovation program of Chinese Academy of Sciences (KSCX2-YW-Z-1024) and the key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (No. 2012BAC07B0402 and 2009BADB2B06). ; This research was supported by the National Natural Science Foundation of China (Nos. 41176101 and 41076070), the projects of knowledge innovation program of Chinese Academy of Sciences (KSCX2-YW-Z-1024) and the key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (No. 2012BAC07B0402 and 2009BADB2B06). ; This research was supported by the National Natural Science Foundation of China (Nos. 41176101 and 41076070), the projects of knowledge innovation program of Chinese Academy of Sciences (KSCX2-YW-Z-1024) and the key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (No. 2012BAC07B0402 and 2009BADB2B06).
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Language英语
Funding OrganizationThis research was supported by the National Natural Science Foundation of China (Nos. 41176101 and 41076070), the projects of knowledge innovation program of Chinese Academy of Sciences (KSCX2-YW-Z-1024) and the key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (No. 2012BAC07B0402 and 2009BADB2B06). ; This research was supported by the National Natural Science Foundation of China (Nos. 41176101 and 41076070), the projects of knowledge innovation program of Chinese Academy of Sciences (KSCX2-YW-Z-1024) and the key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (No. 2012BAC07B0402 and 2009BADB2B06). ; This research was supported by the National Natural Science Foundation of China (Nos. 41176101 and 41076070), the projects of knowledge innovation program of Chinese Academy of Sciences (KSCX2-YW-Z-1024) and the key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (No. 2012BAC07B0402 and 2009BADB2B06). ; This research was supported by the National Natural Science Foundation of China (Nos. 41176101 and 41076070), the projects of knowledge innovation program of Chinese Academy of Sciences (KSCX2-YW-Z-1024) and the key projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (No. 2012BAC07B0402 and 2009BADB2B06).
WOS IDWOS:000324900800008
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10882
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authoryswang@scsio.ac.cn
Recommended Citation
GB/T 7714
[Peng, Ya-Lan,Wang, You-Shao,Cheng, Hao,et al. Characterization and expression analysis of three CBF/DREB1 transcriptional factor genes from mangrove Avicennia marina[J]. AQUATIC TOXICOLOGY,2013,140:68-76.
APA [Peng, Ya-Lan.,Wang, You-Shao.,Cheng, Hao.,Sun, Cui-Ci.,Wu, Peng.,...&yswang@scsio.ac.cn.(2013).Characterization and expression analysis of three CBF/DREB1 transcriptional factor genes from mangrove Avicennia marina.AQUATIC TOXICOLOGY,140,68-76.
MLA [Peng, Ya-Lan,et al."Characterization and expression analysis of three CBF/DREB1 transcriptional factor genes from mangrove Avicennia marina".AQUATIC TOXICOLOGY 140(2013):68-76.
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