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学科主题: Food Science & Technology
题名: Real-time PCR targeting OmpA gene for detection of Cronobacter spp. in powdered infant formula
作者: [Dong, Xiaohui ; Wu, Qingping ; Wu, Kui] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China ; [Dong, Xiaohui ; Wu, Qingping ; Wu, Kui ; Zhang, Jumei] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangdong Open Lab Appl Microbiol, State Key Lab Appl Microbiol, Guangzhou 510070, Guangdong, Peoples R China ; [Dong, Xiaohui ; Wu, Kui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者: wuqp203@yahoo.com.cn
关键词: Cronobacter spp. ; real-time PCR ; TaqMan ; ompA ; detection
刊名: FOOD SCIENCE AND BIOTECHNOLOGY
发表日期: 2013
卷: 22, 期:2, 页:309-313
部门归属: LTO
资助者: We acknowledge the financing support of the Guangdong-Hongkong Major Breakthrough Project in Key Fields (2010498D10008) and Science and Technology Planning Project of Guangzhou (2010u1-E00611).
摘要: Cronobacter spp. (formerly Enterobacter sakazakii), a pathogen commonly found in powdered infant formula (PIF), is a rare cause of invasive infection with a high mortality rate in neonates. In the present study, a realtime PCR assay was conducted to identify the pathogens in PIF using a TaqMan probe targeting the outer membrane protein A gene (ompA) of Cronobacter spp. The specificity of the PCR assay was tested against 25 strains of Cronobacter spp. and 38 non-Cronobacter bacterial species. The detection limits of this method are 1.0x10(2) copy/mu L in standard plasmid, 1.1 CFU/100 g in PIF through 38 h of enrichment, and 2.8x10(2) CFU/mL in phosphate-buffered saline (pH 7.0). Based on the detection limits, real-time PCR is more sensitive than simplex and duplex PCR. These methods were successfully applied to actual samples, indicating that this real-time PCR assay can be used for the detection of Cronobacter spp. in PIF.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000318411900003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10920
Appears in Collections:热带海洋环境国家重点实验室(LTO)_期刊论文

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Recommended Citation:
[Dong, Xiaohui; Wu, Qingping; Wu, Kui] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Dong, Xiaohui; Wu, Qingping; Wu, Kui; Zhang, Jumei] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangdong Open Lab Appl Microbiol, State Key Lab Appl Microbiol, Guangzhou 510070, Guangdong, Peoples R China; [Dong, Xiaohui; Wu, Kui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.Real-time PCR targeting OmpA gene for detection of Cronobacter spp. in powdered infant formula,FOOD SCIENCE AND BIOTECHNOLOGY,2013,22(2):309-313
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