Real-time PCR targeting OmpA gene for detection of Cronobacter spp. in powdered infant formula
[Dong, Xiaohui; Wu, Qingping; Wu, Kui] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; Wu, Kui; Zhang, Jumei] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangdong Open Lab Appl Microbiol, State Key Lab Appl Microbiol, Guangzhou 510070, Guangdong, Peoples R China; Wu, Kui] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; wuqp203@yahoo.com.cn
2013
发表期刊FOOD SCIENCE AND BIOTECHNOLOGY
ISSN1226-7708
卷号22期号:2页码:309-313
摘要Cronobacter spp. (formerly Enterobacter sakazakii), a pathogen commonly found in powdered infant formula (PIF), is a rare cause of invasive infection with a high mortality rate in neonates. In the present study, a realtime PCR assay was conducted to identify the pathogens in PIF using a TaqMan probe targeting the outer membrane protein A gene (ompA) of Cronobacter spp. The specificity of the PCR assay was tested against 25 strains of Cronobacter spp. and 38 non-Cronobacter bacterial species. The detection limits of this method are 1.0x10(2) copy/mu L in standard plasmid, 1.1 CFU/100 g in PIF through 38 h of enrichment, and 2.8x10(2) CFU/mL in phosphate-buffered saline (pH 7.0). Based on the detection limits, real-time PCR is more sensitive than simplex and duplex PCR. These methods were successfully applied to actual samples, indicating that this real-time PCR assay can be used for the detection of Cronobacter spp. in PIF.
部门归属LTO
关键词Cronobacter Spp. Real-time Pcr Taqman Ompa Detection
学科领域Food Science & Technology
资助者We acknowledge the financing support of the Guangdong-Hongkong Major Breakthrough Project in Key Fields (2010498D10008) and Science and Technology Planning Project of Guangzhou (2010u1-E00611). ; We acknowledge the financing support of the Guangdong-Hongkong Major Breakthrough Project in Key Fields (2010498D10008) and Science and Technology Planning Project of Guangzhou (2010u1-E00611). ; We acknowledge the financing support of the Guangdong-Hongkong Major Breakthrough Project in Key Fields (2010498D10008) and Science and Technology Planning Project of Guangzhou (2010u1-E00611). ; We acknowledge the financing support of the Guangdong-Hongkong Major Breakthrough Project in Key Fields (2010498D10008) and Science and Technology Planning Project of Guangzhou (2010u1-E00611).
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语种英语
资助者We acknowledge the financing support of the Guangdong-Hongkong Major Breakthrough Project in Key Fields (2010498D10008) and Science and Technology Planning Project of Guangzhou (2010u1-E00611). ; We acknowledge the financing support of the Guangdong-Hongkong Major Breakthrough Project in Key Fields (2010498D10008) and Science and Technology Planning Project of Guangzhou (2010u1-E00611). ; We acknowledge the financing support of the Guangdong-Hongkong Major Breakthrough Project in Key Fields (2010498D10008) and Science and Technology Planning Project of Guangzhou (2010u1-E00611). ; We acknowledge the financing support of the Guangdong-Hongkong Major Breakthrough Project in Key Fields (2010498D10008) and Science and Technology Planning Project of Guangzhou (2010u1-E00611).
WOS记录号WOS:000318411900003
引用统计
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10920
专题热带海洋环境国家重点实验室(LTO)
通讯作者wuqp203@yahoo.com.cn
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[Dong, Xiaohui,Wu, Qingping,Wu, Kui] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China,et al. Real-time PCR targeting OmpA gene for detection of Cronobacter spp. in powdered infant formula[J]. FOOD SCIENCE AND BIOTECHNOLOGY,2013,22(2):309-313.
APA [Dong, Xiaohui.,Wu, Qingping.,Wu, Kui] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.,Wu, Kui.,Zhang, Jumei] Guangdong Inst Microbiol, Guangdong Prov Key Lab Microbial Culture Collect, Guangdong Open Lab Appl Microbiol, State Key Lab Appl Microbiol, Guangzhou 510070, Guangdong, Peoples R China.,...&wuqp203@yahoo.com.cn.(2013).Real-time PCR targeting OmpA gene for detection of Cronobacter spp. in powdered infant formula.FOOD SCIENCE AND BIOTECHNOLOGY,22(2),309-313.
MLA [Dong, Xiaohui,et al."Real-time PCR targeting OmpA gene for detection of Cronobacter spp. in powdered infant formula".FOOD SCIENCE AND BIOTECHNOLOGY 22.2(2013):309-313.
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