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学科主题: Chemistry, Multidisciplinary
题名: Insights into Caerulomycin A Biosynthesis: A Two-Component Monooxygenase CrmH-Catalyzed Oxime Formation
其他题名: mannual of IR1
作者: [Zhu, Yiguang ; Zhang, Qingbo ; Li, Sumei ; Lin, Qinheng ; Zhang, Guangtao ; Zhang, Haibo ; Zhang, Changsheng] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China ; [Zhang, Qingbo ; Lin, Qinheng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Fu, Peng ; Zhu, Weiming] Ocean Univ China, Sch Med & Pharm, Key Lab Marine Drugs, Chinese Minist Educ, Qingdao 266003, Peoples R China ; [Shi, Rong] Univ Laval, Dept Biochim Microbiol & Bioinformat, PROTEO, Quebec City, PQ G1V 0A6, Canada ; [Shi, Rong] Univ Laval, IBIS, Quebec City, PQ G1V 0A6, Canada
通讯作者: weimingzhu@ouc.edu.cn ; czhang2006@gmail.com
刊名: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
发表日期: 2013
卷: 135, 期:50, 页:18750-18753
收录类别: sci
合作性质: 填入合作性质等
部门归属: LMB
项目归属: description.project
产权排名: 产权排序
资助者: Financial support was provided by MOST (2010CB833805), NSFC (31125001 and 31070045), and CAS (KZCX2-YW-JC202, KSCX2-EW-G-12, 08SL111002). We thank Profs. Yi Tang (UCLA), Shu-Ming Li (Philipps Universitat Marburg), and Shuangjun Lin (Shanghai Jiao Tong University) for help discussions. We are grateful to the analytical facilities in SCSIO.
摘要: The immunosuppressive agent caerulomycin A features a unique 2,2'-bipyridine core structure and an unusual oxime functionality. Genetic and biochemical evidence confirms that the oxime formation in caerulomycin A biosynthesis is catalyzed by CrmH, a flavin-dependent two-component monooxygenase that is compatible with multiple flavin reductases, from a primary amine via a N-hydroxylamine intermediate. Structure homologue-guided site-directed mutagenesis studies identify four amino acid residues that are essential for CrmH catalysis. This study provides the first biochemical evidence of a two-component monooxygenase that catalyzes oxime formation.
英文摘要: description.abstract
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000328865100012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10944
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
[Zhu, Yiguang; Zhang, Qingbo; Li, Sumei; Lin, Qinheng; Zhang, Guangtao; Zhang, Haibo; Zhang, Changsheng] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Qingbo; Lin, Qinheng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Fu, Peng; Zhu, Weiming] Ocean Univ China, Sch Med & Pharm, Key Lab Marine Drugs, Chinese Minist Educ, Qingdao 266003, Peoples R China; [Shi, Rong] Univ Laval, Dept Biochim Microbiol & Bioinformat, PROTEO, Quebec City, PQ G1V 0A6, Canada; [Shi, Rong] Univ Laval, IBIS, Quebec City, PQ G1V 0A6, Canada.Insights into Caerulomycin A Biosynthesis: A Two-Component Monooxygenase CrmH-Catalyzed Oxime Formation,JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(50):18750-18753
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