Characterization of an envelope gene VP19 from Singapore grouper iridovirus
其他题名mannual of IR1
[Huang, Xiaohong; Huang, Youhua; Ouyang, Zhengliang; Wang, Shaowen; Chen, Xiuli; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Gong, Jie] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China; qinqw@scsio.ac.cn
2013
发表期刊VIROLOGY JOURNAL
ISSN1743-422X
卷号10页码:-354
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摘要Background: Viral envelope proteins are always proposed to exert important function during virus infection and replication. Vertebrate iridoviruses are enveloped large DNA virus, which can cause great economic losses in aquaculture and ecological destruction. Although numerous iridovirus envelope proteins have been identified using bioinformatics and proteomic methods, their roles in virus infection remained largely unknown. Methods: Using SMART and TMHMM programs, we investigated the structural characteristics of Singapore grouper iridovirus (SGIV) VP19. A specific antibody against VP19 was generated and the expression profile of VP19 was clarified. The subcellular localization of VP19 in the absence or presence of other viral products was determined via transfection and immune fluorescence assay. In addition, Western blot assay and electron microscopy examination were performed to demonstrate whether SGIV VP19 was an envelope protein or a capsid protein. Results: Here, SGIV VP19 was cloned and characterized. Among all sequenced iridoviruses, VP19 and its orthologues shared common features, including 19 invariant cysteines, a proline-rich motif and a predicted transmembrane domain. Subsequently, the protein synthesis of VP19 was only detected at the late stage of SGIV infection and inhibited obviously by treating with AraC, confirming that VP19 was a late expressed protein. Ectopic expression of EGFP-VP19 in vitro displayed a punctate pattern in the cytoplasm. In SGIV infected cells, the newly synthesized VP19 protein was initially localized in the cytoplasm in a punctate pattern, and then aggregated into the virus assembly site at the late stage of SGIV infection, suggesting that other viral protein products were essential for VP19's function during SGIV infection. In addition, Western blot assay and electron microscopy observation revealed that SGIV VP19 was associated with viral envelope, which was different from major capsid protein (MCP). Conclusion: Taken together, the current data suggested that VP19 represented a conserved envelope protein in iridovirus, and might contribute greatly to virus assembly during virus infection.; description.abstract
部门归属LMB
关键词Sgiv Iridovirus Vp19 Envelope Protein Virus Assembly
学科领域Virology
资助者This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114406), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-12B) and the National High-Tech Research and. Development Program (863) (SS2014AA091607). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114406), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-12B) and the National High-Tech Research and. Development Program (863) (SS2014AA091607). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114406), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-12B) and the National High-Tech Research and. Development Program (863) (SS2014AA091607). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114406), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-12B) and the National High-Tech Research and. Development Program (863) (SS2014AA091607).
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收录类别sci
语种英语
资助项目description.project
资助者This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114406), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-12B) and the National High-Tech Research and. Development Program (863) (SS2014AA091607). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114406), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-12B) and the National High-Tech Research and. Development Program (863) (SS2014AA091607). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114406), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-12B) and the National High-Tech Research and. Development Program (863) (SS2014AA091607). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114406), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-12B) and the National High-Tech Research and. Development Program (863) (SS2014AA091607).
WOS记录号WOS:000330051600001
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10945
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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[Huang, Xiaohong,Huang, Youhua,Ouyang, Zhengliang,et al. Characterization of an envelope gene VP19 from Singapore grouper iridovirus[J]. VIROLOGY JOURNAL,2013,10:-354.
APA [Huang, Xiaohong.,Huang, Youhua.,Ouyang, Zhengliang.,Wang, Shaowen.,Chen, Xiuli.,...&qinqw@scsio.ac.cn.(2013).Characterization of an envelope gene VP19 from Singapore grouper iridovirus.VIROLOGY JOURNAL,10,-354.
MLA [Huang, Xiaohong,et al."Characterization of an envelope gene VP19 from Singapore grouper iridovirus".VIROLOGY JOURNAL 10(2013):-354.
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