中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Virology
题名: Characterization of an envelope gene VP19 from Singapore grouper iridovirus
其他题名: mannual of IR1
作者: [Huang, Xiaohong ; Huang, Youhua ; Ouyang, Zhengliang ; Wang, Shaowen ; Chen, Xiuli ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Gong, Jie] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
通讯作者: qinqw@scsio.ac.cn
关键词: SGIV ; Iridovirus ; VP19 ; Envelope protein ; Virus assembly
刊名: VIROLOGY JOURNAL
发表日期: 2013
卷: 10, 页:-354
收录类别: sci
合作性质: 填入合作性质等
部门归属: LMB
项目归属: description.project
产权排名: 产权排序
资助者: This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114406), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-12B) and the National High-Tech Research and. Development Program (863) (SS2014AA091607).
摘要: Background: Viral envelope proteins are always proposed to exert important function during virus infection and replication. Vertebrate iridoviruses are enveloped large DNA virus, which can cause great economic losses in aquaculture and ecological destruction. Although numerous iridovirus envelope proteins have been identified using bioinformatics and proteomic methods, their roles in virus infection remained largely unknown. Methods: Using SMART and TMHMM programs, we investigated the structural characteristics of Singapore grouper iridovirus (SGIV) VP19. A specific antibody against VP19 was generated and the expression profile of VP19 was clarified. The subcellular localization of VP19 in the absence or presence of other viral products was determined via transfection and immune fluorescence assay. In addition, Western blot assay and electron microscopy examination were performed to demonstrate whether SGIV VP19 was an envelope protein or a capsid protein. Results: Here, SGIV VP19 was cloned and characterized. Among all sequenced iridoviruses, VP19 and its orthologues shared common features, including 19 invariant cysteines, a proline-rich motif and a predicted transmembrane domain. Subsequently, the protein synthesis of VP19 was only detected at the late stage of SGIV infection and inhibited obviously by treating with AraC, confirming that VP19 was a late expressed protein. Ectopic expression of EGFP-VP19 in vitro displayed a punctate pattern in the cytoplasm. In SGIV infected cells, the newly synthesized VP19 protein was initially localized in the cytoplasm in a punctate pattern, and then aggregated into the virus assembly site at the late stage of SGIV infection, suggesting that other viral protein products were essential for VP19's function during SGIV infection. In addition, Western blot assay and electron microscopy observation revealed that SGIV VP19 was associated with viral envelope, which was different from major capsid protein (MCP). Conclusion: Taken together, the current data suggested that VP19 represented a conserved envelope protein in iridovirus, and might contribute greatly to virus assembly during virus infection.
英文摘要: description.abstract
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000330051600001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10945
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Characterization of an envelope gene VP19 from Singapore grouper iridovirus.pdf(1218KB)----开放获取View Download

Recommended Citation:
[Huang, Xiaohong; Huang, Youhua; Ouyang, Zhengliang; Wang, Shaowen; Chen, Xiuli; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Gong, Jie] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China.Characterization of an envelope gene VP19 from Singapore grouper iridovirus,VIROLOGY JOURNAL,2013,10():-354
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[[Huang, Xiaohong]'s Articles
[Huang, Youhua]'s Articles
[Ouyang, Zhengliang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[[Huang, Xiaohong]‘s Articles
[Huang, Youhua]‘s Articles
[Ouyang, Zhengliang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Characterization of an envelope gene VP19 from Singapore grouper iridovirus.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace