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Cloning, characterization and expression analysis of a caspase-8 like gene from the Hong Kong oyster, Crassostrea hongkongensis
[Xiang, Zhiming; Qu, Fufa; Zhang, Yang; Tong, Ying; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Qu, Fufa] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Qi, Lin] Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; carlzyu@scsio.ac.cn
2013
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume35Issue:6Pages:1797-1803
AbstractApoptosis plays a key role in many biological processes, including homeostasis within the immune system. A family of cysteine proteases, the caspases, constitutes the core of the apoptotic machinery. We have characterized the first bivalve caspase-8 ortholog from the Hong Kong oyster Crassostrea hongkongensis (designated ChCaspase-8). The full-length cDNA is 1945 bp in length encoding a putative protein of 557 amino acids that contains two N-terminal DED domains, and a CASc domain at the C-terminus. ChCaspase-8 is ubiquitously expressed in oysters, with highest expression levels in the gonad and labial palps. Following microbial infection, the expression of ChCaspase-8 increased in hemocytes from 12 to 72 h post-challenge. When expressed in HeLa cells, ChCaspase-8 is located in the cytoplasm, while over-expression of ChCaspase-8 in HEK293T cells activates the transcriptional activities of NF-kappa B. These results indicate that ChCaspase-8 might play an important role in the immune and apoptotic responses of oysters. (C) 2013 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordCrassostrea Hongkongensis Apoptosis Chcaspase-8 Nf-kappa b
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationThis research was supported by the National Science Foundation of China (No. 31202021 and 41176150), a National Basic Research Program of China (No. 2010CB126404) and a Guangdong-NSFC Joint Fund (U1201215). We thank Prof. Elizabeth De Stasio for her editing to the manuscript. ; This research was supported by the National Science Foundation of China (No. 31202021 and 41176150), a National Basic Research Program of China (No. 2010CB126404) and a Guangdong-NSFC Joint Fund (U1201215). We thank Prof. Elizabeth De Stasio for her editing to the manuscript. ; This research was supported by the National Science Foundation of China (No. 31202021 and 41176150), a National Basic Research Program of China (No. 2010CB126404) and a Guangdong-NSFC Joint Fund (U1201215). We thank Prof. Elizabeth De Stasio for her editing to the manuscript. ; This research was supported by the National Science Foundation of China (No. 31202021 and 41176150), a National Basic Research Program of China (No. 2010CB126404) and a Guangdong-NSFC Joint Fund (U1201215). We thank Prof. Elizabeth De Stasio for her editing to the manuscript.
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Language英语
Funding OrganizationThis research was supported by the National Science Foundation of China (No. 31202021 and 41176150), a National Basic Research Program of China (No. 2010CB126404) and a Guangdong-NSFC Joint Fund (U1201215). We thank Prof. Elizabeth De Stasio for her editing to the manuscript. ; This research was supported by the National Science Foundation of China (No. 31202021 and 41176150), a National Basic Research Program of China (No. 2010CB126404) and a Guangdong-NSFC Joint Fund (U1201215). We thank Prof. Elizabeth De Stasio for her editing to the manuscript. ; This research was supported by the National Science Foundation of China (No. 31202021 and 41176150), a National Basic Research Program of China (No. 2010CB126404) and a Guangdong-NSFC Joint Fund (U1201215). We thank Prof. Elizabeth De Stasio for her editing to the manuscript. ; This research was supported by the National Science Foundation of China (No. 31202021 and 41176150), a National Basic Research Program of China (No. 2010CB126404) and a Guangdong-NSFC Joint Fund (U1201215). We thank Prof. Elizabeth De Stasio for her editing to the manuscript.
WOS IDWOS:000330158800012
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10950
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorcarlzyu@scsio.ac.cn
Recommended Citation
GB/T 7714
[Xiang, Zhiming,Qu, Fufa,Zhang, Yang,et al. Cloning, characterization and expression analysis of a caspase-8 like gene from the Hong Kong oyster, Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(6):1797-1803.
APA [Xiang, Zhiming.,Qu, Fufa.,Zhang, Yang.,Tong, Ying.,Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.,...&carlzyu@scsio.ac.cn.(2013).Cloning, characterization and expression analysis of a caspase-8 like gene from the Hong Kong oyster, Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,35(6),1797-1803.
MLA [Xiang, Zhiming,et al."Cloning, characterization and expression analysis of a caspase-8 like gene from the Hong Kong oyster, Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 35.6(2013):1797-1803.
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