Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication
[Fu, Jian Ping; Luo, Zhang; Zou, Peng Fei; Zhang, Shu Huan; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Fu, Jian Ping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yan, Yang; Qin, Qi Wei] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China; pinnie@ihb.ac.cn
2013
发表期刊FISH PATHOLOGY
ISSN0388-788X
卷号48期号:4页码:126-134
摘要continuous cell line derived from extracranial carotid artery of the Chinese soft-shelled turtle Pelodiscus sinensis was established and characterized. It was named as STA (soft-shelled turtle artery) cell line. The cells were muscle-cell like, and were stained with antibody against smooth muscle myofilament marker, a-smooth muscle actin. These cells had a normal diploid chromosome number of 2n = 66, and their 12S rRNA and 16S rRNA sequences shared 99% identity with ones of the turtle in database. The cell line can be cultured well in media DMEM/F12 or M199 supplemented with 10% FBS at 28 C. It was further demonstrated that the cells were transfected successfully with pTurbo plasmid, with a transfection efficiency being over 30%. The soft-shelled turtle iridovirus (STIV) propagated in the cell line, causing typical CPE with the formation of inclusion bodies. It is suggested that the established STA cell line provides a convenient platform for studying the pathogenesis of STIV and biological aspects of the turtle.
部门归属LMB
关键词Cell Line Pelodiscus Sinensis Chinese Soft-shelled Turtle Transfection Soft-shelled Turtle Iridovirus Stiv
学科领域Fisheries ; Veterinary Sciences
资助者The research was financially supported by a grant (31230075) from the National Natural Science Foundation of China and a grant (2009CB118703) from National Basic Research of Program (973 Program). ; The research was financially supported by a grant (31230075) from the National Natural Science Foundation of China and a grant (2009CB118703) from National Basic Research of Program (973 Program). ; The research was financially supported by a grant (31230075) from the National Natural Science Foundation of China and a grant (2009CB118703) from National Basic Research of Program (973 Program). ; The research was financially supported by a grant (31230075) from the National Natural Science Foundation of China and a grant (2009CB118703) from National Basic Research of Program (973 Program).
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资助者The research was financially supported by a grant (31230075) from the National Natural Science Foundation of China and a grant (2009CB118703) from National Basic Research of Program (973 Program). ; The research was financially supported by a grant (31230075) from the National Natural Science Foundation of China and a grant (2009CB118703) from National Basic Research of Program (973 Program). ; The research was financially supported by a grant (31230075) from the National Natural Science Foundation of China and a grant (2009CB118703) from National Basic Research of Program (973 Program). ; The research was financially supported by a grant (31230075) from the National Natural Science Foundation of China and a grant (2009CB118703) from National Basic Research of Program (973 Program).
WOS记录号WOS:000329960200003
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10953
专题中科院海洋生物资源可持续利用重点实验室
通讯作者pinnie@ihb.ac.cn
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GB/T 7714
[Fu, Jian Ping,Luo, Zhang,Zou, Peng Fei,et al. Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication[J]. FISH PATHOLOGY,2013,48(4):126-134.
APA [Fu, Jian Ping.,Luo, Zhang.,Zou, Peng Fei.,Zhang, Shu Huan.,Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.,...&pinnie@ihb.ac.cn.(2013).Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication.FISH PATHOLOGY,48(4),126-134.
MLA [Fu, Jian Ping,et al."Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication".FISH PATHOLOGY 48.4(2013):126-134.
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