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学科主题: Fisheries ; Veterinary Sciences
题名: Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication
作者: [Fu, Jian Ping ; Luo, Zhang ; Zou, Peng Fei ; Zhang, Shu Huan ; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China ; [Fu, Jian Ping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Yan, Yang ; Qin, Qi Wei] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China
通讯作者: pinnie@ihb.ac.cn
关键词: cell line ; Pelodiscus sinensis ; Chinese soft-shelled turtle ; transfection ; soft-shelled turtle iridovirus ; STIV
刊名: FISH PATHOLOGY
发表日期: 2013
卷: 48, 期:4, 页:126-134
部门归属: LMB
资助者: The research was financially supported by a grant (31230075) from the National Natural Science Foundation of China and a grant (2009CB118703) from National Basic Research of Program (973 Program).
摘要: continuous cell line derived from extracranial carotid artery of the Chinese soft-shelled turtle Pelodiscus sinensis was established and characterized. It was named as STA (soft-shelled turtle artery) cell line. The cells were muscle-cell like, and were stained with antibody against smooth muscle myofilament marker, a-smooth muscle actin. These cells had a normal diploid chromosome number of 2n = 66, and their 12S rRNA and 16S rRNA sequences shared 99% identity with ones of the turtle in database. The cell line can be cultured well in media DMEM/F12 or M199 supplemented with 10% FBS at 28 C. It was further demonstrated that the cells were transfected successfully with pTurbo plasmid, with a transfection efficiency being over 30%. The soft-shelled turtle iridovirus (STIV) propagated in the cell line, causing typical CPE with the formation of inclusion bodies. It is suggested that the established STA cell line provides a convenient platform for studying the pathogenesis of STIV and biological aspects of the turtle.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000329960200003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10953
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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[Fu, Jian Ping; Luo, Zhang; Zou, Peng Fei; Zhang, Shu Huan; Nie, Pin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Fu, Jian Ping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yan, Yang; Qin, Qi Wei] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Nie, Pin] Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China.Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication,FISH PATHOLOGY,2013,48(4):126-134
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文件名: Establishment of a Cell Line from Chinese Soft-shelled Turtle Pelodiscus sinensis with the Practicability of Transfection and Viral Replication.pdf
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