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An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication
[Yan, Yang; Guo, Chuanyu; Huang, Xiaohong; Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Cui, Huachun] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA; [Guo, Chuanyu] Univ Chinese Acad Sci, Beijing, Peoples R China; [Li, Jun] Sun Yat Sen Univ, Zhongshan Sch Med, Inst Human Virol, Guangzhou 510080, Guangdong, Peoples R China; qinqw@scsio.ac.cn
2013
Source PublicationJOURNAL OF GENERAL VIROLOGY
ISSN0022-1317
Volume94Issue:12Pages:2759-2770
AbstractInsulin-like growth factors (IGFs) play crucial roles in regulating cell differentiation, proliferation and apoptosis. In this study, a novel IGF homologue gene (IGF-like) encoded by Singapore grouper iridovirus (SGIV) ORF062R (termed SGIV-IGF), was cloned and characterized. The coding region of SGIV-IGF is 771 bp in length, with a variable number of tandem repeats (VNTR) locus at the 3'-end. We cloned one isoform of this novel gene, 582 bp in length, containing the predicted IGF domain and 3.6 copy numbers of the 27 bp repeat unit. SGIV-IGF was an early transcribed gene during viral infection, and SGIV-IGF was distributed predominantly in the cytoplasm with a diffused granular appearance. Intriguingly, overexpression of SGIV-IGF was able to promote the growth of grouper embryonic cells (GP cells) by promoting G1/S phase transition, which was at least partially dependent on its 3'-end VNTR locus. Furthermore, viral titre assay and real-time quantitative PCR (RT-qPCR) analysis proved that SGIV-IGF could promote SGIV replication in grouper cells. In addition, overexpression of SGIV-IGF mildly facilitated apoptosis in SGIV-infected non-host fathead minnow (FHM) cells. Together, our study demonstrated a novel functional gene of SGIV which may regulate viral replication and cellular processes through multiple mechanisms that appear to be cell type-dependent.
DepartmentLMB
Subject AreaBiotechnology & Applied Microbiology ; Virology
Funding OrganizationThis work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213).
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Language英语
Funding OrganizationThis work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213).
WOS IDWOS:000328808000021
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10955
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorqinqw@scsio.ac.cn
Recommended Citation
GB/T 7714
[Yan, Yang,Guo, Chuanyu,Huang, Xiaohong,et al. An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication[J]. JOURNAL OF GENERAL VIROLOGY,2013,94(12):2759-2770.
APA [Yan, Yang.,Guo, Chuanyu.,Huang, Xiaohong.,Wei, Jingguang.,Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.,...&qinqw@scsio.ac.cn.(2013).An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication.JOURNAL OF GENERAL VIROLOGY,94(12),2759-2770.
MLA [Yan, Yang,et al."An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication".JOURNAL OF GENERAL VIROLOGY 94.12(2013):2759-2770.
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