An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication
[Yan, Yang; Guo, Chuanyu; Huang, Xiaohong; Wei, Jingguang; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Cui, Huachun] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA; [Guo, Chuanyu] Univ Chinese Acad Sci, Beijing, Peoples R China; [Li, Jun] Sun Yat Sen Univ, Zhongshan Sch Med, Inst Human Virol, Guangzhou 510080, Guangdong, Peoples R China; qinqw@scsio.ac.cn
2013
发表期刊JOURNAL OF GENERAL VIROLOGY
ISSN0022-1317
卷号94期号:12页码:2759-2770
摘要Insulin-like growth factors (IGFs) play crucial roles in regulating cell differentiation, proliferation and apoptosis. In this study, a novel IGF homologue gene (IGF-like) encoded by Singapore grouper iridovirus (SGIV) ORF062R (termed SGIV-IGF), was cloned and characterized. The coding region of SGIV-IGF is 771 bp in length, with a variable number of tandem repeats (VNTR) locus at the 3'-end. We cloned one isoform of this novel gene, 582 bp in length, containing the predicted IGF domain and 3.6 copy numbers of the 27 bp repeat unit. SGIV-IGF was an early transcribed gene during viral infection, and SGIV-IGF was distributed predominantly in the cytoplasm with a diffused granular appearance. Intriguingly, overexpression of SGIV-IGF was able to promote the growth of grouper embryonic cells (GP cells) by promoting G1/S phase transition, which was at least partially dependent on its 3'-end VNTR locus. Furthermore, viral titre assay and real-time quantitative PCR (RT-qPCR) analysis proved that SGIV-IGF could promote SGIV replication in grouper cells. In addition, overexpression of SGIV-IGF mildly facilitated apoptosis in SGIV-infected non-host fathead minnow (FHM) cells. Together, our study demonstrated a novel functional gene of SGIV which may regulate viral replication and cellular processes through multiple mechanisms that appear to be cell type-dependent.
部门归属LMB
学科领域Biotechnology & Applied Microbiology ; Virology
资助者This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213).
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资助者This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213). ; This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402), Natural Science Foundation of China (41206137, 31330082), and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ201116, KZCX2-EW-Q213).
WOS记录号WOS:000328808000021
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10955
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
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[Yan, Yang,Guo, Chuanyu,Huang, Xiaohong,et al. An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication[J]. JOURNAL OF GENERAL VIROLOGY,2013,94(12):2759-2770.
APA [Yan, Yang.,Guo, Chuanyu.,Huang, Xiaohong.,Wei, Jingguang.,Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China.,...&qinqw@scsio.ac.cn.(2013).An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication.JOURNAL OF GENERAL VIROLOGY,94(12),2759-2770.
MLA [Yan, Yang,et al."An insulin-like growth factor homologue of Singapore grouper iridovirus modulates cell proliferation, apoptosis and enhances viral replication".JOURNAL OF GENERAL VIROLOGY 94.12(2013):2759-2770.
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